Effects of a high-fat diet on the lipid profile of oocytes in mice
There are evidences that obese women exhibit a detrimental oocyte quality. However, it remains unclear how this change is associated with obesity, indirectly – or directly through a change in the content and/or composition of lipids in oocytes. The aim of this work was to study effects of a high-fat...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2020-08-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2718 |
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| author | E. Yu. Brusentsev E. A. Chuyko K. A. Okotrub T. N. Igonina I. N. Rozhkova D. S. Ragaeva S. V. Ranneva V. A. Naprimerov S. Ya. Amstislavsky |
| author_facet | E. Yu. Brusentsev E. A. Chuyko K. A. Okotrub T. N. Igonina I. N. Rozhkova D. S. Ragaeva S. V. Ranneva V. A. Naprimerov S. Ya. Amstislavsky |
| author_sort | E. Yu. Brusentsev |
| collection | DOAJ |
| description | There are evidences that obese women exhibit a detrimental oocyte quality. However, it remains unclear how this change is associated with obesity, indirectly – or directly through a change in the content and/or composition of lipids in oocytes. The aim of this work was to study effects of a high-fat diet applied to female donor mice on the amount and qualitative composition of lipids of immature and in vivo matured oocytes. A high-fat diet caused larger body weight in female mice compared with the control (p < 0.001; 44.77±1.46 and 35.22±1.57, respectively), and increased the blood levels of cholesterol (p < 0.05; 2.06±0.10 and 1.78±0.10, respectively) and triglycerides (p < 0.05; 2.13±0.23 and 1.49±0.21, respectively). At the same time, this diet does not affect the level of unsaturation of lipids in immature (0.207±0.004 in the experiment and 0.206±0.002 in the control) and matured oocytes (0.212±0.005 in the experiment and 0.211±0.003 in the control). Total lipid content increased during in vivo maturation of mouse oocytes. The amount of lipids was greater in mature oocytes in the experimental group compared to the control (p < 0.01; 8.15±0.37 and 5.83±0.14, respectively). An increase in intracellular lipid amount during oocyte maturation was revealed both after a standard diet (p < 0.05; 4.72±0.48 and 5.83±0.14, respectively) and after a fat-rich diet (p < 0.001; 3.45±0.62 and 8.15±0.37, respectively). Thus, during in vivo oocyte maturation in mice the content of intracellular lipids enhanced, the high-fat diet aggravated this dynamics of lipid increase during in vivo maturation of oocytes. |
| format | Article |
| id | doaj-art-e11c4136fda840fcbf91cb2fa8c5962f |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2020-08-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-e11c4136fda840fcbf91cb2fa8c5962f2025-02-01T09:58:09ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592020-08-0124553353810.18699/VJ20.6451080Effects of a high-fat diet on the lipid profile of oocytes in miceE. Yu. Brusentsev0E. A. Chuyko1K. A. Okotrub2T. N. Igonina3I. N. Rozhkova4D. S. Ragaeva5S. V. Ranneva6V. A. Naprimerov7S. Ya. Amstislavsky8Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityInstitute of Automation and Electrometry of Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences; Novosibirsk State Agrarian UniversityInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityThere are evidences that obese women exhibit a detrimental oocyte quality. However, it remains unclear how this change is associated with obesity, indirectly – or directly through a change in the content and/or composition of lipids in oocytes. The aim of this work was to study effects of a high-fat diet applied to female donor mice on the amount and qualitative composition of lipids of immature and in vivo matured oocytes. A high-fat diet caused larger body weight in female mice compared with the control (p < 0.001; 44.77±1.46 and 35.22±1.57, respectively), and increased the blood levels of cholesterol (p < 0.05; 2.06±0.10 and 1.78±0.10, respectively) and triglycerides (p < 0.05; 2.13±0.23 and 1.49±0.21, respectively). At the same time, this diet does not affect the level of unsaturation of lipids in immature (0.207±0.004 in the experiment and 0.206±0.002 in the control) and matured oocytes (0.212±0.005 in the experiment and 0.211±0.003 in the control). Total lipid content increased during in vivo maturation of mouse oocytes. The amount of lipids was greater in mature oocytes in the experimental group compared to the control (p < 0.01; 8.15±0.37 and 5.83±0.14, respectively). An increase in intracellular lipid amount during oocyte maturation was revealed both after a standard diet (p < 0.05; 4.72±0.48 and 5.83±0.14, respectively) and after a fat-rich diet (p < 0.001; 3.45±0.62 and 8.15±0.37, respectively). Thus, during in vivo oocyte maturation in mice the content of intracellular lipids enhanced, the high-fat diet aggravated this dynamics of lipid increase during in vivo maturation of oocytes.https://vavilov.elpub.ru/jour/article/view/2718micedietoocytesintracellular lipidsnile redconfocal laser scanning microscopyraman spectroscopy |
| spellingShingle | E. Yu. Brusentsev E. A. Chuyko K. A. Okotrub T. N. Igonina I. N. Rozhkova D. S. Ragaeva S. V. Ranneva V. A. Naprimerov S. Ya. Amstislavsky Effects of a high-fat diet on the lipid profile of oocytes in mice Вавиловский журнал генетики и селекции mice diet oocytes intracellular lipids nile red confocal laser scanning microscopy raman spectroscopy |
| title | Effects of a high-fat diet on the lipid profile of oocytes in mice |
| title_full | Effects of a high-fat diet on the lipid profile of oocytes in mice |
| title_fullStr | Effects of a high-fat diet on the lipid profile of oocytes in mice |
| title_full_unstemmed | Effects of a high-fat diet on the lipid profile of oocytes in mice |
| title_short | Effects of a high-fat diet on the lipid profile of oocytes in mice |
| title_sort | effects of a high fat diet on the lipid profile of oocytes in mice |
| topic | mice diet oocytes intracellular lipids nile red confocal laser scanning microscopy raman spectroscopy |
| url | https://vavilov.elpub.ru/jour/article/view/2718 |
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