Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway
Summary: The surface structures of archaeal cells, many of which exist at high temperatures, high salinity, and non-physiological pH, are key factors for their adaptation to extreme living conditions. In the haloarchaeon Haloarcula hispanica, we have discovered a thin filamentous surface appendage c...
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Elsevier
2025-02-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225000525 |
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| author | Anna V. Galeva Dahe Zhao Alexey S. Syutkin Marina Yu Topilina Sergei Yu Shchyogolev Elena Yu Pavlova Olga M. Selivanova Igor I. Kireev Alexey K. Surin Gennady L. Burygin Jingfang Liu Hua Xiang Mikhail G. Pyatibratov |
| author_facet | Anna V. Galeva Dahe Zhao Alexey S. Syutkin Marina Yu Topilina Sergei Yu Shchyogolev Elena Yu Pavlova Olga M. Selivanova Igor I. Kireev Alexey K. Surin Gennady L. Burygin Jingfang Liu Hua Xiang Mikhail G. Pyatibratov |
| author_sort | Anna V. Galeva |
| collection | DOAJ |
| description | Summary: The surface structures of archaeal cells, many of which exist at high temperatures, high salinity, and non-physiological pH, are key factors for their adaptation to extreme living conditions. In the haloarchaeon Haloarcula hispanica, we have discovered a thin filamentous surface appendage called tat-fimbriae (''tafi''), which were identified to be composed of three protein subunits, TafA, TafC, and TafE, among which TafA is the major fimbrial subunit. Molecular genetic evidence demonstrates TafA was transported through the twin-arginine translocation pathway (Tat-pathway). Based on protein structure prediction (including AlphaFold 3), tafi exhibits a linear structure: TafC at the tip, TafE acting as an adapter, TafA forming the core filament, and they link the fourth subunit TafF, anchoring tafi to the cell wall. To our knowledge, this is the first case that the Tat-pathway has been linked to the secretion of protein subunits forming prokaryotic filamentous structures. |
| format | Article |
| id | doaj-art-e92eb63e77ee42bca78ec31150d2a0d5 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-e92eb63e77ee42bca78ec31150d2a0d52025-01-31T05:12:11ZengElsevieriScience2589-00422025-02-01282111793Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathwayAnna V. Galeva0Dahe Zhao1Alexey S. Syutkin2Marina Yu Topilina3Sergei Yu Shchyogolev4Elena Yu Pavlova5Olga M. Selivanova6Igor I. Kireev7Alexey K. Surin8Gennady L. Burygin9Jingfang Liu10Hua Xiang11Mikhail G. Pyatibratov12Institute of Protein Research, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, Moscow Region 142290, RussiaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaInstitute of Protein Research, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, Moscow Region 142290, RussiaInstitute of Protein Research, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, Moscow Region 142290, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Prospekt Entuziastov 13, Saratov 410049, RussiaInstitute of Protein Research, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, Moscow Region 142290, RussiaInstitute of Protein Research, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, Moscow Region 142290, RussiaA.N. Belozersky Institute of Physico-chemical Biology, M.V. Lomonosov Moscow State University, Leninskie Gori 1, Bldg 40, Moscow 119234, RussiaInstitute of Protein Research, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, Moscow Region 142290, Russia; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki 6, Pushchino, Moscow Region 142290, Russia; State Research Center for Applied Microbiology & Biotechnology, Obolensk, Serpukhov District, Moscow Region 142279, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Prospekt Entuziastov 13, Saratov 410049, Russia; Vavilov Saratov State Agrarian University, 1 Teatralnaya Ploshchad, Saratov 410012, RussiaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Corresponding authorInstitute of Protein Research, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, Moscow Region 142290, Russia; Corresponding authorSummary: The surface structures of archaeal cells, many of which exist at high temperatures, high salinity, and non-physiological pH, are key factors for their adaptation to extreme living conditions. In the haloarchaeon Haloarcula hispanica, we have discovered a thin filamentous surface appendage called tat-fimbriae (''tafi''), which were identified to be composed of three protein subunits, TafA, TafC, and TafE, among which TafA is the major fimbrial subunit. Molecular genetic evidence demonstrates TafA was transported through the twin-arginine translocation pathway (Tat-pathway). Based on protein structure prediction (including AlphaFold 3), tafi exhibits a linear structure: TafC at the tip, TafE acting as an adapter, TafA forming the core filament, and they link the fourth subunit TafF, anchoring tafi to the cell wall. To our knowledge, this is the first case that the Tat-pathway has been linked to the secretion of protein subunits forming prokaryotic filamentous structures.http://www.sciencedirect.com/science/article/pii/S2589004225000525Molecular biologyMicrobiologyCell biology |
| spellingShingle | Anna V. Galeva Dahe Zhao Alexey S. Syutkin Marina Yu Topilina Sergei Yu Shchyogolev Elena Yu Pavlova Olga M. Selivanova Igor I. Kireev Alexey K. Surin Gennady L. Burygin Jingfang Liu Hua Xiang Mikhail G. Pyatibratov Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway iScience Molecular biology Microbiology Cell biology |
| title | Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway |
| title_full | Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway |
| title_fullStr | Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway |
| title_full_unstemmed | Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway |
| title_short | Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway |
| title_sort | tat fimbriae tafi an unusual type of haloarchaeal surface structure depending on the twin arginine translocation pathway |
| topic | Molecular biology Microbiology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225000525 |
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