Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish
Abstract Background Previous studies found that Polygonatum sibiricum polysaccharide (PSP) exhibited anti-inflammatory and anti-oxidative effects on type 2 diabetes (T2DM). However, the roles and detailed mechanisms of PSP on the anti-diabetic osteoporosis are still poorly defined. The purpose of th...
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BMC
2025-07-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-025-04978-9 |
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| author | Ziping Chen Na Li Zhou Zhou Lin Zhu Kai Wang Shijin Chen Quan Gu Yujun Liu Kefeng Zhai Shuwen Xu |
| author_facet | Ziping Chen Na Li Zhou Zhou Lin Zhu Kai Wang Shijin Chen Quan Gu Yujun Liu Kefeng Zhai Shuwen Xu |
| author_sort | Ziping Chen |
| collection | DOAJ |
| description | Abstract Background Previous studies found that Polygonatum sibiricum polysaccharide (PSP) exhibited anti-inflammatory and anti-oxidative effects on type 2 diabetes (T2DM). However, the roles and detailed mechanisms of PSP on the anti-diabetic osteoporosis are still poorly defined. The purpose of this study was to identify the detailed mechanisms of PSP-induced anti-diabetic osteoporosis. Methods The roles of PSP on diabetic osteoporosis were evaluated on a T2DM zebrafish model. Furthermore, the changes of related physiological indicators and metabolites in adult zebrafish (Danio rerio) were investigated by pharmacology and metabolomic analyses. Results Results showed that PSP significantly reduced the blood glucose content and induced caudal fin regeneration. Metabolomic analysis found that the key metabolites involved in arachidonic acid metabolism, PPAR signaling pathway, linoleic acid metabolism, steroid hormone biosynthesis, amino acid biosynthesis and metabolism, alpha-Linolenic acid metabolism, sphingolipid signaling pathway were affected by PSP treatment. Among these, PSP reversed the trend changes of several metabolites, such as L-aspartic acid, choline, and propionylcarnitine. Besides, further changes of TNF-α and IL-1β transcripts suggested that the proinflammatory cytokines were also involved in the PSP-triggered anti-diabetic osteoporosis. Conclusion Above findings provided the theoretical basis for the use to plant PSP as food ingredients or bioactive compounds in natural health products on diabetic osteoporosis. |
| format | Article |
| id | doaj-art-e0fccf41a2ef4ed08fd03c8bf5a5180a |
| institution | Kabale University |
| issn | 2662-7671 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj-art-e0fccf41a2ef4ed08fd03c8bf5a5180a2025-08-20T03:45:45ZengBMCBMC Complementary Medicine and Therapies2662-76712025-07-0125111510.1186/s12906-025-04978-9Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafishZiping Chen0Na Li1Zhou Zhou2Lin Zhu3Kai Wang4Shijin Chen5Quan Gu6Yujun Liu7Kefeng Zhai8Shuwen Xu9Anhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologyAnhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologyAnhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologyCollege of Life Sciences, Nanjing Normal UniversityAnhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologyAnhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologyAnhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologyAnhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologySchool of Biological and Food Engineering, Suzhou UniversityAnhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and TechnologyAbstract Background Previous studies found that Polygonatum sibiricum polysaccharide (PSP) exhibited anti-inflammatory and anti-oxidative effects on type 2 diabetes (T2DM). However, the roles and detailed mechanisms of PSP on the anti-diabetic osteoporosis are still poorly defined. The purpose of this study was to identify the detailed mechanisms of PSP-induced anti-diabetic osteoporosis. Methods The roles of PSP on diabetic osteoporosis were evaluated on a T2DM zebrafish model. Furthermore, the changes of related physiological indicators and metabolites in adult zebrafish (Danio rerio) were investigated by pharmacology and metabolomic analyses. Results Results showed that PSP significantly reduced the blood glucose content and induced caudal fin regeneration. Metabolomic analysis found that the key metabolites involved in arachidonic acid metabolism, PPAR signaling pathway, linoleic acid metabolism, steroid hormone biosynthesis, amino acid biosynthesis and metabolism, alpha-Linolenic acid metabolism, sphingolipid signaling pathway were affected by PSP treatment. Among these, PSP reversed the trend changes of several metabolites, such as L-aspartic acid, choline, and propionylcarnitine. Besides, further changes of TNF-α and IL-1β transcripts suggested that the proinflammatory cytokines were also involved in the PSP-triggered anti-diabetic osteoporosis. Conclusion Above findings provided the theoretical basis for the use to plant PSP as food ingredients or bioactive compounds in natural health products on diabetic osteoporosis.https://doi.org/10.1186/s12906-025-04978-9Anti-diabeticMetabolomicsOsteoporosisPolysaccharideZebrafish |
| spellingShingle | Ziping Chen Na Li Zhou Zhou Lin Zhu Kai Wang Shijin Chen Quan Gu Yujun Liu Kefeng Zhai Shuwen Xu Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish BMC Complementary Medicine and Therapies Anti-diabetic Metabolomics Osteoporosis Polysaccharide Zebrafish |
| title | Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish |
| title_full | Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish |
| title_fullStr | Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish |
| title_full_unstemmed | Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish |
| title_short | Pharmacology and metabolomic reveal Polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish |
| title_sort | pharmacology and metabolomic reveal polysaccharides from polygonatum sibiricum ameliorate the diabetic osteoporosis in zebrafish |
| topic | Anti-diabetic Metabolomics Osteoporosis Polysaccharide Zebrafish |
| url | https://doi.org/10.1186/s12906-025-04978-9 |
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