Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3
Abstract Apical and basal dendrites of pyramidal neurons receive anatomically and functionally distinct inputs, implying compartment-level functional diversity during behavior. To test this, we imaged in vivo calcium signals from soma, apical dendrites, and basal dendrites in mouse hippocampal CA3 p...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56289-9 |
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| author | Jason J. Moore Shannon K. Rashid Emmett Bicker Cara D. Johnson Naomi Codrington Dmitri B. Chklovskii Jayeeta Basu |
| author_facet | Jason J. Moore Shannon K. Rashid Emmett Bicker Cara D. Johnson Naomi Codrington Dmitri B. Chklovskii Jayeeta Basu |
| author_sort | Jason J. Moore |
| collection | DOAJ |
| description | Abstract Apical and basal dendrites of pyramidal neurons receive anatomically and functionally distinct inputs, implying compartment-level functional diversity during behavior. To test this, we imaged in vivo calcium signals from soma, apical dendrites, and basal dendrites in mouse hippocampal CA3 pyramidal neurons during head-fixed navigation. To capture compartment-specific population dynamics, we developed computational tools to automatically segment dendrites and extract accurate fluorescence traces from densely labeled neurons. We validated the method on sparsely labeled preparations and synthetic data, predicting an optimal labeling density for high experimental throughput and analytical accuracy. Our method detected rapid, local dendritic activity. Dendrites showed robust spatial tuning, similar to soma but with higher activity rates. Across days, apical dendrites remained more stable and outperformed in decoding of the animal’s position. Thus, population-level apical and basal dendritic differences may reflect distinct compartment-specific input-output functions and computations in CA3. These tools will facilitate future studies mapping sub-cellular activity and their relation to behavior. |
| format | Article |
| id | doaj-art-e5fa8447794e4020b4feff0caf21731f |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e5fa8447794e4020b4feff0caf21731f2025-02-02T12:33:32ZengNature PortfolioNature Communications2041-17232025-01-0116112110.1038/s41467-025-56289-9Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3Jason J. Moore0Shannon K. Rashid1Emmett Bicker2Cara D. Johnson3Naomi Codrington4Dmitri B. Chklovskii5Jayeeta Basu6Neuroscience Institute, New York University Langone HealthNeuroscience Institute, New York University Langone HealthNeuroscience Institute, New York University Langone HealthNeuroscience Institute, New York University Langone HealthNeuroscience Institute, New York University Langone HealthNeuroscience Institute, New York University Langone HealthNeuroscience Institute, New York University Langone HealthAbstract Apical and basal dendrites of pyramidal neurons receive anatomically and functionally distinct inputs, implying compartment-level functional diversity during behavior. To test this, we imaged in vivo calcium signals from soma, apical dendrites, and basal dendrites in mouse hippocampal CA3 pyramidal neurons during head-fixed navigation. To capture compartment-specific population dynamics, we developed computational tools to automatically segment dendrites and extract accurate fluorescence traces from densely labeled neurons. We validated the method on sparsely labeled preparations and synthetic data, predicting an optimal labeling density for high experimental throughput and analytical accuracy. Our method detected rapid, local dendritic activity. Dendrites showed robust spatial tuning, similar to soma but with higher activity rates. Across days, apical dendrites remained more stable and outperformed in decoding of the animal’s position. Thus, population-level apical and basal dendritic differences may reflect distinct compartment-specific input-output functions and computations in CA3. These tools will facilitate future studies mapping sub-cellular activity and their relation to behavior.https://doi.org/10.1038/s41467-025-56289-9 |
| spellingShingle | Jason J. Moore Shannon K. Rashid Emmett Bicker Cara D. Johnson Naomi Codrington Dmitri B. Chklovskii Jayeeta Basu Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3 Nature Communications |
| title | Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3 |
| title_full | Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3 |
| title_fullStr | Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3 |
| title_full_unstemmed | Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3 |
| title_short | Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3 |
| title_sort | sub cellular population imaging tools reveal stable apical dendrites in hippocampal area ca3 |
| url | https://doi.org/10.1038/s41467-025-56289-9 |
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