Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer
Water-soluble fluorescent conjugated polymer is a promising material which could be used as an optical platform in highly sensitive molecular sensors. In this paper, a simple label-free DNA sensor, which consisted of a poly(3-alkoxy-4-methylthiophene) and an aptamer, was used to detect L-argininamid...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/682134 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548495632891904 |
|---|---|
| author | Hongliang Guan Zhike He |
| author_facet | Hongliang Guan Zhike He |
| author_sort | Hongliang Guan |
| collection | DOAJ |
| description | Water-soluble fluorescent conjugated polymer is a promising material which could be used as an optical platform in highly sensitive molecular sensors. In this paper, a simple label-free DNA sensor, which consisted of a poly(3-alkoxy-4-methylthiophene) and an aptamer, was used to detect L-argininamide (L-Arm). Due to the specific binding reaction between L-Arm and its aptamer, the proposed method can easily determinate the L-Arm through the recovery of fluorescence without any modification. Other ions or similar molecules had little effect on the detection. Moreover, there was a linear relationship between fluorescence intensity and the concentration of L-Arm. The detection limit of L-Arm was as low as 4.7 nM. |
| format | Article |
| id | doaj-art-da28ff7610c9458f8ab8ec2ee85f2683 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-da28ff7610c9458f8ab8ec2ee85f26832025-02-03T06:13:57ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/682134682134Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-AptamerHongliang Guan0Zhike He1School of Environment and Civil Engineering, Wuhan Institute of Technology, Wuhan 430074, ChinaKey Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, ChinaWater-soluble fluorescent conjugated polymer is a promising material which could be used as an optical platform in highly sensitive molecular sensors. In this paper, a simple label-free DNA sensor, which consisted of a poly(3-alkoxy-4-methylthiophene) and an aptamer, was used to detect L-argininamide (L-Arm). Due to the specific binding reaction between L-Arm and its aptamer, the proposed method can easily determinate the L-Arm through the recovery of fluorescence without any modification. Other ions or similar molecules had little effect on the detection. Moreover, there was a linear relationship between fluorescence intensity and the concentration of L-Arm. The detection limit of L-Arm was as low as 4.7 nM.http://dx.doi.org/10.1155/2013/682134 |
| spellingShingle | Hongliang Guan Zhike He Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer Journal of Analytical Methods in Chemistry |
| title | Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer |
| title_full | Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer |
| title_fullStr | Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer |
| title_full_unstemmed | Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer |
| title_short | Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer |
| title_sort | determination of l argininamide based on water soluble fluorescent conjugated polymer aptamer |
| url | http://dx.doi.org/10.1155/2013/682134 |
| work_keys_str_mv | AT hongliangguan determinationoflargininamidebasedonwatersolublefluorescentconjugatedpolymeraptamer AT zhikehe determinationoflargininamidebasedonwatersolublefluorescentconjugatedpolymeraptamer |