Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease
Prolactin (PRL) plays an important role in modulating the immune response. In B cells, PRL enhances antibody production, including antibodies with self-specificity. In this study, our aims were to determine the level of PRL receptor expression during bone-marrow B-cell development and to assess whe...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/287469 |
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| author | Maria Victoria Legorreta-Haquet Rocio Flores-Fernández Francisco Blanco-Favela Ezequiel M Fuentes-Pananá Luis Chávez-Sánchez Rafael Hernández-González Emiliano Tesoro-Cruz Lourdes Arriaga-Pizano Adriana Karina Chávez-Rueda |
| author_facet | Maria Victoria Legorreta-Haquet Rocio Flores-Fernández Francisco Blanco-Favela Ezequiel M Fuentes-Pananá Luis Chávez-Sánchez Rafael Hernández-González Emiliano Tesoro-Cruz Lourdes Arriaga-Pizano Adriana Karina Chávez-Rueda |
| author_sort | Maria Victoria Legorreta-Haquet |
| collection | DOAJ |
| description | Prolactin (PRL) plays an important role in modulating the immune response. In B cells, PRL enhances antibody production, including antibodies with self-specificity. In this study, our aims were to determine the level of PRL receptor expression during bone-marrow B-cell development and to assess whether the presence of high PRL serum concentrations influences absolute numbers of developing populations and disease outcome in lupus-prone murine models. We observed that the PRL-receptor is expressed in early bone-marrow B-cell; the expression in lupus-prone mice, which had the highest level of expression in pro-B cells and immature cells, differed from that in wild-type mice. These expression levels did not significantly change in response to hyperprolactinemia; however, populations of pro-B and immature cells from lupus-prone strains showed a decrease in the absolute numbers of cells with high PRL-receptor expression in response to PRL. Because immature self-reactive B cells are constantly being eliminated, we assessed the expression of survival factor BIRC5, which is more highly expressed in both pro-B and immature B-cells in response to PRL and correlates with the onset of disease. These results identify an important role of PRL in the early stages of the B-cell maturation process: PRL may promote the survival of self-reactive clones. |
| format | Article |
| id | doaj-art-daf45d823e4d4c09848296452ac596d8 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-daf45d823e4d4c09848296452ac596d82025-02-03T00:59:53ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/287469287469Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like DiseaseMaria Victoria Legorreta-Haquet0Rocio Flores-Fernández1Francisco Blanco-Favela2Ezequiel M Fuentes-Pananá3Luis Chávez-Sánchez4Rafael Hernández-González5Emiliano Tesoro-Cruz6Lourdes Arriaga-Pizano7Adriana Karina Chávez-Rueda8UIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Mexico City, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Mexico City, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Mexico City, DF, MexicoUnidad de Investigación en Virología y Cáncer Hospital Infantil de México Federico Gómez, 06720 Mexico City, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Mexico City, DF, MexicoDepartamento de Investigación Experimental y Bioterio del Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, 14000 Mexico City, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Mexico City, DF, MexicoUIM en Inmunoquímica, Hospital de Especialidades, CMN Siglo XXI, IMSS, 06720 Mexico City, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Mexico City, DF, MexicoProlactin (PRL) plays an important role in modulating the immune response. In B cells, PRL enhances antibody production, including antibodies with self-specificity. In this study, our aims were to determine the level of PRL receptor expression during bone-marrow B-cell development and to assess whether the presence of high PRL serum concentrations influences absolute numbers of developing populations and disease outcome in lupus-prone murine models. We observed that the PRL-receptor is expressed in early bone-marrow B-cell; the expression in lupus-prone mice, which had the highest level of expression in pro-B cells and immature cells, differed from that in wild-type mice. These expression levels did not significantly change in response to hyperprolactinemia; however, populations of pro-B and immature cells from lupus-prone strains showed a decrease in the absolute numbers of cells with high PRL-receptor expression in response to PRL. Because immature self-reactive B cells are constantly being eliminated, we assessed the expression of survival factor BIRC5, which is more highly expressed in both pro-B and immature B-cells in response to PRL and correlates with the onset of disease. These results identify an important role of PRL in the early stages of the B-cell maturation process: PRL may promote the survival of self-reactive clones.http://dx.doi.org/10.1155/2013/287469 |
| spellingShingle | Maria Victoria Legorreta-Haquet Rocio Flores-Fernández Francisco Blanco-Favela Ezequiel M Fuentes-Pananá Luis Chávez-Sánchez Rafael Hernández-González Emiliano Tesoro-Cruz Lourdes Arriaga-Pizano Adriana Karina Chávez-Rueda Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease Clinical and Developmental Immunology |
| title | Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease |
| title_full | Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease |
| title_fullStr | Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease |
| title_full_unstemmed | Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease |
| title_short | Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease |
| title_sort | prolactin levels correlate with abnormal b cell maturation in mrl and mrl lpr mouse models of systemic lupus erythematosus like disease |
| url | http://dx.doi.org/10.1155/2013/287469 |
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