Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratio

Abstract Bioethanol production from cereal straw is a sustainable alternative to meet the current energy demand, but its low nitrogen (N) content and high carbon to nitrogen (C/N) ratio are problematic during bioethanol processing. Intercropping cereals with legumes may increase the N content and re...

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Main Authors: Md Raseduzzaman, Gokul Gaudel, Stephen Okoth Aluoch, Arbindra Timilsina
Format: Article
Language:English
Published: Springer 2025-01-01
Series:Discover Agriculture
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Online Access:https://doi.org/10.1007/s44279-025-00163-1
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author Md Raseduzzaman
Gokul Gaudel
Stephen Okoth Aluoch
Arbindra Timilsina
author_facet Md Raseduzzaman
Gokul Gaudel
Stephen Okoth Aluoch
Arbindra Timilsina
author_sort Md Raseduzzaman
collection DOAJ
description Abstract Bioethanol production from cereal straw is a sustainable alternative to meet the current energy demand, but its low nitrogen (N) content and high carbon to nitrogen (C/N) ratio are problematic during bioethanol processing. Intercropping cereals with legumes may increase the N content and reduce the C/N ratio in the harvested straw biomass. In order to investigate the combined production of food, feed, and suitable feedstock (low C/N ratio) for lignocellulosic bioethanol production, a field experiment on cereal-legume intercropping was carried out in southern Sweden. In the experiment, autumn-sown cereals (wheat, triticale, and rye) were grown as monocrops with two different N fertilization levels (60 and 120 kg N ha−1) or intercropped with autumn-sown or spring-sown (undersown) clover-grass, autumn-sown or spring-sown (undersown) alfalfa, or autumn-sown faba bean. Total grain dry matter yield, cereal grain protein content, straw dry matter yield, and straw biomass C/N ratio were estimated. The results showed that the intercrops produced more straw biomass and had a lower C/N ratio, but produced less grain, than cereal monocrops. Among the cereals, rye showed significantly higher grain and straw yields, but lower grain protein content, in all treatments. Among the intercrop treatments, cereal with spring-sown clover-grass produced higher grain and straw yields and had a significantly lower C/N ratio (38:1) than the other treatments. Thus, cereal with spring-sown (undersown) clover-grass is a promising cropping system for lignocellulosic bioethanol production.
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institution Kabale University
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spelling doaj-art-e0a3a2ee5b3846aeaf5d0b02a53c422e2025-01-19T12:36:14ZengSpringerDiscover Agriculture2731-95982025-01-013111310.1007/s44279-025-00163-1Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratioMd Raseduzzaman0Gokul Gaudel1Stephen Okoth Aluoch2Arbindra Timilsina3Department of Biosystems and Technology, Swedish University of Agricultural SciencesKey Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesKey Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesKey Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesAbstract Bioethanol production from cereal straw is a sustainable alternative to meet the current energy demand, but its low nitrogen (N) content and high carbon to nitrogen (C/N) ratio are problematic during bioethanol processing. Intercropping cereals with legumes may increase the N content and reduce the C/N ratio in the harvested straw biomass. In order to investigate the combined production of food, feed, and suitable feedstock (low C/N ratio) for lignocellulosic bioethanol production, a field experiment on cereal-legume intercropping was carried out in southern Sweden. In the experiment, autumn-sown cereals (wheat, triticale, and rye) were grown as monocrops with two different N fertilization levels (60 and 120 kg N ha−1) or intercropped with autumn-sown or spring-sown (undersown) clover-grass, autumn-sown or spring-sown (undersown) alfalfa, or autumn-sown faba bean. Total grain dry matter yield, cereal grain protein content, straw dry matter yield, and straw biomass C/N ratio were estimated. The results showed that the intercrops produced more straw biomass and had a lower C/N ratio, but produced less grain, than cereal monocrops. Among the cereals, rye showed significantly higher grain and straw yields, but lower grain protein content, in all treatments. Among the intercrop treatments, cereal with spring-sown clover-grass produced higher grain and straw yields and had a significantly lower C/N ratio (38:1) than the other treatments. Thus, cereal with spring-sown (undersown) clover-grass is a promising cropping system for lignocellulosic bioethanol production.https://doi.org/10.1007/s44279-025-00163-1Second-generation bioethanolLignocellulosic biomassC/N ratioClover-grassUndersown legumeCereal-legume intercropping
spellingShingle Md Raseduzzaman
Gokul Gaudel
Stephen Okoth Aluoch
Arbindra Timilsina
Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratio
Discover Agriculture
Second-generation bioethanol
Lignocellulosic biomass
C/N ratio
Clover-grass
Undersown legume
Cereal-legume intercropping
title Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratio
title_full Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratio
title_fullStr Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratio
title_full_unstemmed Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratio
title_short Cereal-legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon: nitrogen ratio
title_sort cereal legume intercropping enhances the quality of feedstock for lignocellulosic bioethanol production by reducing the carbon nitrogen ratio
topic Second-generation bioethanol
Lignocellulosic biomass
C/N ratio
Clover-grass
Undersown legume
Cereal-legume intercropping
url https://doi.org/10.1007/s44279-025-00163-1
work_keys_str_mv AT mdraseduzzaman cereallegumeintercroppingenhancesthequalityoffeedstockforlignocellulosicbioethanolproductionbyreducingthecarbonnitrogenratio
AT gokulgaudel cereallegumeintercroppingenhancesthequalityoffeedstockforlignocellulosicbioethanolproductionbyreducingthecarbonnitrogenratio
AT stephenokothaluoch cereallegumeintercroppingenhancesthequalityoffeedstockforlignocellulosicbioethanolproductionbyreducingthecarbonnitrogenratio
AT arbindratimilsina cereallegumeintercroppingenhancesthequalityoffeedstockforlignocellulosicbioethanolproductionbyreducingthecarbonnitrogenratio