Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamics
BackgroundThe convergence of macrophage-targeted strategies with immune checkpoint blockade therapies defines a pivotal avenue in contemporary tumor therapy. Identifying robust genetic regulators in this context is imperative.MethodsThis study elucidates IFI30's role in enhancing Major Histocom...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1523854/full |
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| author | Lihe Jiang Lihe Jiang Lihe Jiang Peili Wang Peili Wang Yixuan Hou Jingying Chen Hua Li Hua Li |
| author_facet | Lihe Jiang Lihe Jiang Lihe Jiang Peili Wang Peili Wang Yixuan Hou Jingying Chen Hua Li Hua Li |
| author_sort | Lihe Jiang |
| collection | DOAJ |
| description | BackgroundThe convergence of macrophage-targeted strategies with immune checkpoint blockade therapies defines a pivotal avenue in contemporary tumor therapy. Identifying robust genetic regulators in this context is imperative.MethodsThis study elucidates IFI30's role in enhancing Major Histocompatibility Complex II (MHC-II) restriction antigen processing. Despite its recognition in cancer immunotherapy, IFI30 remains a nascent focus. Our approach involves a multi-omics analysis of IFI30 tumor immunological profile in the macrophage-mediated Tumor Microenvironment (TME), spanning various cancers and bolstered by rigorous co-culture laboratory work.ResultsIFI30 predominantly localizes in monocyte/macrophage populations, correlating strongly with immune cell infiltration. Substantiated by single-cell analysis, IFI30 exhibits significant functional enrichment in immune-related pathways. Co-expression with immune-related genes, including MHC elements and immune checkpoints, further validates its relevance.ConclusionOur study positions IFI30 as a promising immunotherapeutic target. Pan-cancer analyses and glioblastoma multiforme (GBM) investigations collectively underscore IFI30's potential as a TME modulator, particularly in its interaction with M2-macrophages. IFI30 emerges as a prospective intervention point in the immunotherapeutic landscape. |
| format | Article |
| id | doaj-art-e779006e5ef7495e9f861d6fefe19b9b |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-e779006e5ef7495e9f861d6fefe19b9b2025-01-24T07:13:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011610.3389/fimmu.2025.15238541523854Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamicsLihe Jiang0Lihe Jiang1Lihe Jiang2Peili Wang3Peili Wang4Yixuan Hou5Jingying Chen6Hua Li7Hua Li8School of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, Guangxi, ChinaMedical College, Guangxi University, Nanning, Guangxi, ChinaKey Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou, ChinaSchool of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, Guangxi, ChinaMedical College, Guangxi University, Nanning, Guangxi, ChinaSchool of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, Guangxi, ChinaDepartment of General Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, ChinaDepartment of General Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, ChinaKey Laboratory of Tumor Molecular Pathology of Baise, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, ChinaBackgroundThe convergence of macrophage-targeted strategies with immune checkpoint blockade therapies defines a pivotal avenue in contemporary tumor therapy. Identifying robust genetic regulators in this context is imperative.MethodsThis study elucidates IFI30's role in enhancing Major Histocompatibility Complex II (MHC-II) restriction antigen processing. Despite its recognition in cancer immunotherapy, IFI30 remains a nascent focus. Our approach involves a multi-omics analysis of IFI30 tumor immunological profile in the macrophage-mediated Tumor Microenvironment (TME), spanning various cancers and bolstered by rigorous co-culture laboratory work.ResultsIFI30 predominantly localizes in monocyte/macrophage populations, correlating strongly with immune cell infiltration. Substantiated by single-cell analysis, IFI30 exhibits significant functional enrichment in immune-related pathways. Co-expression with immune-related genes, including MHC elements and immune checkpoints, further validates its relevance.ConclusionOur study positions IFI30 as a promising immunotherapeutic target. Pan-cancer analyses and glioblastoma multiforme (GBM) investigations collectively underscore IFI30's potential as a TME modulator, particularly in its interaction with M2-macrophages. IFI30 emerges as a prospective intervention point in the immunotherapeutic landscape.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1523854/fullIFI30scRNA-seqspatial transcriptomicspan-cancermacrophageimmunotherapy |
| spellingShingle | Lihe Jiang Lihe Jiang Lihe Jiang Peili Wang Peili Wang Yixuan Hou Jingying Chen Hua Li Hua Li Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamics Frontiers in Immunology IFI30 scRNA-seq spatial transcriptomics pan-cancer macrophage immunotherapy |
| title | Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamics |
| title_full | Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamics |
| title_fullStr | Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamics |
| title_full_unstemmed | Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamics |
| title_short | Comprehensive single-cell pan-cancer atlas unveils IFI30+ macrophages as key modulators of intra-tumoral immune dynamics |
| title_sort | comprehensive single cell pan cancer atlas unveils ifi30 macrophages as key modulators of intra tumoral immune dynamics |
| topic | IFI30 scRNA-seq spatial transcriptomics pan-cancer macrophage immunotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1523854/full |
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