Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc
The clinical application of human bone marrow mesenchymal stem cells (hBM-MSCs) has generated a great deal of interest because of their potential use in regenerative medicine and tissue engineering. However, safety concerns over hBM-MSCs limit their clinical application. In this study, we observed t...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/9501747 |
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| _version_ | 1832563323499970560 |
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| author | Bin Chen Jing Yu Qianqian Wang Yuanyuan Zhao Li Sun Changgen Xu Xiangdong Zhao Bo Shen Mei Wang Wenrong Xu Wei Zhu |
| author_facet | Bin Chen Jing Yu Qianqian Wang Yuanyuan Zhao Li Sun Changgen Xu Xiangdong Zhao Bo Shen Mei Wang Wenrong Xu Wei Zhu |
| author_sort | Bin Chen |
| collection | DOAJ |
| description | The clinical application of human bone marrow mesenchymal stem cells (hBM-MSCs) has generated a great deal of interest because of their potential use in regenerative medicine and tissue engineering. However, safety concerns over hBM-MSCs limit their clinical application. In this study, we observed that hBM-MSC-conditioned medium (hBM-MSC-CM) promotes gastric cancer development via upregulation of c-Myc. Our results showed that c-Myc was upregulated in MGC-803 and BGC-823 cells after hBM-MSC-CM treatment. Moreover, we found that the c-Myc inhibitor JQ1 and c-Myc siRNA decreased the expression of c-Myc in hBM-MSC-CM-treated tumor cells in vitro. Additionally, hBM-MSC-CM enhanced the migration and glucose uptake of gastric cancer cells. In vivo studies showed that JQ1 inhibited hBM-MSC-CM-induced gastric cancer growth. These results indicated that hBM-MSC-CM induced gastric cancer growth via upregulation of c-Myc, which may be a potential risk factor and/or a therapeutic target for clinical applications. |
| format | Article |
| id | doaj-art-e16f1d4d40a349aca79eccf8be1ac938 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e16f1d4d40a349aca79eccf8be1ac9382025-02-03T01:20:33ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/95017479501747Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-MycBin Chen0Jing Yu1Qianqian Wang2Yuanyuan Zhao3Li Sun4Changgen Xu5Xiangdong Zhao6Bo Shen7Mei Wang8Wenrong Xu9Wei Zhu10School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaZhenjiang Provincial Blood Center, Zhenjiang, Jiangsu, ChinaZhenjiang Provincial Blood Center, Zhenjiang, Jiangsu, ChinaDepartment of Oncology, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaThe clinical application of human bone marrow mesenchymal stem cells (hBM-MSCs) has generated a great deal of interest because of their potential use in regenerative medicine and tissue engineering. However, safety concerns over hBM-MSCs limit their clinical application. In this study, we observed that hBM-MSC-conditioned medium (hBM-MSC-CM) promotes gastric cancer development via upregulation of c-Myc. Our results showed that c-Myc was upregulated in MGC-803 and BGC-823 cells after hBM-MSC-CM treatment. Moreover, we found that the c-Myc inhibitor JQ1 and c-Myc siRNA decreased the expression of c-Myc in hBM-MSC-CM-treated tumor cells in vitro. Additionally, hBM-MSC-CM enhanced the migration and glucose uptake of gastric cancer cells. In vivo studies showed that JQ1 inhibited hBM-MSC-CM-induced gastric cancer growth. These results indicated that hBM-MSC-CM induced gastric cancer growth via upregulation of c-Myc, which may be a potential risk factor and/or a therapeutic target for clinical applications.http://dx.doi.org/10.1155/2018/9501747 |
| spellingShingle | Bin Chen Jing Yu Qianqian Wang Yuanyuan Zhao Li Sun Changgen Xu Xiangdong Zhao Bo Shen Mei Wang Wenrong Xu Wei Zhu Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc Stem Cells International |
| title | Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc |
| title_full | Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc |
| title_fullStr | Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc |
| title_full_unstemmed | Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc |
| title_short | Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc |
| title_sort | human bone marrow mesenchymal stem cells promote gastric cancer growth via regulating c myc |
| url | http://dx.doi.org/10.1155/2018/9501747 |
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