Identification of Family Specific Fingerprints in β-Lactamase Families
Beta-lactamases are a superfamily of enzymes which degrade the β-lactam class of antibiotics. They are produced endogenously by the bacterial cells, which when exposed to the β-lactam class of antibiotics inactivate them by cleaving the β-lactam ring. Based on the presence or absence of metallic lig...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/980572 |
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| author | Abhishikha Srivastava Neelja Singhal Manisha Goel Jugsharan Singh Virdi Manish Kumar |
| author_facet | Abhishikha Srivastava Neelja Singhal Manisha Goel Jugsharan Singh Virdi Manish Kumar |
| author_sort | Abhishikha Srivastava |
| collection | DOAJ |
| description | Beta-lactamases are a superfamily of enzymes which degrade the β-lactam class of antibiotics. They are produced endogenously by the bacterial cells, which when exposed to the β-lactam class of antibiotics inactivate them by cleaving the β-lactam ring. Based on the presence or absence of metallic ligand, β-lactamases have been divided into two broad functional classes. β-Lactamases are a constitutively evolving and expanding superfamily of enzymes, which could be further subdivided on the basis of presence/absence of conserved motifs. In the present study we have used the MEME/MAST suit to identify the patterns/motifs which are specific to a particular family or subfamily of β-lactamases. The family specific patterns/motifs can be also useful in recognizing and assigning newly discovered β-lactamases to one or the other family or subfamily. Cross-validation showed that the proposed method is highly sensitive and specific. We have also designed a webserver, LactFP, for this purpose. |
| format | Article |
| id | doaj-art-e524cae8467e44f798a39e739a6e1585 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e524cae8467e44f798a39e739a6e15852025-02-03T05:51:20ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/980572980572Identification of Family Specific Fingerprints in β-Lactamase FamiliesAbhishikha Srivastava0Neelja Singhal1Manisha Goel2Jugsharan Singh Virdi3Manish Kumar4Department of Biophysics, University of Delhi South Campus, New Delhi 110 021, IndiaDepartment of Microbiology, University of Delhi South Campus, New Delhi 110 021, IndiaDepartment of Biophysics, University of Delhi South Campus, New Delhi 110 021, IndiaDepartment of Microbiology, University of Delhi South Campus, New Delhi 110 021, IndiaDepartment of Biophysics, University of Delhi South Campus, New Delhi 110 021, IndiaBeta-lactamases are a superfamily of enzymes which degrade the β-lactam class of antibiotics. They are produced endogenously by the bacterial cells, which when exposed to the β-lactam class of antibiotics inactivate them by cleaving the β-lactam ring. Based on the presence or absence of metallic ligand, β-lactamases have been divided into two broad functional classes. β-Lactamases are a constitutively evolving and expanding superfamily of enzymes, which could be further subdivided on the basis of presence/absence of conserved motifs. In the present study we have used the MEME/MAST suit to identify the patterns/motifs which are specific to a particular family or subfamily of β-lactamases. The family specific patterns/motifs can be also useful in recognizing and assigning newly discovered β-lactamases to one or the other family or subfamily. Cross-validation showed that the proposed method is highly sensitive and specific. We have also designed a webserver, LactFP, for this purpose.http://dx.doi.org/10.1155/2014/980572 |
| spellingShingle | Abhishikha Srivastava Neelja Singhal Manisha Goel Jugsharan Singh Virdi Manish Kumar Identification of Family Specific Fingerprints in β-Lactamase Families The Scientific World Journal |
| title | Identification of Family Specific Fingerprints in β-Lactamase Families |
| title_full | Identification of Family Specific Fingerprints in β-Lactamase Families |
| title_fullStr | Identification of Family Specific Fingerprints in β-Lactamase Families |
| title_full_unstemmed | Identification of Family Specific Fingerprints in β-Lactamase Families |
| title_short | Identification of Family Specific Fingerprints in β-Lactamase Families |
| title_sort | identification of family specific fingerprints in β lactamase families |
| url | http://dx.doi.org/10.1155/2014/980572 |
| work_keys_str_mv | AT abhishikhasrivastava identificationoffamilyspecificfingerprintsinblactamasefamilies AT neeljasinghal identificationoffamilyspecificfingerprintsinblactamasefamilies AT manishagoel identificationoffamilyspecificfingerprintsinblactamasefamilies AT jugsharansinghvirdi identificationoffamilyspecificfingerprintsinblactamasefamilies AT manishkumar identificationoffamilyspecificfingerprintsinblactamasefamilies |