Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes
In spite of the global barcoding effort, there is still a lack of genetic data on African freshwater fishes. We aimed to contribute to bridging this gap by providing molecular data on commercially important catfish species from the north-western part of Lake Tanganyika. We collected 215 catfish spec...
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2024-12-01
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| author | Archimède Mushagalusa Mulega Arthur F. Boom Imane Rahmouni Hocein Bazairi Abdelaziz Benhoussa Maarten P. M. Vanhove Maarten Van Steenberge |
| author_facet | Archimède Mushagalusa Mulega Arthur F. Boom Imane Rahmouni Hocein Bazairi Abdelaziz Benhoussa Maarten P. M. Vanhove Maarten Van Steenberge |
| author_sort | Archimède Mushagalusa Mulega |
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| description | In spite of the global barcoding effort, there is still a lack of genetic data on African freshwater fishes. We aimed to contribute to bridging this gap by providing molecular data on commercially important catfish species from the north-western part of Lake Tanganyika. We collected 215 catfish specimens and sequenced the standard vertebrate barcoding gene (COI) for 41 specimens. Additionally, we sequenced 20 specimens for the mitochondrial Cyt-b gene to make the link to previously published datasets. We identified 11 species using morphology, compared DNA sequences with those available on GenBank, and employed Automatic Barcode Gap Discovery (ABGD) and phylogenetic approaches. The dataset includes the first molecular data (COI and Cyt-b) for <i>Chrysichthys acsiorum</i>, as well as the first-ever COI sequences for <i>Dinotopterus cunningtoni</i> and <i>Malapterurus tanganyikaensis</i>. Our findings extend the known distribution of <i>C. acsiorum</i> by approximately 100 km. Additionally, we demonstrated the difficulty in delineating species of <i>Chrysichthys</i> and <i>Synodontis</i> from Lake Tanganyika with molecular tools. For <i>Chrysichthys</i>, automated methods, such as ABGD, failed to delineate species. However, barcoding does seem promising as all the individual species are resolved as clades. Within <i>Synodontis</i>, the study found a strong similarity between <i>S. grandiops</i> and <i>S. multipunctatus</i>, highlighting a need for revision. Our findings emphasize the necessity for integrative taxonomy in the study of catfishes from Lake Tanganyika. |
| format | Article |
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| institution | Kabale University |
| issn | 1424-2818 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Diversity |
| spelling | doaj-art-e119dc3b17f3408b830fc9a7fb4320c52025-01-24T13:29:19ZengMDPI AGDiversity1424-28182024-12-01171810.3390/d17010008Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA BarcodesArchimède Mushagalusa Mulega0Arthur F. Boom1Imane Rahmouni2Hocein Bazairi3Abdelaziz Benhoussa4Maarten P. M. Vanhove5Maarten Van Steenberge6Laboratory Biodiversity, Ecology and Genome, Research Center Plant and Microbial Biotechnology, Biodiversity and Environment, Mohammed V University in Rabat, Rabat 10100, MoroccoRoyal Museum for Central Africa, Biology Department, Section Vertebrates, 3080 Tervuren, BelgiumLaboratory Biodiversity, Ecology and Genome, Research Center Plant and Microbial Biotechnology, Biodiversity and Environment, Mohammed V University in Rabat, Rabat 10100, MoroccoLaboratory Biodiversity, Ecology and Genome, Research Center Plant and Microbial Biotechnology, Biodiversity and Environment, Mohammed V University in Rabat, Rabat 10100, MoroccoLaboratory Biodiversity, Ecology and Genome, Research Center Plant and Microbial Biotechnology, Biodiversity and Environment, Mohammed V University in Rabat, Rabat 10100, MoroccoResearch Group Zoology: Biodiversity & Toxicology, Centre for Environmental Sciences, Hasselt University, 3590 Diepenbeek, BelgiumResearch Group Zoology: Biodiversity & Toxicology, Centre for Environmental Sciences, Hasselt University, 3590 Diepenbeek, BelgiumIn spite of the global barcoding effort, there is still a lack of genetic data on African freshwater fishes. We aimed to contribute to bridging this gap by providing molecular data on commercially important catfish species from the north-western part of Lake Tanganyika. We collected 215 catfish specimens and sequenced the standard vertebrate barcoding gene (COI) for 41 specimens. Additionally, we sequenced 20 specimens for the mitochondrial Cyt-b gene to make the link to previously published datasets. We identified 11 species using morphology, compared DNA sequences with those available on GenBank, and employed Automatic Barcode Gap Discovery (ABGD) and phylogenetic approaches. The dataset includes the first molecular data (COI and Cyt-b) for <i>Chrysichthys acsiorum</i>, as well as the first-ever COI sequences for <i>Dinotopterus cunningtoni</i> and <i>Malapterurus tanganyikaensis</i>. Our findings extend the known distribution of <i>C. acsiorum</i> by approximately 100 km. Additionally, we demonstrated the difficulty in delineating species of <i>Chrysichthys</i> and <i>Synodontis</i> from Lake Tanganyika with molecular tools. For <i>Chrysichthys</i>, automated methods, such as ABGD, failed to delineate species. However, barcoding does seem promising as all the individual species are resolved as clades. Within <i>Synodontis</i>, the study found a strong similarity between <i>S. grandiops</i> and <i>S. multipunctatus</i>, highlighting a need for revision. Our findings emphasize the necessity for integrative taxonomy in the study of catfishes from Lake Tanganyika.https://www.mdpi.com/1424-2818/17/1/8COICyt-btaxonomy<i>Synodontis</i><i>Chrysichthys</i><i>Chrysichthys acsiorum</i> |
| spellingShingle | Archimède Mushagalusa Mulega Arthur F. Boom Imane Rahmouni Hocein Bazairi Abdelaziz Benhoussa Maarten P. M. Vanhove Maarten Van Steenberge Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes Diversity COI Cyt-b taxonomy <i>Synodontis</i> <i>Chrysichthys</i> <i>Chrysichthys acsiorum</i> |
| title | Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes |
| title_full | Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes |
| title_fullStr | Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes |
| title_full_unstemmed | Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes |
| title_short | Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes |
| title_sort | catfishes from the north western part of lake tanganyika contribution to a reference library of dna barcodes |
| topic | COI Cyt-b taxonomy <i>Synodontis</i> <i>Chrysichthys</i> <i>Chrysichthys acsiorum</i> |
| url | https://www.mdpi.com/1424-2818/17/1/8 |
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