Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle
The aim of this study was to investigate the period of estrus cycle in aceh cattle, Indonesia, based on vaginal cytology techniques. Four healthy females of aceh cattle with average weight of 250–300 kg, age of 5–7 years, and body condition score of 3-4 were used. All cattle were subjected to ultras...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2016/3976125 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564510849761280 |
|---|---|
| author | Tongku N. Siregar Juli Melia Rohaya Cut Nila Thasmi Dian Masyitha Sri Wahyuni Juliana Rosa Nurhafni Budianto Panjaitan Herrialfian |
| author_facet | Tongku N. Siregar Juli Melia Rohaya Cut Nila Thasmi Dian Masyitha Sri Wahyuni Juliana Rosa Nurhafni Budianto Panjaitan Herrialfian |
| author_sort | Tongku N. Siregar |
| collection | DOAJ |
| description | The aim of this study was to investigate the period of estrus cycle in aceh cattle, Indonesia, based on vaginal cytology techniques. Four healthy females of aceh cattle with average weight of 250–300 kg, age of 5–7 years, and body condition score of 3-4 were used. All cattle were subjected to ultrasonography analysis for the occurrence of corpus luteum before being synchronized using intramuscular injections of PGF2 alpha 25 mg. A vaginal swab was collected from aceh cattle, stained with Giemsa 10%, and observed microscopically. Period of estrus cycle was predicted from day 1 to day 24 after estrus synchronization was confirmed using ultrasonography analysis at the same day. The result showed that parabasal, intermediary, and superficial epithelium were found in the vaginal swabs collected from proestrus, metestrus, and diestrus aceh cattle. Proportions of these cells in the particular period of estrus cycle were 36.22, 32.62, and 31.16 (proestrus); 21.33, 32.58, and 46.09 (estrus); 40.75, 37.58, and 21.67 (metestrus); and 41.07, 37.38, and 21.67 (diestrus), respectively. In conclusion, dominant proportion of superficial cell that occurred in estrus period might be used as the base for determining optimal time for insemination. |
| format | Article |
| id | doaj-art-e872238b9fcb4652b64d218d7b551ad2 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-e872238b9fcb4652b64d218d7b551ad22025-02-03T01:10:58ZengWileyVeterinary Medicine International2090-81132042-00482016-01-01201610.1155/2016/39761253976125Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh CattleTongku N. Siregar0Juli Melia1Rohaya2Cut Nila Thasmi3Dian Masyitha4Sri Wahyuni5Juliana Rosa6Nurhafni7Budianto Panjaitan8Herrialfian9Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaThe aim of this study was to investigate the period of estrus cycle in aceh cattle, Indonesia, based on vaginal cytology techniques. Four healthy females of aceh cattle with average weight of 250–300 kg, age of 5–7 years, and body condition score of 3-4 were used. All cattle were subjected to ultrasonography analysis for the occurrence of corpus luteum before being synchronized using intramuscular injections of PGF2 alpha 25 mg. A vaginal swab was collected from aceh cattle, stained with Giemsa 10%, and observed microscopically. Period of estrus cycle was predicted from day 1 to day 24 after estrus synchronization was confirmed using ultrasonography analysis at the same day. The result showed that parabasal, intermediary, and superficial epithelium were found in the vaginal swabs collected from proestrus, metestrus, and diestrus aceh cattle. Proportions of these cells in the particular period of estrus cycle were 36.22, 32.62, and 31.16 (proestrus); 21.33, 32.58, and 46.09 (estrus); 40.75, 37.58, and 21.67 (metestrus); and 41.07, 37.38, and 21.67 (diestrus), respectively. In conclusion, dominant proportion of superficial cell that occurred in estrus period might be used as the base for determining optimal time for insemination.http://dx.doi.org/10.1155/2016/3976125 |
| spellingShingle | Tongku N. Siregar Juli Melia Rohaya Cut Nila Thasmi Dian Masyitha Sri Wahyuni Juliana Rosa Nurhafni Budianto Panjaitan Herrialfian Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle Veterinary Medicine International |
| title | Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle |
| title_full | Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle |
| title_fullStr | Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle |
| title_full_unstemmed | Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle |
| title_short | Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle |
| title_sort | determining proportion of exfoliative vaginal cell during various stages of estrus cycle using vaginal cytology techniques in aceh cattle |
| url | http://dx.doi.org/10.1155/2016/3976125 |
| work_keys_str_mv | AT tongkunsiregar determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT julimelia determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT rohaya determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT cutnilathasmi determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT dianmasyitha determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT sriwahyuni determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT julianarosa determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT nurhafni determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT budiantopanjaitan determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle AT herrialfian determiningproportionofexfoliativevaginalcellduringvariousstagesofestruscycleusingvaginalcytologytechniquesinacehcattle |