Stability of Human Gallbladder Bile: Effect of Freezing
In the present study, the stability of the most essential biliary parameters of human gallbladder bile at -18°C was examined over several months. In 12 patients with gallstone disease (10 female, two male; 52.1±13.3 years of age), bile was obtained through fine needle puncture of the gallbladder und...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2001-01-01
|
| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/2001/952683 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561940167131136 |
|---|---|
| author | Paul Janowitz Richard Mason Wolfgang Kratzer |
| author_facet | Paul Janowitz Richard Mason Wolfgang Kratzer |
| author_sort | Paul Janowitz |
| collection | DOAJ |
| description | In the present study, the stability of the most essential biliary parameters of human gallbladder bile at -18°C was examined over several months. In 12 patients with gallstone disease (10 female, two male; 52.1±13.3 years of age), bile was obtained through fine needle puncture of the gallbladder under local anesthetic. The concentrations of total lipids, cholesterol, phospholipids and bile acids, and the cholesterol saturation index and crystal appearance time were determined before and after freezing over a mean period of 4.38±2.9 months. Gallbladder bile obtained by fine needle puncture has proved to be of excellent quality. The total lipid concentration was unchanged before (8.30±4.16 g/dL) and after freezing (9.16±4.54 g/dL, P=0.6027). The biliary cholesterol, phospholipids and bile acid concentrations, and cholesterol saturation index showed no statistically significant differences before and after freezing. A significant difference arises in the context of subdivision of the group to the nucleation time. Before freezing, most patients had a nucleation time between five and eight days, which shortened to between one and four days after thawing (P=0.0100). The authors conclude that, with the exception of the nucleation time, human gallbladder bile can be stored at -18°C for four months with stability of major lipid components. |
| format | Article |
| id | doaj-art-da9df761b6fc434fa546abc25b4562cf |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-da9df761b6fc434fa546abc25b4562cf2025-02-03T01:23:54ZengWileyCanadian Journal of Gastroenterology0835-79002001-01-0115636336610.1155/2001/952683Stability of Human Gallbladder Bile: Effect of FreezingPaul Janowitz0Richard Mason1Wolfgang Kratzer2Department of Internal Medicine, Krankenhaus Burg, Burg, GermanyUniversity of Ulm, Department of Internal Medicine I, Ulm, GermanyUniversity of Ulm, Department of Internal Medicine I, Ulm, GermanyIn the present study, the stability of the most essential biliary parameters of human gallbladder bile at -18°C was examined over several months. In 12 patients with gallstone disease (10 female, two male; 52.1±13.3 years of age), bile was obtained through fine needle puncture of the gallbladder under local anesthetic. The concentrations of total lipids, cholesterol, phospholipids and bile acids, and the cholesterol saturation index and crystal appearance time were determined before and after freezing over a mean period of 4.38±2.9 months. Gallbladder bile obtained by fine needle puncture has proved to be of excellent quality. The total lipid concentration was unchanged before (8.30±4.16 g/dL) and after freezing (9.16±4.54 g/dL, P=0.6027). The biliary cholesterol, phospholipids and bile acid concentrations, and cholesterol saturation index showed no statistically significant differences before and after freezing. A significant difference arises in the context of subdivision of the group to the nucleation time. Before freezing, most patients had a nucleation time between five and eight days, which shortened to between one and four days after thawing (P=0.0100). The authors conclude that, with the exception of the nucleation time, human gallbladder bile can be stored at -18°C for four months with stability of major lipid components.http://dx.doi.org/10.1155/2001/952683 |
| spellingShingle | Paul Janowitz Richard Mason Wolfgang Kratzer Stability of Human Gallbladder Bile: Effect of Freezing Canadian Journal of Gastroenterology |
| title | Stability of Human Gallbladder Bile: Effect of Freezing |
| title_full | Stability of Human Gallbladder Bile: Effect of Freezing |
| title_fullStr | Stability of Human Gallbladder Bile: Effect of Freezing |
| title_full_unstemmed | Stability of Human Gallbladder Bile: Effect of Freezing |
| title_short | Stability of Human Gallbladder Bile: Effect of Freezing |
| title_sort | stability of human gallbladder bile effect of freezing |
| url | http://dx.doi.org/10.1155/2001/952683 |
| work_keys_str_mv | AT pauljanowitz stabilityofhumangallbladderbileeffectoffreezing AT richardmason stabilityofhumangallbladderbileeffectoffreezing AT wolfgangkratzer stabilityofhumangallbladderbileeffectoffreezing |