Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli
This study aims to investigate the effects of dietary chitosan (COS) on gastrointestinal pathogen resistance in mice model. For two weeks, a control group of ICR mice received a basal diet whilst the intervention group received the basal diet supplemented with 300 mg/kg COS. After two weeks, the mic...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/7432845 |
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| author | Gang Liu Shuai Chen Guiping Guan Jun Tan Naif A. Al-Dhabi Hongbing Wang Veeramuthu Duraipandiyan Jun Fang |
| author_facet | Gang Liu Shuai Chen Guiping Guan Jun Tan Naif A. Al-Dhabi Hongbing Wang Veeramuthu Duraipandiyan Jun Fang |
| author_sort | Gang Liu |
| collection | DOAJ |
| description | This study aims to investigate the effects of dietary chitosan (COS) on gastrointestinal pathogen resistance in mice model. For two weeks, a control group of ICR mice received a basal diet whilst the intervention group received the basal diet supplemented with 300 mg/kg COS. After two weeks, the mice fed the supplemented diet had a lower body weight. Then enterotoxigenic Escherichia coli (E. coli) was administered to the mice through oral gavage, with each mouse receiving 108 CFU. At day 7 after infection, the bacterial load in the jejunum and faeces was significantly lower in the COS group than that in the control group. Moreover, the mRNA and protein levels of IL-1β, IL-6, IL-17, IL-18, and TNF-α were significantly lower in the group of mice receiving the COS diet; also the jejunal production of toll-like receptor-4 (TLR-4) was suppressed in the COS group. These results indicate the intervention influenced inflammation and controlled E. coli infection. |
| format | Article |
| id | doaj-art-df8508910df240a3863cfc6d36a92ae3 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-df8508910df240a3863cfc6d36a92ae32025-02-03T05:45:27ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/74328457432845Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coliGang Liu0Shuai Chen1Guiping Guan2Jun Tan3Naif A. Al-Dhabi4Hongbing Wang5Veeramuthu Duraipandiyan6Jun Fang7College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, ChinaInstitute of Subtropical Agriculture, Chinese Academy of Sciences, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Changsha, Hunan 410125, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, ChinaDepartment of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaHunan Institute of Animal and Veterinary Science, Changsha, Hunan 410131, ChinaDepartment of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, ChinaThis study aims to investigate the effects of dietary chitosan (COS) on gastrointestinal pathogen resistance in mice model. For two weeks, a control group of ICR mice received a basal diet whilst the intervention group received the basal diet supplemented with 300 mg/kg COS. After two weeks, the mice fed the supplemented diet had a lower body weight. Then enterotoxigenic Escherichia coli (E. coli) was administered to the mice through oral gavage, with each mouse receiving 108 CFU. At day 7 after infection, the bacterial load in the jejunum and faeces was significantly lower in the COS group than that in the control group. Moreover, the mRNA and protein levels of IL-1β, IL-6, IL-17, IL-18, and TNF-α were significantly lower in the group of mice receiving the COS diet; also the jejunal production of toll-like receptor-4 (TLR-4) was suppressed in the COS group. These results indicate the intervention influenced inflammation and controlled E. coli infection.http://dx.doi.org/10.1155/2016/7432845 |
| spellingShingle | Gang Liu Shuai Chen Guiping Guan Jun Tan Naif A. Al-Dhabi Hongbing Wang Veeramuthu Duraipandiyan Jun Fang Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli Mediators of Inflammation |
| title | Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli |
| title_full | Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli |
| title_fullStr | Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli |
| title_full_unstemmed | Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli |
| title_short | Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli |
| title_sort | chitosan modulates inflammatory responses in rats infected with enterotoxigenic escherichia coli |
| url | http://dx.doi.org/10.1155/2016/7432845 |
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