Efficient cytoplasmic cell quantification using a semi-automated FIJI-based tool

Abstract Quantification of subcellular structures such as nuclei and cytoplasmic proteins using staining methods based on fluorescent dyes or fluorescently tagged antibodies are widely used in scientific research. Accurate high-throughput quantitation of these assays can be time consuming and challe...

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Bibliographic Details
Main Authors: Lucas Unger, Ulrik Larsen, Shayla Sharmine, Md Kaykobad Hossain, Thomas Aga Legøy, Marc Vaudel, Luiza Ghila, Simona Chera
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-12144-x
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