Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth
Seed coat permeability was examined using a model that tested the effects of soaking tomato (Solanum lycopersicon) seeds in combination with carbon-based nanomaterials (CBNMs) and ultrasonic irradiation (US). Penetration of seed coats to the embryo by CBNMs, as well as CBNMs effects on seed germinat...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/419215 |
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| author | Tatsiana A. Ratnikova Ramakrishna Podila Apparao M. Rao Alan G. Taylor |
| author_facet | Tatsiana A. Ratnikova Ramakrishna Podila Apparao M. Rao Alan G. Taylor |
| author_sort | Tatsiana A. Ratnikova |
| collection | DOAJ |
| description | Seed coat permeability was examined using a model that tested the effects of soaking tomato (Solanum lycopersicon) seeds in combination with carbon-based nanomaterials (CBNMs) and ultrasonic irradiation (US). Penetration of seed coats to the embryo by CBNMs, as well as CBNMs effects on seed germination and seedling growth, was examined. Two CBNMs, C60(OH)20 (fullerol) and multiwalled nanotubes (MWNTs), were applied at 50 mg/L, and treatment exposure ranged from 0 to 60 minutes. Bright field, fluorescence, and electron microscopy and micro-Raman spectroscopy provided corroborating evidence that neither CBNM was able to penetrate the seed coat. The restriction of nanomaterial (NM) uptake was attributed to the semipermeable layer located at the innermost layer of the seed coat adjacent to the endosperm. Seed treatments using US at 30 or 60 minutes in the presence of MWNTs physically disrupted the seed coat; however, the integrity of the semipermeable layer was not impaired. The germination percentage and seedling length and weight were enhanced in the presence of MWNTs but were not altered by C60(OH)20. The combined exposure of seeds to NMs and US provided insight into the nanoparticle-seed interaction and may serve as a delivery system for enhancing seed germination and early seedling growth. |
| format | Article |
| id | doaj-art-d82625531e1e4b26acba67c2d3c47c67 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d82625531e1e4b26acba67c2d3c47c672025-02-03T00:59:57ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/419215419215Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling GrowthTatsiana A. Ratnikova0Ramakrishna Podila1Apparao M. Rao2Alan G. Taylor3School of Integrative Plant Science, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456-0462, USADepartment of Physics and Astronomy, Clemson University, Clemson, SC 29634, USADepartment of Physics and Astronomy, Clemson University, Clemson, SC 29634, USASchool of Integrative Plant Science, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456-0462, USASeed coat permeability was examined using a model that tested the effects of soaking tomato (Solanum lycopersicon) seeds in combination with carbon-based nanomaterials (CBNMs) and ultrasonic irradiation (US). Penetration of seed coats to the embryo by CBNMs, as well as CBNMs effects on seed germination and seedling growth, was examined. Two CBNMs, C60(OH)20 (fullerol) and multiwalled nanotubes (MWNTs), were applied at 50 mg/L, and treatment exposure ranged from 0 to 60 minutes. Bright field, fluorescence, and electron microscopy and micro-Raman spectroscopy provided corroborating evidence that neither CBNM was able to penetrate the seed coat. The restriction of nanomaterial (NM) uptake was attributed to the semipermeable layer located at the innermost layer of the seed coat adjacent to the endosperm. Seed treatments using US at 30 or 60 minutes in the presence of MWNTs physically disrupted the seed coat; however, the integrity of the semipermeable layer was not impaired. The germination percentage and seedling length and weight were enhanced in the presence of MWNTs but were not altered by C60(OH)20. The combined exposure of seeds to NMs and US provided insight into the nanoparticle-seed interaction and may serve as a delivery system for enhancing seed germination and early seedling growth.http://dx.doi.org/10.1155/2015/419215 |
| spellingShingle | Tatsiana A. Ratnikova Ramakrishna Podila Apparao M. Rao Alan G. Taylor Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth The Scientific World Journal |
| title | Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth |
| title_full | Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth |
| title_fullStr | Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth |
| title_full_unstemmed | Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth |
| title_short | Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth |
| title_sort | tomato seed coat permeability to selected carbon nanomaterials and enhancement of germination and seedling growth |
| url | http://dx.doi.org/10.1155/2015/419215 |
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