Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis
The gut microbiome is crucial in regulating nutrient metabolism, immune responses, and defense mechanisms against diseases. This study aimed to comprehensively compare bacterial community profiles within the foregut, midgut, and hindgut regions of red tilapia fish with and without streptococcosis. R...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006446 |
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| author | Pathamawan Amthungphong Natthaphong Sombuttra Triwit Rattanarojpong Kanthida Kusonmano Saengchan Senapin Satid Chatchaiphan Watanalai Panbangred Jirayut Euanorasetr |
| author_facet | Pathamawan Amthungphong Natthaphong Sombuttra Triwit Rattanarojpong Kanthida Kusonmano Saengchan Senapin Satid Chatchaiphan Watanalai Panbangred Jirayut Euanorasetr |
| author_sort | Pathamawan Amthungphong |
| collection | DOAJ |
| description | The gut microbiome is crucial in regulating nutrient metabolism, immune responses, and defense mechanisms against diseases. This study aimed to comprehensively compare bacterial community profiles within the foregut, midgut, and hindgut regions of red tilapia fish with and without streptococcosis. Red tilapia (Oreochromis niloticus × Oreochromis mossambicus) with and without streptococcosis were collected from a river cage culture farm in Central Thailand. The infected streptococcal isolates were confirmed by 16S rRNA sequencing. The 16S amplicon was sequenced in gut microbiome samples from healthy and diseased groups to analyze the bacterial composition profiles associated with red tilapia with and without streptococcosis. The alpha diversity of bacterial communities in the gut microbiome was significantly higher in the healthy group than in the diseased group. Beta diversity indicated distinct differences in bacterial communities between groups. Taxonomical profiling revealed Proteobacteria followed by Fusobacteria as the dominant phyla and Cetobacterium as the dominant genus in the gut of both groups. Linear discriminant analysis effect size indicated Vibrio, Plesiomonas, Aeromonas, Streptococcus, Chitinibacter, and Edwardsiella as relatively more abundant in the diseased group. These groups could serve as baseline populations associated with streptococcosis in red tilapia. In contrast, Romboutsia, Clostridium sensu stricto 1, Turicibacter, Epulopiscium, Bacillus, Streptomyces, Hyphomicrobium, and Nocardioides were more abundant in the healthy group. This study provides insights into the bacterial community profiles associated with streptococcosis in the gut of red tilapia. It enhances our understanding of gut bacterial communities in red tilapia and offers a baseline perspective on bacterial populations associated with streptococcosis, which could benefit future treatment and diagnostic strategies. |
| format | Article |
| id | doaj-art-e20531fa3d364f4c851dd86865725621 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-e20531fa3d364f4c851dd868657256212025-02-06T05:12:07ZengElsevierAquaculture Reports2352-51342025-03-0140102556Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosisPathamawan Amthungphong0Natthaphong Sombuttra1Triwit Rattanarojpong2Kanthida Kusonmano3Saengchan Senapin4Satid Chatchaiphan5Watanalai Panbangred6Jirayut Euanorasetr7Laboratory of Biotechnological Research for Energy and Bioactive Compound, Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, ThailandLaboratory of Biotechnological Research for Energy and Bioactive Compound, Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, ThailandDepartment of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, ThailandBioinformatics and Systems Biology Program, Schools of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand; Systems Biology and Bioinformatics Research Group, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok 10150, ThailandCenter of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, ThailandDepartment of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, ThailandResearch, Innovation and Partnerships Office, King Mongkut’s University of Technology Thonburi, Bangkok 10140, ThailandLaboratory of Biotechnological Research for Energy and Bioactive Compound, Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand; Corresponding author.The gut microbiome is crucial in regulating nutrient metabolism, immune responses, and defense mechanisms against diseases. This study aimed to comprehensively compare bacterial community profiles within the foregut, midgut, and hindgut regions of red tilapia fish with and without streptococcosis. Red tilapia (Oreochromis niloticus × Oreochromis mossambicus) with and without streptococcosis were collected from a river cage culture farm in Central Thailand. The infected streptococcal isolates were confirmed by 16S rRNA sequencing. The 16S amplicon was sequenced in gut microbiome samples from healthy and diseased groups to analyze the bacterial composition profiles associated with red tilapia with and without streptococcosis. The alpha diversity of bacterial communities in the gut microbiome was significantly higher in the healthy group than in the diseased group. Beta diversity indicated distinct differences in bacterial communities between groups. Taxonomical profiling revealed Proteobacteria followed by Fusobacteria as the dominant phyla and Cetobacterium as the dominant genus in the gut of both groups. Linear discriminant analysis effect size indicated Vibrio, Plesiomonas, Aeromonas, Streptococcus, Chitinibacter, and Edwardsiella as relatively more abundant in the diseased group. These groups could serve as baseline populations associated with streptococcosis in red tilapia. In contrast, Romboutsia, Clostridium sensu stricto 1, Turicibacter, Epulopiscium, Bacillus, Streptomyces, Hyphomicrobium, and Nocardioides were more abundant in the healthy group. This study provides insights into the bacterial community profiles associated with streptococcosis in the gut of red tilapia. It enhances our understanding of gut bacterial communities in red tilapia and offers a baseline perspective on bacterial populations associated with streptococcosis, which could benefit future treatment and diagnostic strategies.http://www.sciencedirect.com/science/article/pii/S2352513424006446Red tilapiaStreptococcosisGut microbiomeBacterial community profile |
| spellingShingle | Pathamawan Amthungphong Natthaphong Sombuttra Triwit Rattanarojpong Kanthida Kusonmano Saengchan Senapin Satid Chatchaiphan Watanalai Panbangred Jirayut Euanorasetr Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis Aquaculture Reports Red tilapia Streptococcosis Gut microbiome Bacterial community profile |
| title | Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis |
| title_full | Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis |
| title_fullStr | Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis |
| title_full_unstemmed | Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis |
| title_short | Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis |
| title_sort | comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis |
| topic | Red tilapia Streptococcosis Gut microbiome Bacterial community profile |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006446 |
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