A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach
Introduction: The current vaccine strategies to prevent cervical cancer are effective only for individuals unexposed to HPV, lacking therapeutic effects against pre-existing infections. Multiepitope vaccines, using an immunoinformatic approach, are promising against tumors and viral infections becau...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2024-09-01
|
| Series: | BioImpacts |
| Subjects: | |
| Online Access: | https://bi.tbzmed.ac.ir/PDF/bi-14-27846.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832595650900918272 |
|---|---|
| author | Sari Eka Pratiwi Ysrafil Ysrafil Mardhia Mardhia Mahyarudin Mahyarudin Muhammad Inam Ilmiawan Heru Fajar Trianto Delima Fajar Liana Yuri Amia |
| author_facet | Sari Eka Pratiwi Ysrafil Ysrafil Mardhia Mardhia Mahyarudin Mahyarudin Muhammad Inam Ilmiawan Heru Fajar Trianto Delima Fajar Liana Yuri Amia |
| author_sort | Sari Eka Pratiwi |
| collection | DOAJ |
| description | Introduction: The current vaccine strategies to prevent cervical cancer are effective only for individuals unexposed to HPV, lacking therapeutic effects against pre-existing infections. Multiepitope vaccines, using an immunoinformatic approach, are promising against tumors and viral infections because of their high specificity, safety, and stability, as well as the cheap cost of development. Methods: This study employed computer-based immunoinformatic analysis to design therapeutic multiepitope vaccines against cervical cancer using oncoproteins E6 and E7 of HPV 16 and 18. Several immunoinformatic tools were applied to analyze potential vaccine constructs capable of stimulating immune responses against both oncoproteins. Results: The constructed vaccine exhibited antigenic, immunogenic, nonallergenic, nontoxic, stable, and soluble characteristics. Additionally, it effectively interacted with TLR2 and TLR4, showing high binding capacity. Computational analysis indicated the vaccine could induce immune responses through the elevation of cytokine levels after the third injection, antibody production, activation of memory B and T cells, and promotion of increased dendritic cell counts. Conclusion: The novel multiepitope vaccine based on E6 and E7 presented as a promising candidate for combating HPV infections and associated cervical cancer. Further in vitro and in vivo studies were essential to validate the efficacy and safety of the vaccine. |
| format | Article |
| id | doaj-art-e05f080a0b7a47d69a85b138d565244e |
| institution | Kabale University |
| issn | 2228-5652 2228-5660 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj-art-e05f080a0b7a47d69a85b138d565244e2025-01-18T10:02:58ZengTabriz University of Medical SciencesBioImpacts2228-56522228-56602024-09-01145278462784610.34172/bi.2024.27846bi-27846A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approachSari Eka Pratiwi0Ysrafil Ysrafil1Mardhia Mardhia2Mahyarudin Mahyarudin3Muhammad Inam Ilmiawan4Heru Fajar Trianto5Delima Fajar Liana6Yuri Amia7Department of Biology and Pathobiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, IndonesiaDepartment of Pharmacology, Faculty of Medicine, Universitas Palangka Raya, Palangka Raya, IndonesiaDepartment of Microbiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, IndonesiaDepartment of Microbiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, IndonesiaDepartment of Biology and Pathobiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, IndonesiaDepartment of Biology and Pathobiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, IndonesiaDepartment of Microbiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, IndonesiaMedical School, Faculty of Medicine, Universitas Tanjungpura, Pontianak, IndonesiaIntroduction: The current vaccine strategies to prevent cervical cancer are effective only for individuals unexposed to HPV, lacking therapeutic effects against pre-existing infections. Multiepitope vaccines, using an immunoinformatic approach, are promising against tumors and viral infections because of their high specificity, safety, and stability, as well as the cheap cost of development. Methods: This study employed computer-based immunoinformatic analysis to design therapeutic multiepitope vaccines against cervical cancer using oncoproteins E6 and E7 of HPV 16 and 18. Several immunoinformatic tools were applied to analyze potential vaccine constructs capable of stimulating immune responses against both oncoproteins. Results: The constructed vaccine exhibited antigenic, immunogenic, nonallergenic, nontoxic, stable, and soluble characteristics. Additionally, it effectively interacted with TLR2 and TLR4, showing high binding capacity. Computational analysis indicated the vaccine could induce immune responses through the elevation of cytokine levels after the third injection, antibody production, activation of memory B and T cells, and promotion of increased dendritic cell counts. Conclusion: The novel multiepitope vaccine based on E6 and E7 presented as a promising candidate for combating HPV infections and associated cervical cancer. Further in vitro and in vivo studies were essential to validate the efficacy and safety of the vaccine.https://bi.tbzmed.ac.ir/PDF/bi-14-27846.pdfmultiepitope vaccinehpv vaccinetherapeutic vaccineimmunoinformaticreverse vaccinology |
| spellingShingle | Sari Eka Pratiwi Ysrafil Ysrafil Mardhia Mardhia Mahyarudin Mahyarudin Muhammad Inam Ilmiawan Heru Fajar Trianto Delima Fajar Liana Yuri Amia A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach BioImpacts multiepitope vaccine hpv vaccine therapeutic vaccine immunoinformatic reverse vaccinology |
| title | A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach |
| title_full | A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach |
| title_fullStr | A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach |
| title_full_unstemmed | A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach |
| title_short | A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach |
| title_sort | novel therapeutic multiepitope vaccine based on oncoprotein e6 and e7 of hpv 16 and 18 an in silico approach |
| topic | multiepitope vaccine hpv vaccine therapeutic vaccine immunoinformatic reverse vaccinology |
| url | https://bi.tbzmed.ac.ir/PDF/bi-14-27846.pdf |
| work_keys_str_mv | AT sariekapratiwi anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT ysrafilysrafil anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT mardhiamardhia anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT mahyarudinmahyarudin anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT muhammadinamilmiawan anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT herufajartrianto anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT delimafajarliana anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT yuriamia anoveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT sariekapratiwi noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT ysrafilysrafil noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT mardhiamardhia noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT mahyarudinmahyarudin noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT muhammadinamilmiawan noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT herufajartrianto noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT delimafajarliana noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach AT yuriamia noveltherapeuticmultiepitopevaccinebasedononcoproteine6ande7ofhpv16and18aninsilicoapproach |