Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y
Abstract: The study was carried out at Anbar Teaching Hospitals from October 2022 to September 2023 and included collect of 86 individual samples (54 males and 32 females) then, examined by using the Rose Bengal test to identify those infected with brucellosis. All individuals were divided into two...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Anbar
2024-12-01
|
| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_185840_1de1693e2981754002bbf1a75faa6715.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850194726078119936 |
|---|---|
| author | Sarah Abdullah Yassir abdulltaeef Sawsan Al-Quhli |
| author_facet | Sarah Abdullah Yassir abdulltaeef Sawsan Al-Quhli |
| author_sort | Sarah Abdullah |
| collection | DOAJ |
| description | Abstract: The study was carried out at Anbar Teaching Hospitals from October 2022 to September 2023 and included collect of 86 individual samples (54 males and 32 females) then, examined by using the Rose Bengal test to identify those infected with brucellosis. All individuals were divided into two groups, which included the control group (N= 44) who tested negative for the Rose Bengal test, and the patient's group (N= 42) tested positive for the Rose Bengal test and was suspected of having brucellosis. Brucella diagnosis by interferon-γ release assay (IGRA) was used to investigate the ability of Brucella infection to stimulate IFN-γ and compared it with the results of the Rose Bengal test. IGRA diagnosis was performed on 24 rose-Bengal test-positive blood samples isolated from the group of patients suspected of having Brucella infection and on two blood samples from the control group. Qualitative detection of Brucella-specific IgG antibodies by ELISA in the serum of all research participants. A molecular diagnosis was performed using a PCR amplification program to detect 16SrRNA genes for 28 blood samples isolated from the group of patients suspected of being infected with Brucella and for two blood samples from the control group. Finally, Analysis of data was carried out using the available statistical package of IBM SPSS-29. |
| format | Article |
| id | doaj-art-d7c8f1b5f98c4ce3acea4e8aaedf8c08 |
| institution | OA Journals |
| issn | 1991-8941 2706-6703 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj-art-d7c8f1b5f98c4ce3acea4e8aaedf8c082025-08-20T02:13:55ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032024-12-01182162210.37652/juaps.2024.146173.1181185840Extensive Diagnosis of Chronic Brucellosis Using Interferon -YSarah Abdullah0Yassir abdulltaeef1Sawsan Al-Quhli2University of Anbar, College of Medicine, Department of MicrobiologyMedical Microbiology Department, Medical College, University of Anbar, IraqMedical Microbiology Department, Medical College, University of Anbar, IraqAbstract: The study was carried out at Anbar Teaching Hospitals from October 2022 to September 2023 and included collect of 86 individual samples (54 males and 32 females) then, examined by using the Rose Bengal test to identify those infected with brucellosis. All individuals were divided into two groups, which included the control group (N= 44) who tested negative for the Rose Bengal test, and the patient's group (N= 42) tested positive for the Rose Bengal test and was suspected of having brucellosis. Brucella diagnosis by interferon-γ release assay (IGRA) was used to investigate the ability of Brucella infection to stimulate IFN-γ and compared it with the results of the Rose Bengal test. IGRA diagnosis was performed on 24 rose-Bengal test-positive blood samples isolated from the group of patients suspected of having Brucella infection and on two blood samples from the control group. Qualitative detection of Brucella-specific IgG antibodies by ELISA in the serum of all research participants. A molecular diagnosis was performed using a PCR amplification program to detect 16SrRNA genes for 28 blood samples isolated from the group of patients suspected of being infected with Brucella and for two blood samples from the control group. Finally, Analysis of data was carried out using the available statistical package of IBM SPSS-29.https://juaps.uoanbar.edu.iq/article_185840_1de1693e2981754002bbf1a75faa6715.pdfchronic brucellosisinterferon -ysensitivityspecificity |
| spellingShingle | Sarah Abdullah Yassir abdulltaeef Sawsan Al-Quhli Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y مجلة جامعة الانبار للعلوم الصرفة chronic brucellosis interferon -y sensitivity specificity |
| title | Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y |
| title_full | Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y |
| title_fullStr | Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y |
| title_full_unstemmed | Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y |
| title_short | Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y |
| title_sort | extensive diagnosis of chronic brucellosis using interferon y |
| topic | chronic brucellosis interferon -y sensitivity specificity |
| url | https://juaps.uoanbar.edu.iq/article_185840_1de1693e2981754002bbf1a75faa6715.pdf |
| work_keys_str_mv | AT sarahabdullah extensivediagnosisofchronicbrucellosisusinginterferony AT yassirabdulltaeef extensivediagnosisofchronicbrucellosisusinginterferony AT sawsanalquhli extensivediagnosisofchronicbrucellosisusinginterferony |