Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation
Abstract Psoriasis is a chronic skin disorder with significant individual and societal impacts. Current therapies often lack efficacy, are costly, or cause side effects, necessitating new treatments. This study explores regenerative therapies—exosomes, mesenchymal stem cells (MSCs), epigallocatechin...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-10886-2 |
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| author | Mohamed S. Kishta Ahmed A. Abd-Rabou Garo K. Sarkissian Ahmed I. Elwakil Dana M. Elsabry Youssef M. Zagzoug Sohaila R. Hussein Ahmed N. Abdallah |
| author_facet | Mohamed S. Kishta Ahmed A. Abd-Rabou Garo K. Sarkissian Ahmed I. Elwakil Dana M. Elsabry Youssef M. Zagzoug Sohaila R. Hussein Ahmed N. Abdallah |
| author_sort | Mohamed S. Kishta |
| collection | DOAJ |
| description | Abstract Psoriasis is a chronic skin disorder with significant individual and societal impacts. Current therapies often lack efficacy, are costly, or cause side effects, necessitating new treatments. This study explores regenerative therapies—exosomes, mesenchymal stem cells (MSCs), epigallocatechin-3-gallate nanoparticles (EGN), and EGN-loaded exosomes (EGN-Exo)—in regulating psoriasis-related markers (IL-6, IL-4, Bcl-2, Bax, NF-κB, CDC25B). An imiquimod-induced psoriasis model in Wistar rats was used, with six groups: negative control, positive control, and treatments (MSCs, exosomes, EGN, EGN-Exo). After seven days, ELISA revealed EGN-Exo most effectively reduced pro-inflammatory IL-6 and pro-apoptotic Bax while increasing anti-inflammatory IL-4 and anti-apoptotic Bcl-2. EGN-Exo also significantly lowered NF-κB and CDC25B, demonstrating superior anti-inflammatory effects. Apoptosis profiling showed EGN-Exo reduced late apoptotic cells, highlighting cytoprotective abilities. EGN had a moderate effect, while MSCs and exosomes showed modest improvements. Histopathological and immunohistochemical analyses confirmed EGN-Exo’s efficacy, notably reducing TGF-β expression. These findings suggest EGN-Exo combines EGCG’s antioxidant and anti-inflammatory properties with exosomes’ targeted delivery, offering a promising advanced therapy for psoriasis. |
| format | Article |
| id | doaj-art-d806bf5129054ed08f72cf2195c9a8c0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d806bf5129054ed08f72cf2195c9a8c02025-08-20T04:03:12ZengNature PortfolioScientific Reports2045-23222025-08-0115112010.1038/s41598-025-10886-2Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulationMohamed S. Kishta0Ahmed A. Abd-Rabou1Garo K. Sarkissian2Ahmed I. Elwakil3Dana M. Elsabry4Youssef M. Zagzoug5Sohaila R. Hussein6Ahmed N. Abdallah7Hormones department, Medical Research and clinical studies institute, National Research CentreHormones department, Medical Research and clinical studies institute, National Research CentreBiotechnology Faculty, October University for Modern Sciences and Arts (MSA)Biotechnology Faculty, October University for Modern Sciences and Arts (MSA)Biotechnology Faculty, October University for Modern Sciences and Arts (MSA)Biotechnology Faculty, October University for Modern Sciences and Arts (MSA)Biotechnology Faculty, October University for Modern Sciences and Arts (MSA)Hormones department, Medical Research and clinical studies institute, National Research CentreAbstract Psoriasis is a chronic skin disorder with significant individual and societal impacts. Current therapies often lack efficacy, are costly, or cause side effects, necessitating new treatments. This study explores regenerative therapies—exosomes, mesenchymal stem cells (MSCs), epigallocatechin-3-gallate nanoparticles (EGN), and EGN-loaded exosomes (EGN-Exo)—in regulating psoriasis-related markers (IL-6, IL-4, Bcl-2, Bax, NF-κB, CDC25B). An imiquimod-induced psoriasis model in Wistar rats was used, with six groups: negative control, positive control, and treatments (MSCs, exosomes, EGN, EGN-Exo). After seven days, ELISA revealed EGN-Exo most effectively reduced pro-inflammatory IL-6 and pro-apoptotic Bax while increasing anti-inflammatory IL-4 and anti-apoptotic Bcl-2. EGN-Exo also significantly lowered NF-κB and CDC25B, demonstrating superior anti-inflammatory effects. Apoptosis profiling showed EGN-Exo reduced late apoptotic cells, highlighting cytoprotective abilities. EGN had a moderate effect, while MSCs and exosomes showed modest improvements. Histopathological and immunohistochemical analyses confirmed EGN-Exo’s efficacy, notably reducing TGF-β expression. These findings suggest EGN-Exo combines EGCG’s antioxidant and anti-inflammatory properties with exosomes’ targeted delivery, offering a promising advanced therapy for psoriasis.https://doi.org/10.1038/s41598-025-10886-2PsoriasisBone marrow mesenchymal stem cellsStem cell-derived exosomesEpigallocatechin-3-gallate nanoparticlesEpigallocatechin-3-gallate-loaded exosomes |
| spellingShingle | Mohamed S. Kishta Ahmed A. Abd-Rabou Garo K. Sarkissian Ahmed I. Elwakil Dana M. Elsabry Youssef M. Zagzoug Sohaila R. Hussein Ahmed N. Abdallah Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation Scientific Reports Psoriasis Bone marrow mesenchymal stem cells Stem cell-derived exosomes Epigallocatechin-3-gallate nanoparticles Epigallocatechin-3-gallate-loaded exosomes |
| title | Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation |
| title_full | Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation |
| title_fullStr | Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation |
| title_full_unstemmed | Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation |
| title_short | Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation |
| title_sort | exosome mediated delivery of epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation |
| topic | Psoriasis Bone marrow mesenchymal stem cells Stem cell-derived exosomes Epigallocatechin-3-gallate nanoparticles Epigallocatechin-3-gallate-loaded exosomes |
| url | https://doi.org/10.1038/s41598-025-10886-2 |
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