Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer
The aim of this study was to reveal the associations of microRNA miR-15a and miR-16 dysregulation with clinicopathological characteristics and prognosis in patients with colorectal cancer. As a result, we found that miR-15a and miR-16 expression, detected by quantitative real time-PCR, were both sig...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2014/364549 |
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| author | Guangfa Xiao Huihuan Tang Wei Wei Jian Li Liandong Ji Jie Ge |
| author_facet | Guangfa Xiao Huihuan Tang Wei Wei Jian Li Liandong Ji Jie Ge |
| author_sort | Guangfa Xiao |
| collection | DOAJ |
| description | The aim of this study was to reveal the associations of microRNA miR-15a and miR-16 dysregulation with clinicopathological characteristics and prognosis in patients with colorectal cancer. As a result, we found that miR-15a and miR-16 expression, detected by quantitative real time-PCR, were both significantly downregulated in colorectal cancer tissues compared with adjacent colorectal mucosa (both P<0.001). Particularly, the expression levels of miR-15a in colorectal cancer tissues were positively correlated with those of miR-16 significantly (Spearman correlation coefficient r=0.652, P<0.001). In addition, miR-15a and/or miR-16 downregulation were all significantly associated with advanced TNM stage (all P<0.05), poorly histological grade (all P<0.05), and positive lymph node metastasis (all P<0.05). Moreover, the survival analysis identified miR-15a expression, miR-16 expression, and miR-15a/miR-16 combination as independent predictors of both unfavorable overall survival and disease-free survival. Interestingly, the prognostic value of miR-15a/miR-16 combination was more significant than miR-15a or miR-16 expression alone. Collectively, the aberrant expression of miR-15a and miR-16 could be used to stratify patients with aggressive tumor progression of colorectal cancer. The combined pattern of miR-15a and miR-16 downregulation has a significant value for distinguishing patients with a worse prognosis of colorectal cancer after surgery. |
| format | Article |
| id | doaj-art-db14752faee4451da789e1b6c6a54751 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-db14752faee4451da789e1b6c6a547512025-02-03T01:10:34ZengWileyGastroenterology Research and Practice1687-61211687-630X2014-01-01201410.1155/2014/364549364549Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal CancerGuangfa Xiao0Huihuan Tang1Wei Wei2Jian Li3Liandong Ji4Jie Ge5Department of General Surgery, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, Hunan 410008, ChinaDepartment of General Surgery, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, Hunan 410008, ChinaDepartment of General Surgery, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, Hunan 410008, ChinaDepartment of General Surgery, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, Hunan 410008, ChinaDepartment of General Surgery, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, Hunan 410008, ChinaDepartment of General Surgery, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, Hunan 410008, ChinaThe aim of this study was to reveal the associations of microRNA miR-15a and miR-16 dysregulation with clinicopathological characteristics and prognosis in patients with colorectal cancer. As a result, we found that miR-15a and miR-16 expression, detected by quantitative real time-PCR, were both significantly downregulated in colorectal cancer tissues compared with adjacent colorectal mucosa (both P<0.001). Particularly, the expression levels of miR-15a in colorectal cancer tissues were positively correlated with those of miR-16 significantly (Spearman correlation coefficient r=0.652, P<0.001). In addition, miR-15a and/or miR-16 downregulation were all significantly associated with advanced TNM stage (all P<0.05), poorly histological grade (all P<0.05), and positive lymph node metastasis (all P<0.05). Moreover, the survival analysis identified miR-15a expression, miR-16 expression, and miR-15a/miR-16 combination as independent predictors of both unfavorable overall survival and disease-free survival. Interestingly, the prognostic value of miR-15a/miR-16 combination was more significant than miR-15a or miR-16 expression alone. Collectively, the aberrant expression of miR-15a and miR-16 could be used to stratify patients with aggressive tumor progression of colorectal cancer. The combined pattern of miR-15a and miR-16 downregulation has a significant value for distinguishing patients with a worse prognosis of colorectal cancer after surgery.http://dx.doi.org/10.1155/2014/364549 |
| spellingShingle | Guangfa Xiao Huihuan Tang Wei Wei Jian Li Liandong Ji Jie Ge Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer Gastroenterology Research and Practice |
| title | Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer |
| title_full | Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer |
| title_fullStr | Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer |
| title_full_unstemmed | Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer |
| title_short | Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer |
| title_sort | aberrant expression of microrna 15a and microrna 16 synergistically associates with tumor progression and prognosis in patients with colorectal cancer |
| url | http://dx.doi.org/10.1155/2014/364549 |
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