Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China
Abstract Background Diabetic foot ulcers (DFUs) are characterized by dynamic wound microbiome, the timely and accurate identification of pathogens in the clinic is required to initiate precise and individualized treatment. Metagenomic next-generation sequencing (mNGS) has been a useful supplement to...
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BMC
2025-01-01
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| Series: | BMC Endocrine Disorders |
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| Online Access: | https://doi.org/10.1186/s12902-025-01837-z |
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| author | Hongteng Xie Zhaohong Chen Guohua Wu Pei Wei Teng Gong Shun Chen Zhaorong Xu |
| author_facet | Hongteng Xie Zhaohong Chen Guohua Wu Pei Wei Teng Gong Shun Chen Zhaorong Xu |
| author_sort | Hongteng Xie |
| collection | DOAJ |
| description | Abstract Background Diabetic foot ulcers (DFUs) are characterized by dynamic wound microbiome, the timely and accurate identification of pathogens in the clinic is required to initiate precise and individualized treatment. Metagenomic next-generation sequencing (mNGS) has been a useful supplement to routine culture method for the etiological diagnosis of DFUs. In this study, we utilized a routine culture method and mNGS to analyze the same DFU wound samples and the results were compared. Methods Forty samples from patients with DFUs at a tertiary medical center in South China were collected, the microorganisms were identified with mNGS and routine culture method simultaneously. Results The results showed that the positive detection rate of microorganisms in DFUs with mNGS was much higher (95% vs. 60%). Thirteen strains of microorganisms were detected with routine culture method, and seventy-seven strains were detected with mNGS. Staphylococcus aureus was the most common microorganism detected with culture method, while Enterococcus faecalis was the most common microorganism detected with mNGS. The false negative rate of the culture method was 35%, that was, 14 samples with negative results with culture method were found to be positive with mNGS. Conclusion The mNGS method had a higher positive detection rate and identified a broader spectrum of microorganisms in DFUs, thus, mNGS provided a more comprehensive understanding of the microbiome of DFUs to facilitate the development of timely and optimal treatment. Trial registration The study was conducted in accordance with the Declaration of Helsinki and approved by the Ethical Review Committee of the Fujian Medical University Union Hospital (approval number 2021KY054). |
| format | Article |
| id | doaj-art-e90429e6a6d441e69ba79d670c3a6abc |
| institution | Kabale University |
| issn | 1472-6823 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Endocrine Disorders |
| spelling | doaj-art-e90429e6a6d441e69ba79d670c3a6abc2025-01-26T12:37:40ZengBMCBMC Endocrine Disorders1472-68232025-01-0125111010.1186/s12902-025-01837-zApplication of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South ChinaHongteng Xie0Zhaohong Chen1Guohua Wu2Pei Wei3Teng Gong4Shun Chen5Zhaorong Xu6Burn and Wound Repair Department, Fujian Medical University Union HospitalBurn and Wound Repair Department, Fujian Medical University Union HospitalBurn and Wound Repair Department, Fujian Medical University Union HospitalBurn and Wound Repair Department, Fujian Medical University Union HospitalBurn and Wound Repair Department, Fujian Medical University Union HospitalBurn and Wound Repair Department, Fujian Medical University Union HospitalBurn and Wound Repair Department, Fujian Medical University Union HospitalAbstract Background Diabetic foot ulcers (DFUs) are characterized by dynamic wound microbiome, the timely and accurate identification of pathogens in the clinic is required to initiate precise and individualized treatment. Metagenomic next-generation sequencing (mNGS) has been a useful supplement to routine culture method for the etiological diagnosis of DFUs. In this study, we utilized a routine culture method and mNGS to analyze the same DFU wound samples and the results were compared. Methods Forty samples from patients with DFUs at a tertiary medical center in South China were collected, the microorganisms were identified with mNGS and routine culture method simultaneously. Results The results showed that the positive detection rate of microorganisms in DFUs with mNGS was much higher (95% vs. 60%). Thirteen strains of microorganisms were detected with routine culture method, and seventy-seven strains were detected with mNGS. Staphylococcus aureus was the most common microorganism detected with culture method, while Enterococcus faecalis was the most common microorganism detected with mNGS. The false negative rate of the culture method was 35%, that was, 14 samples with negative results with culture method were found to be positive with mNGS. Conclusion The mNGS method had a higher positive detection rate and identified a broader spectrum of microorganisms in DFUs, thus, mNGS provided a more comprehensive understanding of the microbiome of DFUs to facilitate the development of timely and optimal treatment. Trial registration The study was conducted in accordance with the Declaration of Helsinki and approved by the Ethical Review Committee of the Fujian Medical University Union Hospital (approval number 2021KY054).https://doi.org/10.1186/s12902-025-01837-zDiabetic foot ulcerMetagenomic next-generation sequencingMicroorganism cultureWound microbiome |
| spellingShingle | Hongteng Xie Zhaohong Chen Guohua Wu Pei Wei Teng Gong Shun Chen Zhaorong Xu Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China BMC Endocrine Disorders Diabetic foot ulcer Metagenomic next-generation sequencing Microorganism culture Wound microbiome |
| title | Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China |
| title_full | Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China |
| title_fullStr | Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China |
| title_full_unstemmed | Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China |
| title_short | Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China |
| title_sort | application of metagenomic next generation sequencing mngs to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in south china |
| topic | Diabetic foot ulcer Metagenomic next-generation sequencing Microorganism culture Wound microbiome |
| url | https://doi.org/10.1186/s12902-025-01837-z |
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