Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells

Recurrence of breast cancer after radiotherapy may be partly explained by the presence of radioresistant cells. Thus, it would be desirable to develop an effective therapy against radioresistant cells. In this study, we demonstrated the intense antitumor activity of cytokine-induced killer cells aga...

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Main Authors: Qingming Guo, Danni Zhu, Xiaocui Bu, Xiaofang Wei, Changyou Li, Daiqing Gao, Xiaoqiang Wei, Xuezhen Ma, Peng Zhao
Format: Article
Language:English
Published: SAGE Publishing 2017-03-01
Series:Tumor Biology
Online Access:https://doi.org/10.1177/1010428317695961
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author Qingming Guo
Danni Zhu
Xiaocui Bu
Xiaofang Wei
Changyou Li
Daiqing Gao
Xiaoqiang Wei
Xuezhen Ma
Peng Zhao
author_facet Qingming Guo
Danni Zhu
Xiaocui Bu
Xiaofang Wei
Changyou Li
Daiqing Gao
Xiaoqiang Wei
Xuezhen Ma
Peng Zhao
author_sort Qingming Guo
collection DOAJ
description Recurrence of breast cancer after radiotherapy may be partly explained by the presence of radioresistant cells. Thus, it would be desirable to develop an effective therapy against radioresistant cells. In this study, we demonstrated the intense antitumor activity of cytokine-induced killer cells against MCF-7 and radioresistant MCF-7 cells, as revealed by cytokine-induced killer–mediated cytotoxicity, tumor cell proliferation, and tumor invasion. Radioresistant MCF-7 cells were more susceptible to cytokine-induced killer cell killing. The stronger cytotoxicity of cytokine-induced killer cells against radioresistant MCF-7 cells was dependent on the expression of major histocompatibility complex class I polypeptide–related sequence A/B on radioresistant MCF-7 cells after exposure of cytokine-induced killer cells to sensitized targets. In addition, we demonstrated that cytokine-induced killer cell treatment sensitized breast cancer cells to chemotherapy via the downregulation of TK1, TYMS, and MDR1. These results indicate that cytokine-induced killer cell treatment in combination with radiotherapy and/or chemotherapy may induce synergistic antitumor activities and represent a novel strategy for breast cancer.
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institution Kabale University
issn 1423-0380
language English
publishDate 2017-03-01
publisher SAGE Publishing
record_format Article
series Tumor Biology
spelling doaj-art-db0d3c5e57c14cf09d0c8dddfc994cb22025-08-20T03:38:59ZengSAGE PublishingTumor Biology1423-03802017-03-013910.1177/1010428317695961Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cellsQingming Guo0Danni Zhu1Xiaocui Bu2Xiaofang Wei3Changyou Li4Daiqing Gao5Xiaoqiang Wei6Xuezhen Ma7Peng Zhao8Biotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaBiotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaThe Affiliated Cardiovascular Hospital of Qingdao University Medical College, Qingdao, ChinaBiotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaBiotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaBiotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaDepartment of Gynaecology, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaDepartment of Oncology, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaBiotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, ChinaRecurrence of breast cancer after radiotherapy may be partly explained by the presence of radioresistant cells. Thus, it would be desirable to develop an effective therapy against radioresistant cells. In this study, we demonstrated the intense antitumor activity of cytokine-induced killer cells against MCF-7 and radioresistant MCF-7 cells, as revealed by cytokine-induced killer–mediated cytotoxicity, tumor cell proliferation, and tumor invasion. Radioresistant MCF-7 cells were more susceptible to cytokine-induced killer cell killing. The stronger cytotoxicity of cytokine-induced killer cells against radioresistant MCF-7 cells was dependent on the expression of major histocompatibility complex class I polypeptide–related sequence A/B on radioresistant MCF-7 cells after exposure of cytokine-induced killer cells to sensitized targets. In addition, we demonstrated that cytokine-induced killer cell treatment sensitized breast cancer cells to chemotherapy via the downregulation of TK1, TYMS, and MDR1. These results indicate that cytokine-induced killer cell treatment in combination with radiotherapy and/or chemotherapy may induce synergistic antitumor activities and represent a novel strategy for breast cancer.https://doi.org/10.1177/1010428317695961
spellingShingle Qingming Guo
Danni Zhu
Xiaocui Bu
Xiaofang Wei
Changyou Li
Daiqing Gao
Xiaoqiang Wei
Xuezhen Ma
Peng Zhao
Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells
Tumor Biology
title Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells
title_full Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells
title_fullStr Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells
title_full_unstemmed Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells
title_short Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells
title_sort efficient killing of radioresistant breast cancer cells by cytokine induced killer cells
url https://doi.org/10.1177/1010428317695961
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