A dynamic peripheral immune landscape during human pregnancy
Extensive immune adaptations occur during pregnancy to ensure successful delivery. However, these changes can increase the risk of disease in the mother. Here, we conducted single-cell RNA sequencing on peripheral blood mononuclear cells from pregnant women at different stages of pregnancy to elucid...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2025-01-01
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| Series: | Fundamental Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667325822002813 |
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| author | Xiuxing Liu Lei Zhu Zhaohao Huang Zhaohuai Li Runping Duan He Li Lihui Xie Xiaozhen Chen Wen Ding Binyao Chen Yuehan Gao Juan Su Xianggui Wang Wenru Su |
| author_facet | Xiuxing Liu Lei Zhu Zhaohao Huang Zhaohuai Li Runping Duan He Li Lihui Xie Xiaozhen Chen Wen Ding Binyao Chen Yuehan Gao Juan Su Xianggui Wang Wenru Su |
| author_sort | Xiuxing Liu |
| collection | DOAJ |
| description | Extensive immune adaptations occur during pregnancy to ensure successful delivery. However, these changes can increase the risk of disease in the mother. Here, we conducted single-cell RNA sequencing on peripheral blood mononuclear cells from pregnant women at different stages of pregnancy to elucidate the dynamic transcriptional changes in the maternal immune system. Gradual reduced cytotoxicity phenotype in highly variable cytotoxic T and natural killer cell types were observed during pregnancy. Reduced T- and B-cell response-related MHC-II and CD40 signaling as well as enhanced protolerance inducible costimulator and activin signaling may underlie the pregnancy-related weakening of adaptive immunity. Conversely, pro-inflammatory genes and pathways were upregulated in monocytes, possibly to compensate for the reduced T-cell response. Moreover, the transition from adaptive immune reduction to activation in late pregnancy in dendritic cells and CD4+ T cells was also detected. Notably, we proposed a novel view of the pro-aging effect of pregnancy from the perspective of immunity, and this effect may be restored postpartum. This work expands our knowledge of pregnancy immunity and may provide insights into the altered disease risks during pregnancy. |
| format | Article |
| id | doaj-art-d68c051961e74b23bdac975339ecccba |
| institution | Kabale University |
| issn | 2667-3258 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Fundamental Research |
| spelling | doaj-art-d68c051961e74b23bdac975339ecccba2025-01-29T05:02:25ZengKeAi Communications Co. Ltd.Fundamental Research2667-32582025-01-0151391406A dynamic peripheral immune landscape during human pregnancyXiuxing Liu0Lei Zhu1Zhaohao Huang2Zhaohuai Li3Runping Duan4He Li5Lihui Xie6Xiaozhen Chen7Wen Ding8Binyao Chen9Yuehan Gao10Juan Su11Xianggui Wang12Wenru Su13State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaEye Center of Xiangya Hospital, Central South University, Changsha 410078, China; Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha 410008, ChinaGuangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, ChinaHunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China; Hunan Engineering Research Center of Skin Health and Disease, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China; Corresponding authors.Eye Center of Xiangya Hospital, Central South University, Changsha 410078, China; Hunan Key Laboratory of Ophthalmology, Changsha 410078, China; Corresponding authors.State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, China; Corresponding authors.Extensive immune adaptations occur during pregnancy to ensure successful delivery. However, these changes can increase the risk of disease in the mother. Here, we conducted single-cell RNA sequencing on peripheral blood mononuclear cells from pregnant women at different stages of pregnancy to elucidate the dynamic transcriptional changes in the maternal immune system. Gradual reduced cytotoxicity phenotype in highly variable cytotoxic T and natural killer cell types were observed during pregnancy. Reduced T- and B-cell response-related MHC-II and CD40 signaling as well as enhanced protolerance inducible costimulator and activin signaling may underlie the pregnancy-related weakening of adaptive immunity. Conversely, pro-inflammatory genes and pathways were upregulated in monocytes, possibly to compensate for the reduced T-cell response. Moreover, the transition from adaptive immune reduction to activation in late pregnancy in dendritic cells and CD4+ T cells was also detected. Notably, we proposed a novel view of the pro-aging effect of pregnancy from the perspective of immunity, and this effect may be restored postpartum. This work expands our knowledge of pregnancy immunity and may provide insights into the altered disease risks during pregnancy.http://www.sciencedirect.com/science/article/pii/S2667325822002813Single-cell RNA sequencingPregnancyMaternal immune systemAgingImmune toleranceDampened adaptive immunity |
| spellingShingle | Xiuxing Liu Lei Zhu Zhaohao Huang Zhaohuai Li Runping Duan He Li Lihui Xie Xiaozhen Chen Wen Ding Binyao Chen Yuehan Gao Juan Su Xianggui Wang Wenru Su A dynamic peripheral immune landscape during human pregnancy Fundamental Research Single-cell RNA sequencing Pregnancy Maternal immune system Aging Immune tolerance Dampened adaptive immunity |
| title | A dynamic peripheral immune landscape during human pregnancy |
| title_full | A dynamic peripheral immune landscape during human pregnancy |
| title_fullStr | A dynamic peripheral immune landscape during human pregnancy |
| title_full_unstemmed | A dynamic peripheral immune landscape during human pregnancy |
| title_short | A dynamic peripheral immune landscape during human pregnancy |
| title_sort | dynamic peripheral immune landscape during human pregnancy |
| topic | Single-cell RNA sequencing Pregnancy Maternal immune system Aging Immune tolerance Dampened adaptive immunity |
| url | http://www.sciencedirect.com/science/article/pii/S2667325822002813 |
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