Protocol for constructing in vivo and in vitro models integrating clinical and proteomic subtypes of chordoma

Summary: Here, we present a protocol for constructing both in vivo and in vitro models of chordoma, integrating clinical and proteomic subtyping to enable a comprehensive analysis. We detail the steps for tissue sample preparation and tandem mass tag proteomic analysis and apply non-negative matrix...

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Bibliographic Details
Main Authors: Jianxuan Gao, Jinbo Hu, Tong Meng
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S266616672500125X
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