Molecular investigation of Hepatozoon canis in dogs in Mosul city
This study is the first molecular detection of Hepatozoon canis in dogs in Mosul city. One hundred twenty blood samples were collected from stray and household dogs of different ages, sexes, breeds, and breeding, suffering from emaciation, anemia, paleness in mucous membranes and the presence of tic...
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| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_186155_730dc4a7888bd4b86224a31e76a20396.pdf |
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| Summary: | This study is the first molecular detection of Hepatozoon canis in dogs in Mosul city. One hundred twenty blood samples were collected from stray and household dogs of different ages, sexes, breeds, and breeding, suffering from emaciation, anemia, paleness in mucous membranes and the presence of ticks in the ear. The DNA was extracted using a commercial kit (Qiagen, Germany). Polymerase Chain Reaction was used to detect the parasite targeting a partial region of 18S rRNA gene. The polymerase chain reaction produced positive results, as bands in electrophoresis at 737 bp. The total positive sample was 33 dogs out of 120 dogs (27.5%) the relationship between the infection with the parasite and the age of animals recorded a high prevalence of infection in dogs less than 6 months while the lowest prevalence of infection in animals more than 2 years old, The male dogs showed a high prevalence of infection when compared with female dog, The large breeds dogs recorded high prevalence of infection than small breeds dogs, The outdoor dogs showed a high prevalence of infection Animals which suffer from paleness in mucous membrane showed high prevalence, while healthy animals recorded lowest prevalence of infection, gene sequence showed there were only three isolates out of the total samples sent for genetic sequencing. |
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| ISSN: | 1607-3894 2071-1255 |