Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China
ABSTRACT Carbapenem-resistant Acinetobacter spp. pose a significant challenge in clinical settings due to limited treatment options for nosocomial infections. Carbapenem-hydrolyzing class D beta-lactamases are the primary cause for carbapenem resistance, while metallo-beta-lactamases (MBLs) New Delh...
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American Society for Microbiology
2025-02-01
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01158-24 |
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| author | Qingye Xu Xinli Mu Jintao He Haiyang Liu Xiaochen Liu Yanfei Wang Xiaoting Hua Yunsong Yu |
| author_facet | Qingye Xu Xinli Mu Jintao He Haiyang Liu Xiaochen Liu Yanfei Wang Xiaoting Hua Yunsong Yu |
| author_sort | Qingye Xu |
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| description | ABSTRACT Carbapenem-resistant Acinetobacter spp. pose a significant challenge in clinical settings due to limited treatment options for nosocomial infections. Carbapenem-hydrolyzing class D beta-lactamases are the primary cause for carbapenem resistance, while metallo-beta-lactamases (MBLs) New Delhi metallo beta-lactamase (NDM) and imipenemase (IMP) also contribute. This study investigated five MBL-producing Acinetobacter spp. strains isolated from a clinic in China in 2010. The blaIMP-8-carrying A1014 was the first identified CRAB among all known STPas150 isolates worldwide. Through whole-genome sequencing and the southern blot analysis, we determined that blaIMP-8 was located on a pR4WN-type plasmid and blaNDM-1 was located on four distinct pSU1904NDM-type plasmids. The blaIMP-8 gene was identified within a class 1 integron organized as a 5’-conserved segment (intI), variable region (blaIMP-8-aac(6')-Ib), and 3’-conserved segment (qacEΔ1/sul1). All available sequences of blaIMP variants in A. baumannii from the NCBI were investigated and classified into five types of class 1 integrons. All blaNDM-1 plasmids were transferable, and the blaNDM-1 genes were in a conservative region. Additionally, multiple resistance genes, including those conferring resistance to aminoglycosides, tetracyclines, and macrolides, were detected on plasmids from these strains. All strains were resistant to meropenem and imipenem, while they were all susceptible to tigecycline and intermediate to polymyxin. A207 and A1014 were susceptible to cefiderocol, and only blaIMP-8-carrying A1014 had low MIC value (4/2 µg/mL) toward cefoperazone/sulbactam. In conclusion, we characterized the phenotypic and genotypic features of one IMP-8-producing and four NDM-1-producing plasmids recovered from Acinetobacter spp. strains isolated in 2010, contributing to the understanding of the dissemination and evolution of these enzymes.IMPORTANCEGiven the low prevalence of IMP among A. baumannii and the limited sequencing technology in earlier years, research on blaIMP in A. baumannii is scarce, and genetic information on blaNDM-1-producing Acinetobacter spp. strains isolated in earlier years is limited. This study revisited five MBL-carrying Acinetobacter spp. strains isolated in 2010, characterizing their phenotypic and genotypic features. This retrospective analysis serves as a form of “bacterial archaeology,” providing evidence of the evolutionary changes in genetic elements conferring antibiotic resistance. |
| format | Article |
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| institution | Kabale University |
| issn | 2165-0497 |
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| publishDate | 2025-02-01 |
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| spelling | doaj-art-db1b79c62b00407b96283fec0eb10ac12025-02-04T14:03:41ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.01158-24Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in ChinaQingye Xu0Xinli Mu1Jintao He2Haiyang Liu3Xiaochen Liu4Yanfei Wang5Xiaoting Hua6Yunsong Yu7Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, ChinaDepartment of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaDepartment of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaCentre of Laboratory Medicine, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, ChinaDepartment of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaDepartment of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaDepartment of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaZhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, ChinaABSTRACT Carbapenem-resistant Acinetobacter spp. pose a significant challenge in clinical settings due to limited treatment options for nosocomial infections. Carbapenem-hydrolyzing class D beta-lactamases are the primary cause for carbapenem resistance, while metallo-beta-lactamases (MBLs) New Delhi metallo beta-lactamase (NDM) and imipenemase (IMP) also contribute. This study investigated five MBL-producing Acinetobacter spp. strains isolated from a clinic in China in 2010. The blaIMP-8-carrying A1014 was the first identified CRAB among all known STPas150 isolates worldwide. Through whole-genome sequencing and the southern blot analysis, we determined that blaIMP-8 was located on a pR4WN-type plasmid and blaNDM-1 was located on four distinct pSU1904NDM-type plasmids. The blaIMP-8 gene was identified within a class 1 integron organized as a 5’-conserved segment (intI), variable region (blaIMP-8-aac(6')-Ib), and 3’-conserved segment (qacEΔ1/sul1). All available sequences of blaIMP variants in A. baumannii from the NCBI were investigated and classified into five types of class 1 integrons. All blaNDM-1 plasmids were transferable, and the blaNDM-1 genes were in a conservative region. Additionally, multiple resistance genes, including those conferring resistance to aminoglycosides, tetracyclines, and macrolides, were detected on plasmids from these strains. All strains were resistant to meropenem and imipenem, while they were all susceptible to tigecycline and intermediate to polymyxin. A207 and A1014 were susceptible to cefiderocol, and only blaIMP-8-carrying A1014 had low MIC value (4/2 µg/mL) toward cefoperazone/sulbactam. In conclusion, we characterized the phenotypic and genotypic features of one IMP-8-producing and four NDM-1-producing plasmids recovered from Acinetobacter spp. strains isolated in 2010, contributing to the understanding of the dissemination and evolution of these enzymes.IMPORTANCEGiven the low prevalence of IMP among A. baumannii and the limited sequencing technology in earlier years, research on blaIMP in A. baumannii is scarce, and genetic information on blaNDM-1-producing Acinetobacter spp. strains isolated in earlier years is limited. This study revisited five MBL-carrying Acinetobacter spp. strains isolated in 2010, characterizing their phenotypic and genotypic features. This retrospective analysis serves as a form of “bacterial archaeology,” providing evidence of the evolutionary changes in genetic elements conferring antibiotic resistance.https://journals.asm.org/doi/10.1128/spectrum.01158-24MBLsClass 1 integronST150pdif |
| spellingShingle | Qingye Xu Xinli Mu Jintao He Haiyang Liu Xiaochen Liu Yanfei Wang Xiaoting Hua Yunsong Yu Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China Microbiology Spectrum MBLs Class 1 integron ST150 pdif |
| title | Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China |
| title_full | Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China |
| title_fullStr | Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China |
| title_full_unstemmed | Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China |
| title_short | Phenotypic and genotypic characterization of clinical carbapenem-resistant Acinetobacter species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China |
| title_sort | phenotypic and genotypic characterization of clinical carbapenem resistant acinetobacter species harboring the metallo beta lactamases imp 8 or ndm 1 in china |
| topic | MBLs Class 1 integron ST150 pdif |
| url | https://journals.asm.org/doi/10.1128/spectrum.01158-24 |
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