Machine learning-guided evolution of pyrrolysyl-tRNA synthetase for improved incorporation efficiency of diverse noncanonical amino acids

Machine learning-guided evolution of pyrrolysyl-tRNA synthetase for improved incorporation efficiency of diverse noncanonical amino acids

Abstract The pyrrolysyl-tRNA synthetase (PylRS) is widely used to incorporate noncanonical amino acids (ncAAs) into proteins. However, the yields of most ncAA-containing protein  remain low due to the limited activity of PylRS variants. Here, we apply machine learning to engineer the tRNA-binding do...

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Main Authors: Qunfeng Zhang, Ling Jiang, Yadan Niu, Yujie Li, Wanyi Chen, Jingxi Cheng, Haote Ding, Binbin Chen, Ke Liu, Jiawen Cao, Junli Wang, Shilin Ye, Lirong Yang, Jianping Wu, Gang Xu, Jianping Lin, Haoran Yu
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61952-2
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https://doi.org/10.1038/s41467-025-61952-2

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