Structure-Guided design of Cas12a variants improves detection of nucleic acids
CRISPR-Cas12a holds promising potential for pathogen detection. However, its performance is not optimal when combined with isothermal amplification. Hence, we engineered a mutant of LbCas12a (K595A) with reduced cis-cleavage activity, to minimize interference with isothermal amplification. Compared...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
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| Series: | Cell Insight |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772892725000021 |
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