Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen Bacteria
One of the major challenges in healthcare is the rise of antibiotic resistance, where bacteria have developed resistance to a wide range of commonly available antibiotics. These resilient bacteria pose a significant threat to public health, leading to severe illnesses and creating a substantial chal...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Mazandaran
2024-02-01
|
| Series: | Journal of Genetic Resources |
| Subjects: | |
| Online Access: | https://sc.journals.umz.ac.ir/article_4755_023e54031d4b548a4b28cb5b275eb663.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585101627621376 |
|---|---|
| author | Zahra Mortazavi Mohamad Reza Islami Moj Khaleghi |
| author_facet | Zahra Mortazavi Mohamad Reza Islami Moj Khaleghi |
| author_sort | Zahra Mortazavi |
| collection | DOAJ |
| description | One of the major challenges in healthcare is the rise of antibiotic resistance, where bacteria have developed resistance to a wide range of commonly available antibiotics. These resilient bacteria pose a significant threat to public health, leading to severe illnesses and creating a substantial challenge for treatment. Therefore, the discovery of new antimicrobial agents is crucial in controlling the spread of infections caused by drug-resistant bacteria. This study focuses on the synthesis of oxazoline and investigates the antimicrobial and anti-biofilm properties of this compound named 4-benzylidene-2-methyl-oxazoline-5-one. The structure of the oxazoline compound was precisely characterized by 1H NMR, 13C NMR, and FT-IR. The antibacterial activity was assessed on S. aureus using the agar-well diffusion while the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined to identify the concentration ranges with significant inhibitory effects. S. aureus is one of the most noticeable microorganisms in medical and clinical sciences, especially nosocomial infections. Additionally, the study evaluated the compound's impact on biofilm formation and the expression of the icaA gene. The results from the MIC and MBC testing demonstrated that the compound exhibits both bacteriostatic and bactericidal effects on both Gram-positive and Gram-negative bacteria, as well as yeast. Furthermore, the presence of this antibacterial compound led to a reduction in icaA gene expression. 4-Benzylidene-2-methyl-oxazoline-5-one displayed significant antimicrobial activity and hindered biofilm formation. Moreover, it was observed that 4-benzylidene-2-methyl-oxazoline-5-one induced cell death through its toxic effects on MCF-7 cells. When it was tested at concentrations of 0.01, 0.1, and 1 mg/ml this compound exhibited cytotoxic effects and significantly decreased cell viability. |
| format | Article |
| id | doaj-art-dc2293638bf04f52ab6634a9120cba38 |
| institution | Kabale University |
| issn | 2423-4257 2588-2589 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | University of Mazandaran |
| record_format | Article |
| series | Journal of Genetic Resources |
| spelling | doaj-art-dc2293638bf04f52ab6634a9120cba382025-01-27T06:11:19ZengUniversity of MazandaranJournal of Genetic Resources2423-42572588-25892024-02-0110191910.22080/jgr.2024.26199.13774755Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen BacteriaZahra Mortazavi0Mohamad Reza Islami1Moj Khaleghi2Department of Chemical Engineering, Sirjan University of Technology, Sirjan, IranDepartment of Chemistry, Shahid Bahonar University of Kerman, 76169 Kerman, IranDepartment of Biology, Shahid Bahonar University of Kerman, 76169 Kerman, IranOne of the major challenges in healthcare is the rise of antibiotic resistance, where bacteria have developed resistance to a wide range of commonly available antibiotics. These resilient bacteria pose a significant threat to public health, leading to severe illnesses and creating a substantial challenge for treatment. Therefore, the discovery of new antimicrobial agents is crucial in controlling the spread of infections caused by drug-resistant bacteria. This study focuses on the synthesis of oxazoline and investigates the antimicrobial and anti-biofilm properties of this compound named 4-benzylidene-2-methyl-oxazoline-5-one. The structure of the oxazoline compound was precisely characterized by 1H NMR, 13C NMR, and FT-IR. The antibacterial activity was assessed on S. aureus using the agar-well diffusion while the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined to identify the concentration ranges with significant inhibitory effects. S. aureus is one of the most noticeable microorganisms in medical and clinical sciences, especially nosocomial infections. Additionally, the study evaluated the compound's impact on biofilm formation and the expression of the icaA gene. The results from the MIC and MBC testing demonstrated that the compound exhibits both bacteriostatic and bactericidal effects on both Gram-positive and Gram-negative bacteria, as well as yeast. Furthermore, the presence of this antibacterial compound led to a reduction in icaA gene expression. 4-Benzylidene-2-methyl-oxazoline-5-one displayed significant antimicrobial activity and hindered biofilm formation. Moreover, it was observed that 4-benzylidene-2-methyl-oxazoline-5-one induced cell death through its toxic effects on MCF-7 cells. When it was tested at concentrations of 0.01, 0.1, and 1 mg/ml this compound exhibited cytotoxic effects and significantly decreased cell viability.https://sc.journals.umz.ac.ir/article_4755_023e54031d4b548a4b28cb5b275eb663.pdf4-benzylidene-2-methyl-oxazoline-5-oneantimicrobialbiofilmicaa gene expression |
| spellingShingle | Zahra Mortazavi Mohamad Reza Islami Moj Khaleghi Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen Bacteria Journal of Genetic Resources 4-benzylidene-2-methyl-oxazoline-5-one antimicrobial biofilm icaa gene expression |
| title | Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen Bacteria |
| title_full | Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen Bacteria |
| title_fullStr | Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen Bacteria |
| title_full_unstemmed | Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen Bacteria |
| title_short | Antimicrobial and Antibiofilm Activity of 4-Benzylidene-2-methyl-oxazoline-5-one against Pathogen Bacteria |
| title_sort | antimicrobial and antibiofilm activity of 4 benzylidene 2 methyl oxazoline 5 one against pathogen bacteria |
| topic | 4-benzylidene-2-methyl-oxazoline-5-one antimicrobial biofilm icaa gene expression |
| url | https://sc.journals.umz.ac.ir/article_4755_023e54031d4b548a4b28cb5b275eb663.pdf |
| work_keys_str_mv | AT zahramortazavi antimicrobialandantibiofilmactivityof4benzylidene2methyloxazoline5oneagainstpathogenbacteria AT mohamadrezaislami antimicrobialandantibiofilmactivityof4benzylidene2methyloxazoline5oneagainstpathogenbacteria AT mojkhaleghi antimicrobialandantibiofilmactivityof4benzylidene2methyloxazoline5oneagainstpathogenbacteria |