Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense
Hemocyanin is a copper-containing protein with immune function against disease. In this study, a hemocyanin subunit named MnHc-1 was cloned from Macrobrachium nipponense. The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids. T...
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2016-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2016/6404817 |
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| author | Youqin Kong Liqiao Chen Zhili Ding Jianguang Qin Shengming Sun Ligai Wang Jinyun Ye |
| author_facet | Youqin Kong Liqiao Chen Zhili Ding Jianguang Qin Shengming Sun Ligai Wang Jinyun Ye |
| author_sort | Youqin Kong |
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| description | Hemocyanin is a copper-containing protein with immune function against disease. In this study, a hemocyanin subunit named MnHc-1 was cloned from Macrobrachium nipponense. The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids. The MnHc-1 mRNA was expressed in the hepatopancreas, gill, hemocytes, intestine, ovary, and stomach, with the highest level in the hepatopancreas. In the infection trial, the MnHc-1 mRNA transcripts in the hemocytes were significantly downregulated at 3 h after injection of Aeromonas hydrophila and then upregulated at 6 h and 12 h, followed by a gradual recovery from 24 to 48 h. The MnHc-1 transcriptional expression in the hepatopancreas was measured after M. nipponense were fed seven diets with 2.8, 12.2, 20.9, 29.8, 43.1, 78.9, and 157.1 mg Cu kg−1 for 8 weeks, respectively. The level of MnHc-1 mRNA was significantly higher in the prawns fed 43.1–157.1 mg Cu kg−1 diet than in that fed 2.8–29.8 mg Cu kg−1 diet. This study indicated that the MnHc-1 expression can be affected by dietary copper and the hemocyanin may potentially participate in the antibacterial defense of M. nipponense. |
| format | Article |
| id | doaj-art-df614e8f2bd942e6b3c3a134c8262165 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | International Journal of Genomics |
| spelling | doaj-art-df614e8f2bd942e6b3c3a134c82621652025-02-03T06:42:07ZengWileyInternational Journal of Genomics2314-436X2314-43782016-01-01201610.1155/2016/64048176404817Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponenseYouqin Kong0Liqiao Chen1Zhili Ding2Jianguang Qin3Shengming Sun4Ligai Wang5Jinyun Ye6School of Life Sciences, East China Normal University, Shanghai 200062, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200062, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200062, ChinaSchool of Biological Sciences, Flinders University, Adelaide, SA 5001, AustraliaSchool of Life Sciences, East China Normal University, Shanghai 200062, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200062, ChinaSchool of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000, ChinaHemocyanin is a copper-containing protein with immune function against disease. In this study, a hemocyanin subunit named MnHc-1 was cloned from Macrobrachium nipponense. The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids. The MnHc-1 mRNA was expressed in the hepatopancreas, gill, hemocytes, intestine, ovary, and stomach, with the highest level in the hepatopancreas. In the infection trial, the MnHc-1 mRNA transcripts in the hemocytes were significantly downregulated at 3 h after injection of Aeromonas hydrophila and then upregulated at 6 h and 12 h, followed by a gradual recovery from 24 to 48 h. The MnHc-1 transcriptional expression in the hepatopancreas was measured after M. nipponense were fed seven diets with 2.8, 12.2, 20.9, 29.8, 43.1, 78.9, and 157.1 mg Cu kg−1 for 8 weeks, respectively. The level of MnHc-1 mRNA was significantly higher in the prawns fed 43.1–157.1 mg Cu kg−1 diet than in that fed 2.8–29.8 mg Cu kg−1 diet. This study indicated that the MnHc-1 expression can be affected by dietary copper and the hemocyanin may potentially participate in the antibacterial defense of M. nipponense.http://dx.doi.org/10.1155/2016/6404817 |
| spellingShingle | Youqin Kong Liqiao Chen Zhili Ding Jianguang Qin Shengming Sun Ligai Wang Jinyun Ye Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense International Journal of Genomics |
| title | Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense |
| title_full | Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense |
| title_fullStr | Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense |
| title_full_unstemmed | Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense |
| title_short | Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense |
| title_sort | molecular cloning characterization and mrna expression of hemocyanin subunit in oriental river prawn macrobrachium nipponense |
| url | http://dx.doi.org/10.1155/2016/6404817 |
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