Innovations in intestinal organoid technology featuring an open apical surface
Since the development of the three-dimensional (3D) “mini-gut” culture system, adult stem cell-derived organoid technology has rapidly advanced, providing in vitro models that replicate key cellular, molecular, and physiological properties of multiple organs. The 3D intestinal organoid system has re...
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Elsevier
2025-06-01
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| Series: | European Journal of Cell Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933525000019 |
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| author | Ye Chen Yi Wang |
| author_facet | Ye Chen Yi Wang |
| author_sort | Ye Chen |
| collection | DOAJ |
| description | Since the development of the three-dimensional (3D) “mini-gut” culture system, adult stem cell-derived organoid technology has rapidly advanced, providing in vitro models that replicate key cellular, molecular, and physiological properties of multiple organs. The 3D intestinal organoid system has resolved many long-standing challenges associated with immortalized or cancer cell cultures, offering unparalleled capabilities for modeling gastrointestinal development and diseases. However, significant limitations remain, including restricted accessibility to the epithelial apical surface for studying host-microbe interactions, interruptions in modeling chronic gastrointestinal diseases due to frequent passaging and dissociation, and the absence of mechanical cues such as peristalsis and luminal flow, which are critical for organ development and function. To address these challenges, recent advancements have introduced Transwell-based monolayer cultures and microfluidic device-based technologies including “organ-on-a-chip” and scaffold-guided 'mini-gut' system. This review highlights these innovations, with a focus on adult stem cell-derived intestinal organoid models that feature an open apical surface and discusses their prospects and challenges for advancing basic research and clinical applications. |
| format | Article |
| id | doaj-art-e09bf6e8239a4e0497c20af710cc3472 |
| institution | Kabale University |
| issn | 0171-9335 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj-art-e09bf6e8239a4e0497c20af710cc34722025-01-22T05:40:57ZengElsevierEuropean Journal of Cell Biology0171-93352025-06-011042151476Innovations in intestinal organoid technology featuring an open apical surfaceYe Chen0Yi Wang1The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, Saint Louis, MO, USA; Center for Gut Microbiome and Nutrition Research, Washington University School of Medicine, Saint Louis, MO, USAThe Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, Saint Louis, MO, USA; Center for Gut Microbiome and Nutrition Research, Washington University School of Medicine, Saint Louis, MO, USA; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA; Corresponding author at: The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, Saint Louis, MO, USA.Since the development of the three-dimensional (3D) “mini-gut” culture system, adult stem cell-derived organoid technology has rapidly advanced, providing in vitro models that replicate key cellular, molecular, and physiological properties of multiple organs. The 3D intestinal organoid system has resolved many long-standing challenges associated with immortalized or cancer cell cultures, offering unparalleled capabilities for modeling gastrointestinal development and diseases. However, significant limitations remain, including restricted accessibility to the epithelial apical surface for studying host-microbe interactions, interruptions in modeling chronic gastrointestinal diseases due to frequent passaging and dissociation, and the absence of mechanical cues such as peristalsis and luminal flow, which are critical for organ development and function. To address these challenges, recent advancements have introduced Transwell-based monolayer cultures and microfluidic device-based technologies including “organ-on-a-chip” and scaffold-guided 'mini-gut' system. This review highlights these innovations, with a focus on adult stem cell-derived intestinal organoid models that feature an open apical surface and discusses their prospects and challenges for advancing basic research and clinical applications.http://www.sciencedirect.com/science/article/pii/S01719335250000193D organoidsTranswell cultureOrgan-on-a-chipAdult tissue stem cellsPluripotent stem cellsIntestinal stem cells |
| spellingShingle | Ye Chen Yi Wang Innovations in intestinal organoid technology featuring an open apical surface European Journal of Cell Biology 3D organoids Transwell culture Organ-on-a-chip Adult tissue stem cells Pluripotent stem cells Intestinal stem cells |
| title | Innovations in intestinal organoid technology featuring an open apical surface |
| title_full | Innovations in intestinal organoid technology featuring an open apical surface |
| title_fullStr | Innovations in intestinal organoid technology featuring an open apical surface |
| title_full_unstemmed | Innovations in intestinal organoid technology featuring an open apical surface |
| title_short | Innovations in intestinal organoid technology featuring an open apical surface |
| title_sort | innovations in intestinal organoid technology featuring an open apical surface |
| topic | 3D organoids Transwell culture Organ-on-a-chip Adult tissue stem cells Pluripotent stem cells Intestinal stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S0171933525000019 |
| work_keys_str_mv | AT yechen innovationsinintestinalorganoidtechnologyfeaturinganopenapicalsurface AT yiwang innovationsinintestinalorganoidtechnologyfeaturinganopenapicalsurface |