Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep
The immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immun...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/3562672 |
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| author | João Henrique Barbosa Toscano Cintia Hiromi Okino Isabella Barbosa dos Santos Luciana Aparecida Giraldelo Marei Borsch von Haehling Sérgio Novita Esteves Ana Carolina de Souza Chagas |
| author_facet | João Henrique Barbosa Toscano Cintia Hiromi Okino Isabella Barbosa dos Santos Luciana Aparecida Giraldelo Marei Borsch von Haehling Sérgio Novita Esteves Ana Carolina de Souza Chagas |
| author_sort | João Henrique Barbosa Toscano |
| collection | DOAJ |
| description | The immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immune response in lambs of the Morada Nova Brazilian breed with contrasting H. contortus resistance phenotypes. Briefly, 287 lambs were characterized through fecal egg counts (FEC) and packed cell volume (PCV) after two independent experimental parasitic challenges with 4,000 H. contortus L3. 20 extreme resistance phenotypes (10 most resistant and 10 most susceptible) were selected, subjected to a third artificial infection with 4,000 L3, and euthanized 7 days later. Tissue samples were collected from abomasal fundic and pyloric mucosa and abomasal lymph nodes. Blood samples were collected at days 0 and 7 of the third parasitic challenge. RNA was extracted from tissue and blood samples for relative quantification of innate immune-related genes by RT-qPCR. For the abomasal fundic mucosa, increased TNFα and IL1β expression levels (P<0.05) were found in the susceptible animals, while resistant animals had IL33 superiorly expressed (P<0.05). Higher levels (P<0.05) of TLR2 and CFI were found in the abomasal pyloric mucosa of resistant animals. TNFα was at higher levels (P<0.05) in the blood of susceptible lambs, at day 0 of the third artificial infection. The exacerbated proinflammatory response observed in susceptible animals, at both local and systemic levels, may be a consequence of high H. contortus parasitism. This hypothesis is corroborated by the higher blood levels of TNFα before the onset of infection, which probably remained elevated from the previous parasitic challenges. On the other hand, resistant lambs had an enhanced response mediated by TLR recognition and complement activation. Nevertheless, this is the first study to directly associate sheep parasitic resistance with IL33, an innate trigger of the Th2-polarized response. |
| format | Article |
| id | doaj-art-df571e7476a84bcf8270c86115e9c6ea |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-df571e7476a84bcf8270c86115e9c6ea2025-02-03T01:23:44ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/35626723562672Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova SheepJoão Henrique Barbosa Toscano0Cintia Hiromi Okino1Isabella Barbosa dos Santos2Luciana Aparecida Giraldelo3Marei Borsch von Haehling4Sérgio Novita Esteves5Ana Carolina de Souza Chagas6Faculdade de Ciências Agrárias e Veterinárias, UNESP, Via de Acesso Prof. Paulo Donato Castellane, 14884-900 Jaboticabal, São Paulo, BrazilEmbrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234, Fazenda Canchim, 339, 13560-970 São Carlos, SP, BrazilFaculdade de Ciências Agrárias e Veterinárias, UNESP, Via de Acesso Prof. Paulo Donato Castellane, 14884-900 Jaboticabal, São Paulo, BrazilCentro Universitário Central Paulista, Rua Miguel Petroni, no. 5111, 13563-470 São Carlos, São Paulo, BrazilFaculdade de Ciências Agrárias e Veterinárias, UNESP, Via de Acesso Prof. Paulo Donato Castellane, 14884-900 Jaboticabal, São Paulo, BrazilEmbrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234, Fazenda Canchim, 339, 13560-970 São Carlos, SP, BrazilEmbrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234, Fazenda Canchim, 339, 13560-970 São Carlos, SP, BrazilThe immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immune response in lambs of the Morada Nova Brazilian breed with contrasting H. contortus resistance phenotypes. Briefly, 287 lambs were characterized through fecal egg counts (FEC) and packed cell volume (PCV) after two independent experimental parasitic challenges with 4,000 H. contortus L3. 20 extreme resistance phenotypes (10 most resistant and 10 most susceptible) were selected, subjected to a third artificial infection with 4,000 L3, and euthanized 7 days later. Tissue samples were collected from abomasal fundic and pyloric mucosa and abomasal lymph nodes. Blood samples were collected at days 0 and 7 of the third parasitic challenge. RNA was extracted from tissue and blood samples for relative quantification of innate immune-related genes by RT-qPCR. For the abomasal fundic mucosa, increased TNFα and IL1β expression levels (P<0.05) were found in the susceptible animals, while resistant animals had IL33 superiorly expressed (P<0.05). Higher levels (P<0.05) of TLR2 and CFI were found in the abomasal pyloric mucosa of resistant animals. TNFα was at higher levels (P<0.05) in the blood of susceptible lambs, at day 0 of the third artificial infection. The exacerbated proinflammatory response observed in susceptible animals, at both local and systemic levels, may be a consequence of high H. contortus parasitism. This hypothesis is corroborated by the higher blood levels of TNFα before the onset of infection, which probably remained elevated from the previous parasitic challenges. On the other hand, resistant lambs had an enhanced response mediated by TLR recognition and complement activation. Nevertheless, this is the first study to directly associate sheep parasitic resistance with IL33, an innate trigger of the Th2-polarized response.http://dx.doi.org/10.1155/2019/3562672 |
| spellingShingle | João Henrique Barbosa Toscano Cintia Hiromi Okino Isabella Barbosa dos Santos Luciana Aparecida Giraldelo Marei Borsch von Haehling Sérgio Novita Esteves Ana Carolina de Souza Chagas Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep Journal of Immunology Research |
| title | Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep |
| title_full | Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep |
| title_fullStr | Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep |
| title_full_unstemmed | Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep |
| title_short | Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep |
| title_sort | innate immune responses associated with resistance against haemonchus contortus in morada nova sheep |
| url | http://dx.doi.org/10.1155/2019/3562672 |
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