Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during Spermatogenesis
The objective of this study was to assess the toxicological effect of exposure to benzo(a)pyrene, B[a]P, on germ cells during spermatogenesis. Mice were exposed to B[a]P at 1, 10, 50, and 100 mg/kg/day for 30 days via oral ingestion. Germ cells, including spermatogonia, spermatocytes, pachytene sper...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2013/387850 |
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| author | Hueiwang Anna Jeng Silvina M. Bocca |
| author_facet | Hueiwang Anna Jeng Silvina M. Bocca |
| author_sort | Hueiwang Anna Jeng |
| collection | DOAJ |
| description | The objective of this study was to assess the toxicological effect of exposure to benzo(a)pyrene, B[a]P, on germ cells during spermatogenesis. Mice were exposed to B[a]P at 1, 10, 50, and 100 mg/kg/day for 30 days via oral ingestion. Germ cells, including spermatogonia, spermatocytes, pachytene spermatocytes, and round spermatids, were recovered from testes of mice exposed to B[a]P, while mature spermatozoa were isolated from vas deferens. Reproductive organs were collected and weighed. Apoptotic response of germ cells and mature spermatozoa were qualified using the terminal deoxynucleotidyl transferase mediated deoxy-UTP nick end labeling (TUNEL) assay. B[a]P exposure at ≤10 mg/kg/day for 30 days did not significantly alter concentrations of germ cells and mature spermatozoa and apoptotic response in germ cells and mature spermatozoa. Exposure to B[a]P at 50 and 100 mg/kg/day induced testicular atrophy and yielded a significant reduction in the concentrations of spermatogonia, spermatocytes, pachytene spermatocytes, and round spermatid cells as compared with the control. Also, mature spermatozoa experienced decreased concentrations and viability. B[a]P-exposed mice experienced a significant increase in apoptotic germ cells as compared to the control mice. However, the mice dose concentrations were not relevant for comparison to human exposure. |
| format | Article |
| id | doaj-art-db0d81db5fda4d2b9bc7444c35dbfc09 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-db0d81db5fda4d2b9bc7444c35dbfc092025-02-03T01:25:05ZengWileyJournal of Toxicology1687-81911687-82052013-01-01201310.1155/2013/387850387850Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during SpermatogenesisHueiwang Anna Jeng0Silvina M. Bocca1School of Community and Environmental Health, College of Health Sciences, Old Dominion University, 4608 Hampton Boulevard, Health Sciences Building, Room 3140, Norfolk, VA 23829, USAThe Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, VA 23829, USAThe objective of this study was to assess the toxicological effect of exposure to benzo(a)pyrene, B[a]P, on germ cells during spermatogenesis. Mice were exposed to B[a]P at 1, 10, 50, and 100 mg/kg/day for 30 days via oral ingestion. Germ cells, including spermatogonia, spermatocytes, pachytene spermatocytes, and round spermatids, were recovered from testes of mice exposed to B[a]P, while mature spermatozoa were isolated from vas deferens. Reproductive organs were collected and weighed. Apoptotic response of germ cells and mature spermatozoa were qualified using the terminal deoxynucleotidyl transferase mediated deoxy-UTP nick end labeling (TUNEL) assay. B[a]P exposure at ≤10 mg/kg/day for 30 days did not significantly alter concentrations of germ cells and mature spermatozoa and apoptotic response in germ cells and mature spermatozoa. Exposure to B[a]P at 50 and 100 mg/kg/day induced testicular atrophy and yielded a significant reduction in the concentrations of spermatogonia, spermatocytes, pachytene spermatocytes, and round spermatid cells as compared with the control. Also, mature spermatozoa experienced decreased concentrations and viability. B[a]P-exposed mice experienced a significant increase in apoptotic germ cells as compared to the control mice. However, the mice dose concentrations were not relevant for comparison to human exposure.http://dx.doi.org/10.1155/2013/387850 |
| spellingShingle | Hueiwang Anna Jeng Silvina M. Bocca Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during Spermatogenesis Journal of Toxicology |
| title | Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during Spermatogenesis |
| title_full | Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during Spermatogenesis |
| title_fullStr | Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during Spermatogenesis |
| title_full_unstemmed | Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during Spermatogenesis |
| title_short | Influence of Exposure to Benzo[a]pyrene on Mice Testicular Germ Cells during Spermatogenesis |
| title_sort | influence of exposure to benzo a pyrene on mice testicular germ cells during spermatogenesis |
| url | http://dx.doi.org/10.1155/2013/387850 |
| work_keys_str_mv | AT hueiwangannajeng influenceofexposuretobenzoapyreneonmicetesticulargermcellsduringspermatogenesis AT silvinambocca influenceofexposuretobenzoapyreneonmicetesticulargermcellsduringspermatogenesis |