Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated Resistance
Macromolecule bactericides present challenges such as low biocompatibility and not being biodegradable, so broad-spectrum bactericides without accumulated bacteria resistance are now in urgent demand all over the world. Lysozyme, a kind of wide-spread natural enzyme easily extracted from nature, has...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/2/232 |
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| author | Zhenhui Li Song Lin Mei Zhu Xiaoman Liu Xin Huang |
| author_facet | Zhenhui Li Song Lin Mei Zhu Xiaoman Liu Xin Huang |
| author_sort | Zhenhui Li |
| collection | DOAJ |
| description | Macromolecule bactericides present challenges such as low biocompatibility and not being biodegradable, so broad-spectrum bactericides without accumulated bacteria resistance are now in urgent demand all over the world. Lysozyme, a kind of wide-spread natural enzyme easily extracted from nature, has become attractive for agriculture and medicine use. However, Gram-negative bacterial strains are highly resistant to natural lysozymes, which limits their practical application. In this study, rather than directly modifying antibacterial-active substance with lysozyme, we show an effective way to improve antibacterial performance by altering the hydrophobic functional groups of natural lysozymes and synthesize a type of hydrophobic modified lysozyme (HML). Compared with other modification methods, the antibacterial performance has been increased by over 50%. We investigated its antibacterial mechanism against Gram-negative bacteria and showed that HML could be used to treat pathogenic bacteria without obvious accumulated resistance appearance, which is a great advantage over commercial antibiotics. Overall, it is anticipated that HML could be potentially applied to food safety, infection therapy, and enzyme-medicine applications. |
| format | Article |
| id | doaj-art-de4d60e42a4d4474a0564206a6323fde |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-de4d60e42a4d4474a0564206a6323fde2025-01-24T13:43:12ZengMDPI AGMolecules1420-30492025-01-0130223210.3390/molecules30020232Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated ResistanceZhenhui Li0Song Lin1Mei Zhu2Xiaoman Liu3Xin Huang4School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaMacromolecule bactericides present challenges such as low biocompatibility and not being biodegradable, so broad-spectrum bactericides without accumulated bacteria resistance are now in urgent demand all over the world. Lysozyme, a kind of wide-spread natural enzyme easily extracted from nature, has become attractive for agriculture and medicine use. However, Gram-negative bacterial strains are highly resistant to natural lysozymes, which limits their practical application. In this study, rather than directly modifying antibacterial-active substance with lysozyme, we show an effective way to improve antibacterial performance by altering the hydrophobic functional groups of natural lysozymes and synthesize a type of hydrophobic modified lysozyme (HML). Compared with other modification methods, the antibacterial performance has been increased by over 50%. We investigated its antibacterial mechanism against Gram-negative bacteria and showed that HML could be used to treat pathogenic bacteria without obvious accumulated resistance appearance, which is a great advantage over commercial antibiotics. Overall, it is anticipated that HML could be potentially applied to food safety, infection therapy, and enzyme-medicine applications.https://www.mdpi.com/1420-3049/30/2/232antibacteriallysozymehydrophobic modificationbiocompatibility |
| spellingShingle | Zhenhui Li Song Lin Mei Zhu Xiaoman Liu Xin Huang Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated Resistance Molecules antibacterial lysozyme hydrophobic modification biocompatibility |
| title | Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated Resistance |
| title_full | Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated Resistance |
| title_fullStr | Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated Resistance |
| title_full_unstemmed | Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated Resistance |
| title_short | Enhanced Antibacterial Activity of Hydrophobic Modified Lysozyme Against Gram-Negative Bacteria Without Accumulated Resistance |
| title_sort | enhanced antibacterial activity of hydrophobic modified lysozyme against gram negative bacteria without accumulated resistance |
| topic | antibacterial lysozyme hydrophobic modification biocompatibility |
| url | https://www.mdpi.com/1420-3049/30/2/232 |
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