Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest
Abstract Bladder cancer often recurs, necessitating innovative treatments to reduce recurrence. We investigated non-thermal plasma’s potential as a novel anti-cancer therapy, focusing on plasma-activated solution (PAS), created by exposing saline to non-thermal plasma. Our study aims to elucidate th...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-85568-0 |
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| author | Jojanneke Stoof Zakaria Kalmoua Ana Sobota Ruud H. Brakenhoff Marijke Stigter Thang V. Pham Sander R. Piersma Alex Henneman Tonny Lagerweij Richard de Goeij-de Haas R. Jeroen A. van Moorselaar Connie R. Jimenez Irene V. Bijnsdorp |
| author_facet | Jojanneke Stoof Zakaria Kalmoua Ana Sobota Ruud H. Brakenhoff Marijke Stigter Thang V. Pham Sander R. Piersma Alex Henneman Tonny Lagerweij Richard de Goeij-de Haas R. Jeroen A. van Moorselaar Connie R. Jimenez Irene V. Bijnsdorp |
| author_sort | Jojanneke Stoof |
| collection | DOAJ |
| description | Abstract Bladder cancer often recurs, necessitating innovative treatments to reduce recurrence. We investigated non-thermal plasma’s potential as a novel anti-cancer therapy, focusing on plasma-activated solution (PAS), created by exposing saline to non-thermal plasma. Our study aims to elucidate the biological effects of PAS on bladder cancer cell lines in vitro, as well as the combination with mitomycin C (MMC), using clinically relevant settings. PAS treatment exerts a potent cytotoxic effect through the production of intracellular reactive oxygen species, resulting in DNA damage and subsequent induction of G1 cell cycle arrest/senescence. This is induced via upregulation of cell cycle checkpoint signalling and DNA damage repair pathways using LC-M/MS-based phospho-proteomics. Importantly, combining PAS with MMC reveals a synergistic effect (Combination Index of 0.59–0.67), suggesting the potential of utilizing PAS in combination therapies. Our findings demonstrate PAS’s mode of action and suggest its potential as a promising treatment for bladder cancer, warranting further clinical studies. |
| format | Article |
| id | doaj-art-e2b877d8a49345e78dd1b908153d5aa8 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e2b877d8a49345e78dd1b908153d5aa82025-01-19T12:19:18ZengNature PortfolioScientific Reports2045-23222025-01-0115111010.1038/s41598-025-85568-0Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrestJojanneke Stoof0Zakaria Kalmoua1Ana Sobota2Ruud H. Brakenhoff3Marijke Stigter4Thang V. Pham5Sander R. Piersma6Alex Henneman7Tonny Lagerweij8Richard de Goeij-de Haas9R. Jeroen A. van Moorselaar10Connie R. Jimenez11Irene V. Bijnsdorp12Amsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamDepartment of Applied Physics and Science Education, Eindhoven University of TechnologyAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAmsterdam UMC, Location VUmc, Cancer Center AmsterdamAbstract Bladder cancer often recurs, necessitating innovative treatments to reduce recurrence. We investigated non-thermal plasma’s potential as a novel anti-cancer therapy, focusing on plasma-activated solution (PAS), created by exposing saline to non-thermal plasma. Our study aims to elucidate the biological effects of PAS on bladder cancer cell lines in vitro, as well as the combination with mitomycin C (MMC), using clinically relevant settings. PAS treatment exerts a potent cytotoxic effect through the production of intracellular reactive oxygen species, resulting in DNA damage and subsequent induction of G1 cell cycle arrest/senescence. This is induced via upregulation of cell cycle checkpoint signalling and DNA damage repair pathways using LC-M/MS-based phospho-proteomics. Importantly, combining PAS with MMC reveals a synergistic effect (Combination Index of 0.59–0.67), suggesting the potential of utilizing PAS in combination therapies. Our findings demonstrate PAS’s mode of action and suggest its potential as a promising treatment for bladder cancer, warranting further clinical studies.https://doi.org/10.1038/s41598-025-85568-0Non-thermal plasmaPhospho-proteomicsBladder cancerCancer therapyPlasma oncology |
| spellingShingle | Jojanneke Stoof Zakaria Kalmoua Ana Sobota Ruud H. Brakenhoff Marijke Stigter Thang V. Pham Sander R. Piersma Alex Henneman Tonny Lagerweij Richard de Goeij-de Haas R. Jeroen A. van Moorselaar Connie R. Jimenez Irene V. Bijnsdorp Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest Scientific Reports Non-thermal plasma Phospho-proteomics Bladder cancer Cancer therapy Plasma oncology |
| title | Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest |
| title_full | Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest |
| title_fullStr | Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest |
| title_full_unstemmed | Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest |
| title_short | Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest |
| title_sort | non thermal plasma as promising anti cancer therapy against bladder cancer by inducing dna damage and cell cycle arrest |
| topic | Non-thermal plasma Phospho-proteomics Bladder cancer Cancer therapy Plasma oncology |
| url | https://doi.org/10.1038/s41598-025-85568-0 |
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