Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia
Despite continuous attempts to improve therapy, the outcomes of acute myeloid leukemia remain almost unchanged over last decades. Drugs made with a more complete understanding of the biology of acute myeloid leukemia do not equal the hopes for better prognosis. The best results are achieved only wit...
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| Format: | Article |
| Language: | Russian |
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2019-07-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/349 |
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| author | A. S. Polyakov Ya. A. Noskov Yu. V. Nikitin V. V. Tyrenko S. N. Kolubaeva A. N. Bogdanov V. N. Semelev O. R. Petrova S. V. Bondarchuk D. K. Zhogolev A. D. Zolotarev A. V. Kovalev Yu. E. Puchkova |
| author_facet | A. S. Polyakov Ya. A. Noskov Yu. V. Nikitin V. V. Tyrenko S. N. Kolubaeva A. N. Bogdanov V. N. Semelev O. R. Petrova S. V. Bondarchuk D. K. Zhogolev A. D. Zolotarev A. V. Kovalev Yu. E. Puchkova |
| author_sort | A. S. Polyakov |
| collection | DOAJ |
| description | Despite continuous attempts to improve therapy, the outcomes of acute myeloid leukemia remain almost unchanged over last decades. Drugs made with a more complete understanding of the biology of acute myeloid leukemia do not equal the hopes for better prognosis. The best results are achieved only with high-dose chemotherapy, which is only possible for a limited number of patients. High phenotypic and genotypic heterogeneity of acute myeloid leukemia defines the relevance to develop personalized approaches to therapy, including those based on determination of individual drug sensitivity of blast cells.This article presents the results of developing an ex-vivo model of acute myeloid leukemia, as well as testing of two in vitro sensitivity assessment methods: evaluation of the genotoxicity of drugs in the micronucleus test and vitality and sensitivity to chemotherapy in sorted blast cells. Prospects of individualized therapy of acute myeloid leukemia were determined based on introduction into clinical practice and continuing the research. |
| format | Article |
| id | doaj-art-e8d76bc63efc4d4898fad401b59758c0 |
| institution | Kabale University |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2019-07-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-e8d76bc63efc4d4898fad401b59758c02025-08-20T03:35:33ZrusABV-pressОнкогематология1818-83462413-40232019-07-01142596910.17650/1818-8346-2019-14-2-59-69314Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemiaA. S. Polyakov0Ya. A. Noskov1Yu. V. Nikitin2V. V. Tyrenko3S. N. Kolubaeva4A. N. Bogdanov5V. N. Semelev6O. R. Petrova7S. V. Bondarchuk8D. K. Zhogolev9A. D. Zolotarev10A. V. Kovalev11Yu. E. Puchkova12Military Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaMilitary Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaDespite continuous attempts to improve therapy, the outcomes of acute myeloid leukemia remain almost unchanged over last decades. Drugs made with a more complete understanding of the biology of acute myeloid leukemia do not equal the hopes for better prognosis. The best results are achieved only with high-dose chemotherapy, which is only possible for a limited number of patients. High phenotypic and genotypic heterogeneity of acute myeloid leukemia defines the relevance to develop personalized approaches to therapy, including those based on determination of individual drug sensitivity of blast cells.This article presents the results of developing an ex-vivo model of acute myeloid leukemia, as well as testing of two in vitro sensitivity assessment methods: evaluation of the genotoxicity of drugs in the micronucleus test and vitality and sensitivity to chemotherapy in sorted blast cells. Prospects of individualized therapy of acute myeloid leukemia were determined based on introduction into clinical practice and continuing the research.https://oncohematology.abvpress.ru/ongm/article/view/349acute myeloid leukemiasecondary acute myeloid leukemiaex vivo modeldrug sensitivity testinggenotoxicitymicronucleus testfluorescence activated cell sortingindividualization of drug therapy |
| spellingShingle | A. S. Polyakov Ya. A. Noskov Yu. V. Nikitin V. V. Tyrenko S. N. Kolubaeva A. N. Bogdanov V. N. Semelev O. R. Petrova S. V. Bondarchuk D. K. Zhogolev A. D. Zolotarev A. V. Kovalev Yu. E. Puchkova Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia Онкогематология acute myeloid leukemia secondary acute myeloid leukemia ex vivo model drug sensitivity testing genotoxicity micronucleus test fluorescence activated cell sorting individualization of drug therapy |
| title | Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia |
| title_full | Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia |
| title_fullStr | Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia |
| title_full_unstemmed | Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia |
| title_short | Ex vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia |
| title_sort | ex vivo model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemia |
| topic | acute myeloid leukemia secondary acute myeloid leukemia ex vivo model drug sensitivity testing genotoxicity micronucleus test fluorescence activated cell sorting individualization of drug therapy |
| url | https://oncohematology.abvpress.ru/ongm/article/view/349 |
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