Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional Methods
<i>Arthospira platensis</i> is a filamentous cyanobacterium considered an important source of phycobiliproteins (PBP), a class of water-soluble pigments with a wide range of applications. Although several extraction and purification techniques of PBP have been reported, there is still a...
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| author | Ugnė Krakauskaitė Shahana Aboobacker Vaida Kitrytė-Syrpa Michail Syrpas |
| author_facet | Ugnė Krakauskaitė Shahana Aboobacker Vaida Kitrytė-Syrpa Michail Syrpas |
| author_sort | Ugnė Krakauskaitė |
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| description | <i>Arthospira platensis</i> is a filamentous cyanobacterium considered an important source of phycobiliproteins (PBP), a class of water-soluble pigments with a wide range of applications. Although several extraction and purification techniques of PBP have been reported, there is still a need for strategies that achieve a balance between simplicity, cost-effectiveness, and scalability. To address this need, this study systematically evaluated conventional extraction methods—homogenisation, freeze–thaw cycles, and maceration. Maceration showed the best balance between yield purity and recovery among the tested techniques, and under optimal conditions (three cycles, 2 h/cycle, 0.1 M phosphate buffer), 55.9 mg/g of biomass of phycocyanin (PC) and 24.9 mg/g of biomass of allophycocyanin (APC) with notable antioxidant capacity (Folin-Ciocalteu’s and ABTS<sup>●+</sup> assays) were yielded. Among the tested purification techniques, ammonium sulphate fractionation achieved a purity of 2.3 with recoveries of 92.9% and 79.9% for PC and APC, respectively, while purification with 0.5% activated carbon resulted in an extract purity of 2.5 and recoveries of 90.9% (PC) and 87.2% (APC). The suggested procedures could be considered a fast and cost-effective way to obtain cosmetic-grade PBP, thus offering an accessible solution for industries prioritising sustainable and economically viable production pipelines. |
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| institution | Kabale University |
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| spelling | doaj-art-d9da2d8bd4e84aebb36a2955bdaf3c3a2025-01-24T13:19:43ZengMDPI AGApplied Sciences2076-34172025-01-0115253210.3390/app15020532Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional MethodsUgnė Krakauskaitė0Shahana Aboobacker1Vaida Kitrytė-Syrpa2Michail Syrpas3Department of Food Science & Technology, Faculty of Chemical Technology, Kaunas University of Technology, Radvilėnų Pl. 19, LT-50254 Kaunas, LithuaniaDepartment of Food Science & Technology, Faculty of Chemical Technology, Kaunas University of Technology, Radvilėnų Pl. 19, LT-50254 Kaunas, LithuaniaDepartment of Food Science & Technology, Faculty of Chemical Technology, Kaunas University of Technology, Radvilėnų Pl. 19, LT-50254 Kaunas, LithuaniaDepartment of Food Science & Technology, Faculty of Chemical Technology, Kaunas University of Technology, Radvilėnų Pl. 19, LT-50254 Kaunas, Lithuania<i>Arthospira platensis</i> is a filamentous cyanobacterium considered an important source of phycobiliproteins (PBP), a class of water-soluble pigments with a wide range of applications. Although several extraction and purification techniques of PBP have been reported, there is still a need for strategies that achieve a balance between simplicity, cost-effectiveness, and scalability. To address this need, this study systematically evaluated conventional extraction methods—homogenisation, freeze–thaw cycles, and maceration. Maceration showed the best balance between yield purity and recovery among the tested techniques, and under optimal conditions (three cycles, 2 h/cycle, 0.1 M phosphate buffer), 55.9 mg/g of biomass of phycocyanin (PC) and 24.9 mg/g of biomass of allophycocyanin (APC) with notable antioxidant capacity (Folin-Ciocalteu’s and ABTS<sup>●+</sup> assays) were yielded. Among the tested purification techniques, ammonium sulphate fractionation achieved a purity of 2.3 with recoveries of 92.9% and 79.9% for PC and APC, respectively, while purification with 0.5% activated carbon resulted in an extract purity of 2.5 and recoveries of 90.9% (PC) and 87.2% (APC). The suggested procedures could be considered a fast and cost-effective way to obtain cosmetic-grade PBP, thus offering an accessible solution for industries prioritising sustainable and economically viable production pipelines.https://www.mdpi.com/2076-3417/15/2/532phycocyaninextractioncyanobacteriapurification |
| spellingShingle | Ugnė Krakauskaitė Shahana Aboobacker Vaida Kitrytė-Syrpa Michail Syrpas Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional Methods Applied Sciences phycocyanin extraction cyanobacteria purification |
| title | Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional Methods |
| title_full | Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional Methods |
| title_fullStr | Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional Methods |
| title_full_unstemmed | Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional Methods |
| title_short | Optimised Extraction and Purification of Dual-Function Cosmetic-Grade Phycocyanin and Allophycocyanin from Dried <i>Arthrospira platensis</i> Biomass Using Conventional Methods |
| title_sort | optimised extraction and purification of dual function cosmetic grade phycocyanin and allophycocyanin from dried i arthrospira platensis i biomass using conventional methods |
| topic | phycocyanin extraction cyanobacteria purification |
| url | https://www.mdpi.com/2076-3417/15/2/532 |
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