STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH
Examination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in a patient's body infected with the hepati...
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| Format: | Article |
| Language: | Indonesian |
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Universitas PGRI Madiun
2020-11-01
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| Series: | Florea: Jurnal Biologi dan Pembelajarannya |
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| Online Access: | https://e-journal.unipma.ac.id/index.php/JF/article/view/7887 |
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| author | Supiana Dian Nurtjahyani Mohammad Amin Retno Handajan |
| author_facet | Supiana Dian Nurtjahyani Mohammad Amin Retno Handajan |
| author_sort | Supiana Dian Nurtjahyani |
| collection | DOAJ |
| description | Examination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in a patient's body infected with the hepatitis B virus, a fast and accurate examination is needed. PCR examination is an alternative solution to overcome this problem because the results are more accurate and valid because it can directly detect hepatitis B virus DNA as the cause of hepatitis B infection. This examination requires selecting the right primer so that accurate results are obtained. The aim of this study was to compare the use of HBS1 and HBS2 primers with P7 and P8 primers in the detection of Hepatitis B virus DNA in blood donors. The method used in this study is a laboratory experiment with Polymeration Chain Reaction (PCR) examination. The results showed that the HBS1 and HBS2 primers produced more positive HBV DNA by 76.92% compared to P7 and P8 primers, only 23.08%. Conclusion HBS1 and HBS2 primers can detect positive HBV DNA with more results. |
| format | Article |
| id | doaj-art-d8cdcc1f74d644768e072b63c5e0903a |
| institution | Kabale University |
| issn | 2355-6102 2502-0404 |
| language | Indonesian |
| publishDate | 2020-11-01 |
| publisher | Universitas PGRI Madiun |
| record_format | Article |
| series | Florea: Jurnal Biologi dan Pembelajarannya |
| spelling | doaj-art-d8cdcc1f74d644768e072b63c5e0903a2025-01-20T06:37:05ZindUniversitas PGRI MadiunFlorea: Jurnal Biologi dan Pembelajarannya2355-61022502-04042020-11-0172737610.25273/florea.v7i2.78875592STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAHSupiana Dian Nurtjahyani0Mohammad Amin1Retno Handajan2Universitas PGRI RonggolaweUniversitas AirlanggaUniversitas AirlanggaExamination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in a patient's body infected with the hepatitis B virus, a fast and accurate examination is needed. PCR examination is an alternative solution to overcome this problem because the results are more accurate and valid because it can directly detect hepatitis B virus DNA as the cause of hepatitis B infection. This examination requires selecting the right primer so that accurate results are obtained. The aim of this study was to compare the use of HBS1 and HBS2 primers with P7 and P8 primers in the detection of Hepatitis B virus DNA in blood donors. The method used in this study is a laboratory experiment with Polymeration Chain Reaction (PCR) examination. The results showed that the HBS1 and HBS2 primers produced more positive HBV DNA by 76.92% compared to P7 and P8 primers, only 23.08%. Conclusion HBS1 and HBS2 primers can detect positive HBV DNA with more results.https://e-journal.unipma.ac.id/index.php/JF/article/view/7887dna virus hepatitis bprimer hbs1 dan hbs2primer p7 dan p8 |
| spellingShingle | Supiana Dian Nurtjahyani Mohammad Amin Retno Handajan STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH Florea: Jurnal Biologi dan Pembelajarannya dna virus hepatitis b primer hbs1 dan hbs2 primer p7 dan p8 |
| title | STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH |
| title_full | STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH |
| title_fullStr | STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH |
| title_full_unstemmed | STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH |
| title_short | STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH |
| title_sort | studi perbandingan pemakaian primer hbs1 dan hbs2 dengan primer p7 dan p8 dalam deteksi dna virus hepatitis b pada pendonor darah |
| topic | dna virus hepatitis b primer hbs1 dan hbs2 primer p7 dan p8 |
| url | https://e-journal.unipma.ac.id/index.php/JF/article/view/7887 |
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