Association of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian population
Numerous studies emphasize genetic contributions to schizophrenia, particularly focusing on genes coding for proteins in the dopaminergic pathway, which are extensively studied for their involvement in the disorder’s pathophysiology. This investigation aimed to examine the potential association betw...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2024-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400023B.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582061805797376 |
|---|---|
| author | Boukhenaf Yasmina Sariyah Ayachi Ouarda Achou Rayene Iness Bernou Amina Zohra Madoui Fatima Sifi Karima Larbi Rezgoun Mohamed |
| author_facet | Boukhenaf Yasmina Sariyah Ayachi Ouarda Achou Rayene Iness Bernou Amina Zohra Madoui Fatima Sifi Karima Larbi Rezgoun Mohamed |
| author_sort | Boukhenaf Yasmina |
| collection | DOAJ |
| description | Numerous studies emphasize genetic contributions to schizophrenia, particularly focusing on genes coding for proteins in the dopaminergic pathway, which are extensively studied for their involvement in the disorder’s pathophysiology. This investigation aimed to examine the potential association between the dopamine receptor D2 (DRD2) -141C insertion/ deletion (rs1799732) and the dopamine beta-hydroxylase (DBH) 19 bp insertion/deletion (rs72393728) polymorphisms with schizophrenia in an eastern Algerian population. A case-control study was conducted, involving 145 patients and 146 healthy controls. DNA samples were extracted from peripheral blood cells using the salting out technique. Genotyping for the DRD2 rs1799732 polymorphism was performed using the PCR-RFLP method, while the DBH rs72393728 polymorphism was analyzed using the PCR method. The results revealed a significant association between the DRD2 rs1799732 polymorphism and schizophrenia, evidenced by significant differences in genotypic and allelic distributions between patients and controls (P=0.001 and P=0.001, respectively). However, no statistical differences were found for the DBH rs72393728 polymorphism between patients and controls for genotype (P=0.46) or allele frequencies (P=0.73). This study supports an association between DRD2 rs1799732 polymorphism and schizophrenia in this population while finding no such association with DBH rs72393728 polymorphism. However, there may be a potential interaction between both polymorphisms. |
| format | Article |
| id | doaj-art-df33ed1edd7d445383c5e4e1a838128d |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj-art-df33ed1edd7d445383c5e4e1a838128d2025-01-30T06:38:33ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176331332410.2298/ABS240526023B0354-46642400023BAssociation of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian populationBoukhenaf Yasmina0https://orcid.org/0009-0003-5159-5921Sariyah Ayachi Ouarda1https://orcid.org/0000-0003-4522-3219Achou Rayene2https://orcid.org/0009-0004-0709-0130Iness Bernou Amina3https://orcid.org/0009-0005-6290-3058Zohra Madoui Fatima4https://orcid.org/0009-0007-6654-4085Sifi Karima5https://orcid.org/0009-0000-7132-5150Larbi Rezgoun Mohamed6https://orcid.org/0000-0003-1269-7039Laboratory of Molecular and Cellular Biology, Department of Animal Biology, University of Constantine, Constantine, AlgeriaPathology, Diagnosis and Therapy, Health and Biotechnology division, National Research Center of Biotechnology, Constantine, AlgeriaLaboratory of Molecular and Cellular Biology, Department of Animal Biology, University of Constantine, Constantine, AlgeriaLaboratory of Molecular and Cellular Biology, Department of Animal Biology, University of Constantine, Constantine, AlgeriaPsychiatric Hospital Mahmoud BELAMRI, Constantine, AlgeriaLaboratory of Biology and Molecular Genetics, University Hospital Center Benbadis, University of Constantine, Constantine, AlgeriaLaboratory of Molecular and Cellular Biology, Department of Animal Biology, University of Constantine , Constantine, AlgeriaNumerous studies emphasize genetic contributions to schizophrenia, particularly focusing on genes coding for proteins in the dopaminergic pathway, which