Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway
Introduction: The effects of remimazolam (Re) in combination with andrographolide (AP) on learning, memory, and motor abilities in rats following cardiopulmonary bypass (CPB) surgery were studied. Methods: We hy...
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IMR Press
2025-01-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://www.imrpress.com/journal/JIN/24/1/10.31083/JIN25665 |
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| author | Chong Chen Lixia Lan Kai Xu |
| author_facet | Chong Chen Lixia Lan Kai Xu |
| author_sort | Chong Chen |
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| description | Introduction: The effects of remimazolam (Re) in combination with andrographolide (AP) on learning, memory, and motor abilities in rats following cardiopulmonary bypass (CPB) surgery were studied. Methods: We hypothesized that the combination of Re and AP could improve postoperative cognitive dysfunction (POCD) in rats after CPB by modulating nervous system inflammation. Cognitive function was assessed using the Morris Water Maze test, and the concentrations of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) in serum were measured by enzyme-linked immunosorbent assay (ELISA). Apoptosis was evaluated using western blotting and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining assay. Results: The results indicated that both Re and AP independently improved cognitive function in rats after CPB and inhibited the secretion of inflammatory factors and apoptosis in hippocampal tissues. Combined administration of Re and AP enhanced the alleviation of POCD compared with monotherapy. The adenosine monophosphate-activated protein kinase/silent information regulator of transcription 1 (AMPK/SIRT1) signaling pathway was activated by the combination of Re and AP. Conclusions: Collectively, the combination of Re and AP treatment significantly improves POCD in rats after CPB through activation of the AMPK/SIRT1 signaling pathway. |
| format | Article |
| id | doaj-art-e67abc54e8c34281bf65c33ff2c97916 |
| institution | Kabale University |
| issn | 0219-6352 1757-448X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IMR Press |
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| series | Journal of Integrative Neuroscience |
| spelling | doaj-art-e67abc54e8c34281bf65c33ff2c979162025-01-25T07:38:07ZengIMR PressJournal of Integrative Neuroscience0219-63521757-448X2025-01-012412566510.31083/JIN25665S0219-6352(24)00851-9Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling PathwayChong Chen0Lixia Lan1Kai Xu2Department of Anesthesiology, The Second Affiliated Hospital, Zhejiang University, School of Medicine, 310009 Hangzhou, Zhejiang, ChinaDepartment of Anesthesia, Lishui Maternal and Child Health Center, 323000 Lishui, Zhejiang, ChinaDepartment of Anesthesia, Hangzhou Plastic Surgery Hospital, 310000 Hangzhou, Zhejiang, ChinaIntroduction: The effects of remimazolam (Re) in combination with andrographolide (AP) on learning, memory, and motor abilities in rats following cardiopulmonary bypass (CPB) surgery were studied. Methods: We hypothesized that the combination of Re and AP could improve postoperative cognitive dysfunction (POCD) in rats after CPB by modulating nervous system inflammation. Cognitive function was assessed using the Morris Water Maze test, and the concentrations of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) in serum were measured by enzyme-linked immunosorbent assay (ELISA). Apoptosis was evaluated using western blotting and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining assay. Results: The results indicated that both Re and AP independently improved cognitive function in rats after CPB and inhibited the secretion of inflammatory factors and apoptosis in hippocampal tissues. Combined administration of Re and AP enhanced the alleviation of POCD compared with monotherapy. The adenosine monophosphate-activated protein kinase/silent information regulator of transcription 1 (AMPK/SIRT1) signaling pathway was activated by the combination of Re and AP. Conclusions: Collectively, the combination of Re and AP treatment significantly improves POCD in rats after CPB through activation of the AMPK/SIRT1 signaling pathway.https://www.imrpress.com/journal/JIN/24/1/10.31083/JIN25665remimazolamandrographolidepostoperative cognitive dys-functioncardiopulmonary bypassampksirt1 |
| spellingShingle | Chong Chen Lixia Lan Kai Xu Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway Journal of Integrative Neuroscience remimazolam andrographolide postoperative cognitive dys-function cardiopulmonary bypass ampk sirt1 |
| title | Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway |
| title_full | Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway |
| title_fullStr | Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway |
| title_full_unstemmed | Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway |
| title_short | Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway |
| title_sort | remimazolam combined with andrographolide improve postoperative cognitive dysfunction in rats after cardiopulmonary bypass through the ampk sirt1 signaling pathway |
| topic | remimazolam andrographolide postoperative cognitive dys-function cardiopulmonary bypass ampk sirt1 |
| url | https://www.imrpress.com/journal/JIN/24/1/10.31083/JIN25665 |
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