Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> Sporophore
<i>Lentinus tigrinus</i> is a wood-decay fungus known for its nutritional, culinary, and medicinal benefits. It contains bioactive compounds like polyphenols, terpenes, and flavonoids that exhibit antioxidant, anti-microbial, and anti-inflammatory effects. These natural antioxidants are...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/2077-0472/15/2/178 |
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| author | Anthea Desiderio Mariana C. Pedrosa Sandrina A. Heleno Marcio Carocho Daniele B. Rodrigues Simone Buratti Irene Soffientini Daniela Ratto Elena Savino Paola Rossi |
| author_facet | Anthea Desiderio Mariana C. Pedrosa Sandrina A. Heleno Marcio Carocho Daniele B. Rodrigues Simone Buratti Irene Soffientini Daniela Ratto Elena Savino Paola Rossi |
| author_sort | Anthea Desiderio |
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| description | <i>Lentinus tigrinus</i> is a wood-decay fungus known for its nutritional, culinary, and medicinal benefits. It contains bioactive compounds like polyphenols, terpenes, and flavonoids that exhibit antioxidant, anti-microbial, and anti-inflammatory effects. These natural antioxidants are increasingly recognized for their potential to prevent oxidative damage linked to aging and chronic diseases. This study investigates the antioxidant activity of hydroalcoholic extracts obtained from <i>L. tigrinus</i> sporophores cultivated on hazelnut shells (Lt1_HS), a waste material rich in phenolic compounds, and sporophores cultivated on sawdust (Lt1_S). Bioactivity tests, including DPPH, TBARS, MTT, and DCFH-DA assays, were performed to assess the hydroalcoholic extracts’ efficiency. The results showed that all the extracts contained various bioactive compounds, primarily polyphenols. Notably, the caffeoylquinic acids present in HS and Lt1_HS are linked to anti-peroxidant effects. Biological analyses demonstrated that the Lt1_HS extract has higher anti-peroxidant activity (IC<sub>50</sub> 0.77 ± 0.01 mg/mL) compared to Lt1_S (IC<sub>50</sub> 1.36 ± 0.01 mg/mL) and reduces the accumulation of reactive oxygen species in HaCaT cells by 80%. However, the specific bioactive compounds responsible for these antioxidant effects are still unclear, and further analysis will be conducted. Additionally, this study promotes recycling hazelnut shells as a valuable substrate for fungal cultivation, supporting sustainable waste management. |
| format | Article |
| id | doaj-art-e3ec88c6ab0b4ccdbb19ec48b924d13e |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-e3ec88c6ab0b4ccdbb19ec48b924d13e2025-01-24T13:16:01ZengMDPI AGAgriculture2077-04722025-01-0115217810.3390/agriculture15020178Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> SporophoreAnthea Desiderio0Mariana C. Pedrosa1Sandrina A. Heleno2Marcio Carocho3Daniele B. Rodrigues4Simone Buratti5Irene Soffientini6Daniela Ratto7Elena Savino8Paola Rossi9Department of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyCIMO—Centro de Invesigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCIMO—Centro de Invesigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCIMO—Centro de Invesigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCIMO—Centro de Invesigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalDepartment of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, ItalyDepartment of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, Italy<i>Lentinus tigrinus</i> is a wood-decay fungus known for its nutritional, culinary, and medicinal benefits. It contains bioactive compounds like polyphenols, terpenes, and flavonoids that exhibit antioxidant, anti-microbial, and anti-inflammatory effects. These natural antioxidants are increasingly recognized for their potential to prevent oxidative damage linked to aging and chronic diseases. This study investigates the antioxidant activity of hydroalcoholic extracts obtained from <i>L. tigrinus</i> sporophores cultivated on hazelnut shells (Lt1_HS), a waste material rich in phenolic compounds, and sporophores cultivated on sawdust (Lt1_S). Bioactivity tests, including DPPH, TBARS, MTT, and DCFH-DA assays, were performed to assess the hydroalcoholic extracts’ efficiency. The results showed that all the extracts contained various bioactive compounds, primarily polyphenols. Notably, the caffeoylquinic acids present in HS and Lt1_HS are linked to anti-peroxidant effects. Biological analyses demonstrated that the Lt1_HS extract has higher anti-peroxidant activity (IC<sub>50</sub> 0.77 ± 0.01 mg/mL) compared to Lt1_S (IC<sub>50</sub> 1.36 ± 0.01 mg/mL) and reduces the accumulation of reactive oxygen species in HaCaT cells by 80%. However, the specific bioactive compounds responsible for these antioxidant effects are still unclear, and further analysis will be conducted. Additionally, this study promotes recycling hazelnut shells as a valuable substrate for fungal cultivation, supporting sustainable waste management.https://www.mdpi.com/2077-0472/15/2/178medicinal mushroomsbiological effectscultivationwaste substratebioactive compounds |
| spellingShingle | Anthea Desiderio Mariana C. Pedrosa Sandrina A. Heleno Marcio Carocho Daniele B. Rodrigues Simone Buratti Irene Soffientini Daniela Ratto Elena Savino Paola Rossi Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> Sporophore Agriculture medicinal mushrooms biological effects cultivation waste substrate bioactive compounds |
| title | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> Sporophore |
| title_full | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> Sporophore |
| title_fullStr | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> Sporophore |
| title_full_unstemmed | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> Sporophore |
| title_short | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of <i>Lentinus tigrinus</i> Sporophore |
| title_sort | bio recycling hazelnut shells to improve antioxidant properties of i lentinus tigrinus i sporophore |
| topic | medicinal mushrooms biological effects cultivation waste substrate bioactive compounds |
| url | https://www.mdpi.com/2077-0472/15/2/178 |
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