Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth
Abstract Antibacterial and antiviral coating materials have attracted increasing attention for the prevention of infections caused by frequently touched surfaces in communities and hospitals. The standard assessment procedure for antibacterial surfaces involves bacterial culture on a film-covered su...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-81945-3 |
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| author | Noriko Nakamura Tatsuki Yamagishi Wataru Nagata Yuki Akahane Hyemin Seo Ayaka Otsuka Daiki Hishikawa Risa Yoshimoto Nobuhiro Miyamae Seiichi Ohta |
| author_facet | Noriko Nakamura Tatsuki Yamagishi Wataru Nagata Yuki Akahane Hyemin Seo Ayaka Otsuka Daiki Hishikawa Risa Yoshimoto Nobuhiro Miyamae Seiichi Ohta |
| author_sort | Noriko Nakamura |
| collection | DOAJ |
| description | Abstract Antibacterial and antiviral coating materials have attracted increasing attention for the prevention of infections caused by frequently touched surfaces in communities and hospitals. The standard assessment procedure for antibacterial surfaces involves bacterial culture on a film-covered substrate followed by transfer onto agar for colony counting (ISO22196:2011). However, this assessment lacks temporal and spatial information regarding bacterial growth, resulting in an incomplete and inaccurate evaluation of the antibacterial activity of the surface. In this study, we develop a novel evaluation procedure for antibacterial substrates that enables in situ visualization of bacterial growth on a surface with centimeter-scale spatial information using fluorescent protein-expressing bacterial cells and an image acquisition setup. The effects of equipment parameters on bacterial growth are systematically investigated to establish the standard evaluation conditions. Based on the optimized parameters, a quantitative evaluation of the antibacterial activity of the coating material is successfully demonstrated. The proposed method is expected to be useful in investigating the spatial and temporal distribution of bacterial growth on substrates. |
| format | Article |
| id | doaj-art-dd295cb9e1354b53b1b0ee965ccb1f85 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-dd295cb9e1354b53b1b0ee965ccb1f852025-02-02T12:19:49ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-024-81945-3Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growthNoriko Nakamura0Tatsuki Yamagishi1Wataru Nagata2Yuki Akahane3Hyemin Seo4Ayaka Otsuka5Daiki Hishikawa6Risa Yoshimoto7Nobuhiro Miyamae8Seiichi Ohta9Institute of Engineering Innovation, University of TokyoDepartment of Bioengineering, University of TokyoDepartment of Chemical System Engineering, University of TokyoDepartment of Chemical System Engineering, University of TokyoDepartment of Bioengineering, University of TokyoDepartment of Bioengineering, University of TokyoNippon Paint Co., LtdNippon Paint Co., LtdNippon Paint Co., LtdInstitute of Engineering Innovation, University of TokyoAbstract Antibacterial and antiviral coating materials have attracted increasing attention for the prevention of infections caused by frequently touched surfaces in communities and hospitals. The standard assessment procedure for antibacterial surfaces involves bacterial culture on a film-covered substrate followed by transfer onto agar for colony counting (ISO22196:2011). However, this assessment lacks temporal and spatial information regarding bacterial growth, resulting in an incomplete and inaccurate evaluation of the antibacterial activity of the surface. In this study, we develop a novel evaluation procedure for antibacterial substrates that enables in situ visualization of bacterial growth on a surface with centimeter-scale spatial information using fluorescent protein-expressing bacterial cells and an image acquisition setup. The effects of equipment parameters on bacterial growth are systematically investigated to establish the standard evaluation conditions. Based on the optimized parameters, a quantitative evaluation of the antibacterial activity of the coating material is successfully demonstrated. The proposed method is expected to be useful in investigating the spatial and temporal distribution of bacterial growth on substrates.https://doi.org/10.1038/s41598-024-81945-3Antibacterial activityBacterial growthFluorescent observationin situ visualization |
| spellingShingle | Noriko Nakamura Tatsuki Yamagishi Wataru Nagata Yuki Akahane Hyemin Seo Ayaka Otsuka Daiki Hishikawa Risa Yoshimoto Nobuhiro Miyamae Seiichi Ohta Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth Scientific Reports Antibacterial activity Bacterial growth Fluorescent observation in situ visualization |
| title | Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth |
| title_full | Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth |
| title_fullStr | Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth |
| title_full_unstemmed | Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth |
| title_short | Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth |
| title_sort | systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth |
| topic | Antibacterial activity Bacterial growth Fluorescent observation in situ visualization |
| url | https://doi.org/10.1038/s41598-024-81945-3 |
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