Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans
Abstract Candida albicans is resistant to various antifungal drugs, this presents a significant problem on a global scale. This study investigates a novel approach on the potential fungicidal effects of α-Phellandrene combinations with fluconazole and amphotericin B against antibiotic resistant C. a...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
CABI
2024-02-01
|
| Series: | CABI Agriculture and Bioscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43170-024-00218-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573387673698304 |
|---|---|
| author | Riya Bhattacharya Prashant Sharma Debajyoti Bose Manish Singh |
| author_facet | Riya Bhattacharya Prashant Sharma Debajyoti Bose Manish Singh |
| author_sort | Riya Bhattacharya |
| collection | DOAJ |
| description | Abstract Candida albicans is resistant to various antifungal drugs, this presents a significant problem on a global scale. This study investigates a novel approach on the potential fungicidal effects of α-Phellandrene combinations with fluconazole and amphotericin B against antibiotic resistant C. albicans. The agar well diffusion experiment was used to measure the anti-candida activity of α-Phellandrene which exhibited a zone of inhibition of 24 ± 0.5 mm and 22 ± 0.5 mm against the C. albicans cells (MTCC277 and ATCC90028), respectively. Additionally, the fungicidal minimum inhibitory concentration (MIC) ranged 0.0312–0.0156 mg/ml (w/v) against C. albicans strains. It was determined to have powerful and efficient antifungal action against Candida cells. Further, the synergistic potential was evaluated by employing a time kill assay and a checkerboard technique, respectively, which revealed after 16 h, the colony count of C. albicans cells ATCC90028 (2.56 ± 0.33) and MTCC277 (2.53 ± 0.33) dropped by a log10 when treated with a combination of α-Phellandrene and Fluconazole and α-Phellandrene and amphotericin B exhibited synergy against both C. albicans strains ATCC90028 and MTCC277 (2.42 ± 0.28 and 2.00 ± 0.21) log10 reduction in colony count, respectively, Additionally, 16–624-fold increase in the antifungal efficacy of clinical medicines, with total cell death occurring after 16 h. α-Phellandrene and antifungal drugs were tested in combination with the osmoprotectant test, ergosterol test and FESEM observations to determine their modes of action. In the era of multidrug-resistant diseases antibiotic resistance can be curtailed in its tracks with the help of combination treatments that allow for lower drug doses. |
| format | Article |
| id | doaj-art-e660d4f5dd44431f9ef86d10b3368bc3 |
| institution | Kabale University |
| issn | 2662-4044 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | CABI |
| record_format | Article |
| series | CABI Agriculture and Bioscience |
| spelling | doaj-art-e660d4f5dd44431f9ef86d10b3368bc32025-02-02T04:40:24ZengCABICABI Agriculture and Bioscience2662-40442024-02-015112010.1186/s43170-024-00218-1Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicansRiya Bhattacharya0Prashant Sharma1Debajyoti Bose2Manish Singh3School of Technology, Woxsen UniversityChemical Biology Unit, Institute of Nano Science and Technology (INST)School of Technology, Woxsen UniversityChemical Biology Unit, Institute of Nano Science and Technology (INST)Abstract Candida albicans is resistant to various antifungal drugs, this presents a significant problem on a global scale. This study investigates a novel approach on the potential fungicidal effects of α-Phellandrene combinations with fluconazole and amphotericin B against antibiotic resistant C. albicans. The agar well diffusion experiment was used to measure the anti-candida activity of α-Phellandrene which exhibited a zone of inhibition of 24 ± 0.5 mm and 22 ± 0.5 mm against the C. albicans cells (MTCC277 and ATCC90028), respectively. Additionally, the fungicidal minimum inhibitory concentration (MIC) ranged 0.0312–0.0156 mg/ml (w/v) against C. albicans strains. It was determined to have powerful and efficient antifungal action against Candida cells. Further, the synergistic potential was evaluated by employing a time kill assay and a checkerboard technique, respectively, which revealed after 16 h, the colony count of C. albicans cells ATCC90028 (2.56 ± 0.33) and MTCC277 (2.53 ± 0.33) dropped by a log10 when treated with a combination of α-Phellandrene and Fluconazole and α-Phellandrene and amphotericin B exhibited synergy against both C. albicans strains ATCC90028 and MTCC277 (2.42 ± 0.28 and 2.00 ± 0.21) log10 reduction in colony count, respectively, Additionally, 16–624-fold increase in the antifungal efficacy of clinical medicines, with total cell death occurring after 16 h. α-Phellandrene and antifungal drugs were tested in combination with the osmoprotectant test, ergosterol test and FESEM observations to determine their modes of action. In the era of multidrug-resistant diseases antibiotic resistance can be curtailed in its tracks with the help of combination treatments that allow for lower drug doses.https://doi.org/10.1186/s43170-024-00218-1α-PhellandrenePhytocompoundCandida albicansSynergismAntifungal drugs |
| spellingShingle | Riya Bhattacharya Prashant Sharma Debajyoti Bose Manish Singh Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans CABI Agriculture and Bioscience α-Phellandrene Phytocompound Candida albicans Synergism Antifungal drugs |
| title | Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans |
| title_full | Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans |
| title_fullStr | Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans |
| title_full_unstemmed | Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans |
| title_short | Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans |
| title_sort | synergistic potential of α phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant candida albicans |
| topic | α-Phellandrene Phytocompound Candida albicans Synergism Antifungal drugs |
| url | https://doi.org/10.1186/s43170-024-00218-1 |
| work_keys_str_mv | AT riyabhattacharya synergisticpotentialofaphellandrenecombinedwithconventionalantifungalagentsanditsmechanismagainstantibioticresistantcandidaalbicans AT prashantsharma synergisticpotentialofaphellandrenecombinedwithconventionalantifungalagentsanditsmechanismagainstantibioticresistantcandidaalbicans AT debajyotibose synergisticpotentialofaphellandrenecombinedwithconventionalantifungalagentsanditsmechanismagainstantibioticresistantcandidaalbicans AT manishsingh synergisticpotentialofaphellandrenecombinedwithconventionalantifungalagentsanditsmechanismagainstantibioticresistantcandidaalbicans |