Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory Responses
<i>Background and Objectives</i>: Acute gastric injury is a prevalent gastrointestinal disorder characterized by inflammation and damage to the stomach lining. In this study, we investigated the therapeutic potential effects of broccoli stem extract (BSE) against acute gastritis in a rat...
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2025-01-01
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| author | Jihye Choi Yuseong Jang Hyeon-Gi Paik Melissa Hyun-Joo Ha Jungkee Kwon |
| author_facet | Jihye Choi Yuseong Jang Hyeon-Gi Paik Melissa Hyun-Joo Ha Jungkee Kwon |
| author_sort | Jihye Choi |
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| description | <i>Background and Objectives</i>: Acute gastric injury is a prevalent gastrointestinal disorder characterized by inflammation and damage to the stomach lining. In this study, we investigated the therapeutic potential effects of broccoli stem extract (BSE) against acute gastritis in a rat model. <i>Materials and Methods</i>: The antioxidant properties of BSE were evaluated through DPPH and ABTS radical scavenging activity assays and total polyphenol content analysis. Acute gastric injury was induced using 150 mM HCl/60% EtOH, and male SD rats (6-weeks old, <i>n</i> = 6/group) were administered BSE by oral gavage at concentrations of 50, 125, and 250 mg/kg. <i>Results</i>: The BSE 250 mg/kg group exhibited significant relief of clinical signs compared to the negative control group. In addition, the BSE 250 mg/kg group showed significant improvements in gastric tissue, including macroscopic reductions in ulcer size and improved overall gastric morphology as assessed through gross examination, as well as microscopic improvements such as reduced inflammation and the restoration of mucosal integrity observed in histopathological analysis. BSE modulated NF-κB signaling, decreased inflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased PGE<sub>2</sub> levels. Pyloric ligation experiments demonstrated reduced pepsin and gastric acid secretion. Improvements in gastric emptying and gastrointestinal motility were also observed in the BSE-treated group. <i>Conclusions</i>: These findings highlight the potential of BSE as an effective therapeutic agent for acute gastritis in rats, offering significant improvements in gastric damage, inflammation, and motility. |
| format | Article |
| id | doaj-art-e57ed00da5aa49cda0cb5daa4cc72004 |
| institution | Kabale University |
| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Medicina |
| spelling | doaj-art-e57ed00da5aa49cda0cb5daa4cc720042025-01-24T13:40:33ZengMDPI AGMedicina1010-660X1648-91442025-01-016118910.3390/medicina61010089Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory ResponsesJihye Choi0Yuseong Jang1Hyeon-Gi Paik2Melissa Hyun-Joo Ha3Jungkee Kwon4Department of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University, 79 Gobong-ro, Iksan-si 54596, Jeollabuk-do, Republic of KoreaDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University, 79 Gobong-ro, Iksan-si 54596, Jeollabuk-do, Republic of KoreaDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University, 79 Gobong-ro, Iksan-si 54596, Jeollabuk-do, Republic of KoreaBroccos, Room 503, 3-3 Jongangdae-ro, 296 beon-gil, Dong-gu, Busan 48730, Republic of KoreaDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University, 79 Gobong-ro, Iksan-si 54596, Jeollabuk-do, Republic of Korea<i>Background and Objectives</i>: Acute gastric injury is a prevalent gastrointestinal disorder characterized by inflammation and damage to the stomach lining. In this study, we investigated the therapeutic potential effects of broccoli stem extract (BSE) against acute gastritis in a rat model. <i>Materials and Methods</i>: The antioxidant properties of BSE were evaluated through DPPH and ABTS radical scavenging activity assays and total polyphenol content analysis. Acute gastric injury was induced using 150 mM HCl/60% EtOH, and male SD rats (6-weeks old, <i>n</i> = 6/group) were administered BSE by oral gavage at concentrations of 50, 125, and 250 mg/kg. <i>Results</i>: The BSE 250 mg/kg group exhibited significant relief of clinical signs compared to the negative control group. In addition, the BSE 250 mg/kg group showed significant improvements in gastric tissue, including macroscopic reductions in ulcer size and improved overall gastric morphology as assessed through gross examination, as well as microscopic improvements such as reduced inflammation and the restoration of mucosal integrity observed in histopathological analysis. BSE modulated NF-κB signaling, decreased inflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased PGE<sub>2</sub> levels. Pyloric ligation experiments demonstrated reduced pepsin and gastric acid secretion. Improvements in gastric emptying and gastrointestinal motility were also observed in the BSE-treated group. <i>Conclusions</i>: These findings highlight the potential of BSE as an effective therapeutic agent for acute gastritis in rats, offering significant improvements in gastric damage, inflammation, and motility.https://www.mdpi.com/1648-9144/61/1/89gastroprotectionantioxidantsbroccoli extractcytokinesgastric motility |
| spellingShingle | Jihye Choi Yuseong Jang Hyeon-Gi Paik Melissa Hyun-Joo Ha Jungkee Kwon Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory Responses Medicina gastroprotection antioxidants broccoli extract cytokines gastric motility |
| title | Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory Responses |
| title_full | Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory Responses |
| title_fullStr | Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory Responses |
| title_full_unstemmed | Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory Responses |
| title_short | Gastroprotective Effects of Aqueous Extracts of Broccoli Stems on Acute Injury in Rats: A Comprehensive Evaluation of Gastric Function and Inflammatory Responses |
| title_sort | gastroprotective effects of aqueous extracts of broccoli stems on acute injury in rats a comprehensive evaluation of gastric function and inflammatory responses |
| topic | gastroprotection antioxidants broccoli extract cytokines gastric motility |
| url | https://www.mdpi.com/1648-9144/61/1/89 |
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