Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans
In this study, two low molecular mass fucoidans (LMWFs), F-1 and F-2, derived from Undaria pinnatifida fucoidan, were prepared and purified by free radical oxidation degradation and strong anion exchange chromatography. The chemical composition and structure characteristics of LMWFs were analyzed us...
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China Food Publishing Company
2025-01-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-006.pdf |
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| author | YU Fan, GUO Yingying, LI Na, DAI Xinyue, ZHU Wenjia, YAO Lin, JIANG Yanhua, WANG Lianzhu |
| author_facet | YU Fan, GUO Yingying, LI Na, DAI Xinyue, ZHU Wenjia, YAO Lin, JIANG Yanhua, WANG Lianzhu |
| author_sort | YU Fan, GUO Yingying, LI Na, DAI Xinyue, ZHU Wenjia, YAO Lin, JIANG Yanhua, WANG Lianzhu |
| collection | DOAJ |
| description | In this study, two low molecular mass fucoidans (LMWFs), F-1 and F-2, derived from Undaria pinnatifida fucoidan, were prepared and purified by free radical oxidation degradation and strong anion exchange chromatography. The chemical composition and structure characteristics of LMWFs were analyzed using molecular exclusion chromatography (MEC), high performance liquid chromatography (HPLC), infrared spectroscopy (IRS) and nuclear magnetic resonance spectroscopy (NMRS), and the structure-antioxidant activity relationship was explored. The results showed that F-1 and F-2 were primarily composed of fucose and galactose, with molecular mass of 2 790 and 23 510 Da, respectively. Their sulfate contents were (27.05 ± 0.95)% and (34.73 ± 0.08)%, and their uronic acid contents were (15.39 ± 0.33)% and (6.76 ± 0.38)%, respectively. In both F-1 and F-2, the configuration of glycosidic bonds was α-type, with a certain degree of acetylation and sulfation. Both LMWFs could scavenge 1,1-diphenyl-2-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cationic radicals and showed certain reducing power in the potassium ferricyanide reduction assay. These antioxidant properties were concentration dependent. The contents of polyuronide and galactose in the glycan chain of F-1 was relatively high, so its antioxidant activity was superior to that of F-2 under the same dosage conditions. These findings provide a theoretical basis for the application of LMWFs as excellent natural antioxidants in various fields such as health food and cosmetics. |
| format | Article |
| id | doaj-art-d7b6a13aa89a4487900d21a5d887f433 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-d7b6a13aa89a4487900d21a5d887f4332025-02-05T09:08:22ZengChina Food Publishing CompanyShipin Kexue1002-66302025-01-01462505610.7506/spkx1002-6630-20231122-173Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass FucoidansYU Fan, GUO Yingying, LI Na, DAI Xinyue, ZHU Wenjia, YAO Lin, JIANG Yanhua, WANG Lianzhu0(1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116000, China; 2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 3. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China)In this study, two low molecular mass fucoidans (LMWFs), F-1 and F-2, derived from Undaria pinnatifida fucoidan, were prepared and purified by free radical oxidation degradation and strong anion exchange chromatography. The chemical composition and structure characteristics of LMWFs were analyzed using molecular exclusion chromatography (MEC), high performance liquid chromatography (HPLC), infrared spectroscopy (IRS) and nuclear magnetic resonance spectroscopy (NMRS), and the structure-antioxidant activity relationship was explored. The results showed that F-1 and F-2 were primarily composed of fucose and galactose, with molecular mass of 2 790 and 23 510 Da, respectively. Their sulfate contents were (27.05 ± 0.95)% and (34.73 ± 0.08)%, and their uronic acid contents were (15.39 ± 0.33)% and (6.76 ± 0.38)%, respectively. In both F-1 and F-2, the configuration of glycosidic bonds was α-type, with a certain degree of acetylation and sulfation. Both LMWFs could scavenge 1,1-diphenyl-2-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cationic radicals and showed certain reducing power in the potassium ferricyanide reduction assay. These antioxidant properties were concentration dependent. The contents of polyuronide and galactose in the glycan chain of F-1 was relatively high, so its antioxidant activity was superior to that of F-2 under the same dosage conditions. These findings provide a theoretical basis for the application of LMWFs as excellent natural antioxidants in various fields such as health food and cosmetics.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-006.pdflow molecular mass fucoidans; chemical composition; structure characteristics; antioxidant activity |
| spellingShingle | YU Fan, GUO Yingying, LI Na, DAI Xinyue, ZHU Wenjia, YAO Lin, JIANG Yanhua, WANG Lianzhu Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans Shipin Kexue low molecular mass fucoidans; chemical composition; structure characteristics; antioxidant activity |
| title | Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans |
| title_full | Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans |
| title_fullStr | Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans |
| title_full_unstemmed | Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans |
| title_short | Separation, Purification, Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans |
| title_sort | separation purification structural characterization and in vitro antioxidant activity of low molecular mass fucoidans |
| topic | low molecular mass fucoidans; chemical composition; structure characteristics; antioxidant activity |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-006.pdf |
| work_keys_str_mv | AT yufanguoyingyinglinadaixinyuezhuwenjiayaolinjiangyanhuawanglianzhu separationpurificationstructuralcharacterizationandinvitroantioxidantactivityoflowmolecularmassfucoidans |