Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus
p2 of Rice stripe virus may promote virus systemic infection by interacting with the full length of fibrillarin from Nicotiana benthamiana (NbFib2) in the nucleolus and cajal body (CB). NbFib2 contains three functional domains. We used yeast two-hybrid, colocalization, and bimolecular fluorescence c...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/8402839 |
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| _version_ | 1832559725528481792 |
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| author | Luping Zheng Jie He Zuomei Ding Chenlong Zhang Ruoxue Meng |
| author_facet | Luping Zheng Jie He Zuomei Ding Chenlong Zhang Ruoxue Meng |
| author_sort | Luping Zheng |
| collection | DOAJ |
| description | p2 of Rice stripe virus may promote virus systemic infection by interacting with the full length of fibrillarin from Nicotiana benthamiana (NbFib2) in the nucleolus and cajal body (CB). NbFib2 contains three functional domains. We used yeast two-hybrid, colocalization, and bimolecular fluorescence complementation (BiFC) assays to study the interactions between p2 and the three domains of NbFib2, namely, the N-terminal fragment containing a glycine and arginine-rich (GAR) domain, the central RNA-binding domain, and the C-terminal fragment containing an α-helical domain. The results show that the N-terminal domain is indispensable for NbFib2 to localize in the nucleolus and cajal body. p2 binds all three regions of NbFib2, and they target to the nucleus but fail to the nucleolus and cajal bodies (CBs). |
| format | Article |
| id | doaj-art-dae3b78fdcf24efb8770bc65ec98bfff |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-dae3b78fdcf24efb8770bc65ec98bfff2025-02-03T01:29:23ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932018-01-01201810.1155/2018/84028398402839Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virusLuping Zheng0Jie He1Zuomei Ding2Chenlong Zhang3Ruoxue Meng4Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Chinap2 of Rice stripe virus may promote virus systemic infection by interacting with the full length of fibrillarin from Nicotiana benthamiana (NbFib2) in the nucleolus and cajal body (CB). NbFib2 contains three functional domains. We used yeast two-hybrid, colocalization, and bimolecular fluorescence complementation (BiFC) assays to study the interactions between p2 and the three domains of NbFib2, namely, the N-terminal fragment containing a glycine and arginine-rich (GAR) domain, the central RNA-binding domain, and the C-terminal fragment containing an α-helical domain. The results show that the N-terminal domain is indispensable for NbFib2 to localize in the nucleolus and cajal body. p2 binds all three regions of NbFib2, and they target to the nucleus but fail to the nucleolus and cajal bodies (CBs).http://dx.doi.org/10.1155/2018/8402839 |
| spellingShingle | Luping Zheng Jie He Zuomei Ding Chenlong Zhang Ruoxue Meng Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus |
| title_full | Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus |
| title_fullStr | Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus |
| title_full_unstemmed | Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus |
| title_short | Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus |
| title_sort | identification of functional domain s of fibrillarin interacted with p2 of rice stripe virus |
| url | http://dx.doi.org/10.1155/2018/8402839 |
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