are extensively studied for their involvement in the disorder’s pathophysiology. This investigation aimed to examine the potential association between the dopamine receptor D2 (DRD2) -141C insertion/ deletion (rs1799732) and the dopamine beta-hydroxylase (DBH) 19 bp insertion/deletion (rs72393728) polymorphisms with schizophrenia in an eastern Algerian population. A case-control study was conducted, involving 145 patients and 146 healthy controls. DNA samples were extracted from peripheral blood cells using the salting out technique. Genotyping for the DRD2 rs1799732 polymorphism was performed using the PCR-RFLP method, while the DBH rs72393728 polymorphism was analyzed using the PCR method. The results revealed a significant association between the DRD2 rs1799732 polymorphism and schizophrenia, evidenced by significant differences in genotypic and allelic distributions between patients and controls (P=0.001 and P=0.001, respectively). However, no statistical differences were found for the DBH rs72393728 polymorphism between patients and controls for genotype (P=0.46) or allele frequencies (P=0.73). This study supports an association between DRD2 rs1799732 polymorphism and schizophrenia in this population while finding no such association with DBH rs72393728 polymorphism. However, there may be a potential interaction between both polymorphisms.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400023B.pdfdopaminegenetic polymorphismsdrd2dbhschizophrenia |
| spellingShingle | Boukhenaf Yasmina Sariyah Ayachi Ouarda Achou Rayene Iness Bernou Amina Zohra Madoui Fatima Sifi Karima Larbi Rezgoun Mohamed Association of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian population Archives of Biological Sciences dopamine genetic polymorphisms drd2 dbh schizophrenia |
| title | Association of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian population |
| title_full | Association of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian population |
| title_fullStr | Association of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian population |
| title_full_unstemmed | Association of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian population |
| title_short | Association of dopamine receptor D2 -141C insertion/deletion and dopamine beta-hydroxylase 19 bp insertion/deletion polymorphisms with schizophrenia: A case-control study in the eastern Algerian population |
| title_sort | association of dopamine receptor d2 141c insertion deletion and dopamine beta hydroxylase 19 bp insertion deletion polymorphisms with schizophrenia a case control study in the eastern algerian population |
| topic | dopamine genetic polymorphisms drd2 dbh schizophrenia |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400023B.pdf |
| work_keys_str_mv | AT boukhenafyasmina associationofdopaminereceptord2141cinsertiondeletionanddopaminebetahydroxylase19bpinsertiondeletionpolymorphismswithschizophreniaacasecontrolstudyintheeasternalgerianpopulation AT sariyahayachiouarda associationofdopaminereceptord2141cinsertiondeletionanddopaminebetahydroxylase19bpinsertiondeletionpolymorphismswithschizophreniaacasecontrolstudyintheeasternalgerianpopulation AT achourayene associationofdopaminereceptord2141cinsertiondeletionanddopaminebetahydroxylase19bpinsertiondeletionpolymorphismswithschizophreniaacasecontrolstudyintheeasternalgerianpopulation AT inessbernouamina associationofdopaminereceptord2141cinsertiondeletionanddopaminebetahydroxylase19bpinsertiondeletionpolymorphismswithschizophreniaacasecontrolstudyintheeasternalgerianpopulation AT zohramadouifatima associationofdopaminereceptord2141cinsertiondeletionanddopaminebetahydroxylase19bpinsertiondeletionpolymorphismswithschizophreniaacasecontrolstudyintheeasternalgerianpopulation AT sifikarima associationofdopaminereceptord2141cinsertiondeletionanddopaminebetahydroxylase19bpinsertiondeletionpolymorphismswithschizophreniaacasecontrolstudyintheeasternalgerianpopulation AT larbirezgounmohamed associationofdopaminereceptord2141cinsertiondeletionanddopaminebetahydroxylase19bpinsertiondeletionpolymorphismswithschizophreniaacasecontrolstudyintheeasternalgerianpopulation |