Plantago major leaf extract against bacteria and fungi of medical importance
Abstract Antimicrobial drug resistance is a challenge to public health. Various microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, carbapenemase-producing Klebsiella pneumoniae, Pythium insidiosum, and Candida auris, have developed resistance to com...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2025-01-01
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100318&lng=en&tlng=en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592535249223680 |
|---|---|
| author | Débora Silva Marques de Sousa Luciana da Silva Ruiz Ana Flávia Marques Pereira Alessandra Aguirra Sani Tatiane Baptista Zapata Gabrielle Pires de Morais Monari Cassiana Ferreira da Rosa Ary Fernandes Junior Vera Lucia Mores Rall Diego Godina Prado Alberto de Oliveira Sandra de Moraes Gimenes Bosco |
| author_facet | Débora Silva Marques de Sousa Luciana da Silva Ruiz Ana Flávia Marques Pereira Alessandra Aguirra Sani Tatiane Baptista Zapata Gabrielle Pires de Morais Monari Cassiana Ferreira da Rosa Ary Fernandes Junior Vera Lucia Mores Rall Diego Godina Prado Alberto de Oliveira Sandra de Moraes Gimenes Bosco |
| author_sort | Débora Silva Marques de Sousa |
| collection | DOAJ |
| description | Abstract Antimicrobial drug resistance is a challenge to public health. Various microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, carbapenemase-producing Klebsiella pneumoniae, Pythium insidiosum, and Candida auris, have developed resistance to commonly used antimicrobials in infection disease treatments. Consequently, there is an urgent need to explore and develop novel therapeutic drugs. Natural products, particularly medicinal plants, have received attention in the quest for innovative treatments for various diseases. Plantago major is a plant endowed with several biological properties, such as antibacterial, antifungal, and healing actions. This study aimed to investigate the antimicrobial activities of the methanolic extract obtained from the leaves of P. major. Microdilution assays were conducted to determine the minimum inhibitory concentration and minimum bactericidal and fungicidal concentration. Additionally, synergism with antimicrobial drugs was assessed using a time-kill curve analysis. A synergistic bactericidal interaction between the extract and imipenem was observed against carbapenemase-producing K. pneumoniae. For MRSA, a bacteriostatic synergism was noted in combinations of the extract with cephalotin and oxacillin. For C. auris, a fungistatic interaction was observed between the extract and amphotericin B. These results suggest the presence of bioactive compounds within the extract with therapeutic potential for combating infections caused by these microorganisms. |
| format | Article |
| id | doaj-art-dc72e4137d4a4846a10a006b4f2742b8 |
| institution | Kabale University |
| issn | 2175-9790 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj-art-dc72e4137d4a4846a10a006b4f2742b82025-01-21T07:41:23ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902025-01-016110.1590/s2175-97902025e24143Plantago major leaf extract against bacteria and fungi of medical importanceDébora Silva Marques de Sousahttps://orcid.org/0000-0002-7301-7909Luciana da Silva Ruizhttps://orcid.org/0000-0001-7374-8640Ana Flávia Marques Pereirahttps://orcid.org/0000-0002-2925-8545Alessandra Aguirra Sanihttps://orcid.org/0000-0002-0828-9799Tatiane Baptista Zapatahttps://orcid.org/0000-0002-7653-184XGabrielle Pires de Morais Monarihttps://orcid.org/0009-0008-5222-8417Cassiana Ferreira da RosaAry Fernandes Juniorhttps://orcid.org/0000-0002-2744-2824Vera Lucia Mores Rallhttps://orcid.org/0000-0003-4280-5619Diego Godina Pradohttps://orcid.org/0000-0002-7192-7765Alberto de Oliveirahttps://orcid.org/0000-0002-7210-164XSandra de Moraes Gimenes Boscohttps://orcid.org/0000-0002-6363-3740Abstract Antimicrobial drug resistance is a challenge to public health. Various microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, carbapenemase-producing Klebsiella pneumoniae, Pythium insidiosum, and Candida auris, have developed resistance to commonly used antimicrobials in infection disease treatments. Consequently, there is an urgent need to explore and develop novel therapeutic drugs. Natural products, particularly medicinal plants, have received attention in the quest for innovative treatments for various diseases. Plantago major is a plant endowed with several biological properties, such as antibacterial, antifungal, and healing actions. This study aimed to investigate the antimicrobial activities of the methanolic extract obtained from the leaves of P. major. Microdilution assays were conducted to determine the minimum inhibitory concentration and minimum bactericidal and fungicidal concentration. Additionally, synergism with antimicrobial drugs was assessed using a time-kill curve analysis. A synergistic bactericidal interaction between the extract and imipenem was observed against carbapenemase-producing K. pneumoniae. For MRSA, a bacteriostatic synergism was noted in combinations of the extract with cephalotin and oxacillin. For C. auris, a fungistatic interaction was observed between the extract and amphotericin B. These results suggest the presence of bioactive compounds within the extract with therapeutic potential for combating infections caused by these microorganisms.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100318&lng=en&tlng=enSynergistic interactionNatural productsMulti-drug resistant microorganismsAntimicrobial drugsExtract chemical compositionMass spectrometry |
| spellingShingle | Débora Silva Marques de Sousa Luciana da Silva Ruiz Ana Flávia Marques Pereira Alessandra Aguirra Sani Tatiane Baptista Zapata Gabrielle Pires de Morais Monari Cassiana Ferreira da Rosa Ary Fernandes Junior Vera Lucia Mores Rall Diego Godina Prado Alberto de Oliveira Sandra de Moraes Gimenes Bosco Plantago major leaf extract against bacteria and fungi of medical importance Brazilian Journal of Pharmaceutical Sciences Synergistic interaction Natural products Multi-drug resistant microorganisms Antimicrobial drugs Extract chemical composition Mass spectrometry |
| title | Plantago major leaf extract against bacteria and fungi of medical importance |
| title_full | Plantago major leaf extract against bacteria and fungi of medical importance |
| title_fullStr | Plantago major leaf extract against bacteria and fungi of medical importance |
| title_full_unstemmed | Plantago major leaf extract against bacteria and fungi of medical importance |
| title_short | Plantago major leaf extract against bacteria and fungi of medical importance |
| title_sort | plantago major leaf extract against bacteria and fungi of medical importance |
| topic | Synergistic interaction Natural products Multi-drug resistant microorganisms Antimicrobial drugs Extract chemical composition Mass spectrometry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100318&lng=en&tlng=en |
| work_keys_str_mv | AT deborasilvamarquesdesousa plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT lucianadasilvaruiz plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT anaflaviamarquespereira plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT alessandraaguirrasani plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT tatianebaptistazapata plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT gabriellepiresdemoraismonari plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT cassianaferreiradarosa plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT aryfernandesjunior plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT veraluciamoresrall plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT diegogodinaprado plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT albertodeoliveira plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance AT sandrademoraesgimenesbosco plantagomajorleafextractagainstbacteriaandfungiofmedicalimportance |