Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin
Abstract The authors used mesoporous silica microspheres as a support for the immobilization of inulinase from Aspergillus brasiliensis MTCC 1344 by the process of cross‐linking. Under optimized operating conditions of pH 6.0, particle/enzyme ratio of 2.0:1.0 and glutaraldehyde concentration of 7 mM...
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| Format: | Article |
| Language: | English |
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Wiley
2021-04-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12031 |
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| author | Kongkona Saikia Hridya Radhakrishnan Abiram Karanam Rathankumar Siva Gokul Senthil Kumar Shravani Kalita Jenet George Sivanesan Subramanian Vaidyanathan Vinoth Kumar |
| author_facet | Kongkona Saikia Hridya Radhakrishnan Abiram Karanam Rathankumar Siva Gokul Senthil Kumar Shravani Kalita Jenet George Sivanesan Subramanian Vaidyanathan Vinoth Kumar |
| author_sort | Kongkona Saikia |
| collection | DOAJ |
| description | Abstract The authors used mesoporous silica microspheres as a support for the immobilization of inulinase from Aspergillus brasiliensis MTCC 1344 by the process of cross‐linking. Under optimized operating conditions of pH 6.0, particle/enzyme ratio of 2.0:1.0 and glutaraldehyde concentration of 7 mM, a maximum immobilization yield of 90.7% was obtained after a cross‐linking time of 12.25 h. Subsequently, the cross‐linked inulinase was utilized for the hydrolysis of 5% inulin, and a maximum fructose concentration of 31.7 g/L was achieved under the optimum conditions of pH 6.0 and temperature 60°C in 3 h. Furthermore, on performing reusability studies during inulin hydrolysis, it was observed that the immobilized inulinase could be reused up to 10 subsequent cycles of hydrolysis, thus providing a facile and commercially attractive process of high‐fructose syrup production. |
| format | Article |
| id | doaj-art-da25ae121e974628a124bf620e33ac91 |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-da25ae121e974628a124bf620e33ac912025-02-03T01:29:39ZengWileyIET Nanobiotechnology1751-87411751-875X2021-04-0115214915610.1049/nbt2.12031Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulinKongkona Saikia0Hridya Radhakrishnan1Abiram Karanam Rathankumar2Siva Gokul Senthil Kumar3Shravani Kalita4Jenet George5Sivanesan Subramanian6Vaidyanathan Vinoth Kumar7School of Bioengineering Integrated Bioprocessing Laboratory SRM Institute of Science and Technology Chennai IndiaSchool of Bioengineering Integrated Bioprocessing Laboratory SRM Institute of Science and Technology Chennai IndiaSchool of Bioengineering Integrated Bioprocessing Laboratory SRM Institute of Science and Technology Chennai IndiaSchool of Bioengineering Integrated Bioprocessing Laboratory SRM Institute of Science and Technology Chennai IndiaSchool of Bioengineering Integrated Bioprocessing Laboratory SRM Institute of Science and Technology Chennai IndiaSchool of Bioengineering Integrated Bioprocessing Laboratory SRM Institute of Science and Technology Chennai IndiaDepartment of Applied Science and Technology AC Tech Anna University Chennai IndiaSchool of Bioengineering Integrated Bioprocessing Laboratory SRM Institute of Science and Technology Chennai IndiaAbstract The authors used mesoporous silica microspheres as a support for the immobilization of inulinase from Aspergillus brasiliensis MTCC 1344 by the process of cross‐linking. Under optimized operating conditions of pH 6.0, particle/enzyme ratio of 2.0:1.0 and glutaraldehyde concentration of 7 mM, a maximum immobilization yield of 90.7% was obtained after a cross‐linking time of 12.25 h. Subsequently, the cross‐linked inulinase was utilized for the hydrolysis of 5% inulin, and a maximum fructose concentration of 31.7 g/L was achieved under the optimum conditions of pH 6.0 and temperature 60°C in 3 h. Furthermore, on performing reusability studies during inulin hydrolysis, it was observed that the immobilized inulinase could be reused up to 10 subsequent cycles of hydrolysis, thus providing a facile and commercially attractive process of high‐fructose syrup production.https://doi.org/10.1049/nbt2.12031biochemistrybiotechnologyenzymesfermentationfood processing industrymicroorganisms |
| spellingShingle | Kongkona Saikia Hridya Radhakrishnan Abiram Karanam Rathankumar Siva Gokul Senthil Kumar Shravani Kalita Jenet George Sivanesan Subramanian Vaidyanathan Vinoth Kumar Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin IET Nanobiotechnology biochemistry biotechnology enzymes fermentation food processing industry microorganisms |
| title | Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin |
| title_full | Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin |
| title_fullStr | Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin |
| title_full_unstemmed | Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin |
| title_short | Development of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin |
| title_sort | development of a sustainable route for the production of high fructose syrup from the polyfructan inulin |
| topic | biochemistry biotechnology enzymes fermentation food processing industry microorganisms |
| url | https://doi.org/10.1049/nbt2.12031 |
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