Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure
Protease inhibitors trypsin (STI1, Streptomyces trypsin inhibitor 1) has been identified, purified by ammonium sulfate precipitation and Sephadex G-100 gel filtration. SDS-PAGE of protease inhibitor showed molecular weight of approximately 10 KDa. PCR product (~1615 bp) of sti1 gene was cloned in e...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/963041 |
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| author | D. E. El-Hadedy El-Sayed E. Mostafa Moataza M. Saad |
| author_facet | D. E. El-Hadedy El-Sayed E. Mostafa Moataza M. Saad |
| author_sort | D. E. El-Hadedy |
| collection | DOAJ |
| description | Protease inhibitors trypsin (STI1, Streptomyces trypsin inhibitor 1) has been identified, purified by ammonium sulfate precipitation and Sephadex G-100 gel filtration. SDS-PAGE of protease inhibitor showed molecular weight of approximately 10 KDa. PCR product (~1615 bp) of sti1 gene was cloned in expression vector pACYC177/ET3d and transformed in Escherichia coli JM109. Protease inhibitors trypsin was purified and used as antivirus against Coxsackievirus B3 (CVB3). CVB3 is one of the major causative agents of chronic, subacute, acute, and fulminant myocarditis as well as pancreatitis and aseptic meningitis. It has been reported that more than 50% of human myocarditis is associated with CVB3 infection. |
| format | Article |
| id | doaj-art-e2ecc22417a2496db0426989af8d6bb7 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-e2ecc22417a2496db0426989af8d6bb72025-02-03T05:45:24ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/963041963041Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional StructureD. E. El-Hadedy0El-Sayed E. Mostafa1Moataza M. Saad2Microbiology Department, National Center for Radiation Research and Technology (NCRRT), 3 Ahmed El-Zomor Street, 8th Sector, Nasr City, Cairo 11371, EgyptMicrobial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, P.O. Box 12622, Dokki, Cairo, EgyptMicrobial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, P.O. Box 12622, Dokki, Cairo, EgyptProtease inhibitors trypsin (STI1, Streptomyces trypsin inhibitor 1) has been identified, purified by ammonium sulfate precipitation and Sephadex G-100 gel filtration. SDS-PAGE of protease inhibitor showed molecular weight of approximately 10 KDa. PCR product (~1615 bp) of sti1 gene was cloned in expression vector pACYC177/ET3d and transformed in Escherichia coli JM109. Protease inhibitors trypsin was purified and used as antivirus against Coxsackievirus B3 (CVB3). CVB3 is one of the major causative agents of chronic, subacute, acute, and fulminant myocarditis as well as pancreatitis and aseptic meningitis. It has been reported that more than 50% of human myocarditis is associated with CVB3 infection.http://dx.doi.org/10.1155/2015/963041 |
| spellingShingle | D. E. El-Hadedy El-Sayed E. Mostafa Moataza M. Saad Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure Journal of Chemistry |
| title | Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure |
| title_full | Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure |
| title_fullStr | Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure |
| title_full_unstemmed | Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure |
| title_short | Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure |
| title_sort | streptomyces lavendulae protease inhibitor purification gene overexpression and 3 dimensional structure |
| url | http://dx.doi.org/10.1155/2015/963041 |
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